LOCUS       sequence-98210-e         541 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of shRNA against human CENPB.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 541)
  AUTHORS   Qin H, Diaz A, Blouin L, Lebbink RJ, Patena W, Tanbun P, LeProust 
            EM, McManus MT, Song JS, Ramalho-Santos M
  TITLE     Systematic Identification of Barriers to Human iPSC Generation.
  JOURNAL   Cell. 2014 Jul 17;158(2):449-61. doi: 10.1016/j.cell.2014.05.040.
  PUBMED    25036638
REFERENCE   2  (bases 1 to 541)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2014.05"; journalName: "Cell"; date: "2014-07-17- 
            17"; volume: "158"; issue: "2"; pages: "449-61"
COMMENT     Sequence Label: pSicoR-CENPBi2
FEATURES             Location/Qualifiers
     source          1..541
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(110..143)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     147..167
                     /label=mU6-F
                     /note="Mouse U6 promoter, forward primer"
ORIGIN
        1 tttgcatata atatttccta gtaactatag aggcttaatg tgcgataaaa gacagataat
       61 ctgttctttt taatactagc tacattttac atgataggct tggatttcta taacttcgta
      121 tagcatacat tatacgaagt tataaacagc acaaaaggaa actcacccta actgtaaagt
      181 aattgtgtgt tttgagacta taaatatccc ttggagaaaa gccttgtttg tctggttcat
      241 gtgaccagga agttctagag acttcctggt cacatgaacc agactttttt ctcgagtact
      301 aggatccatt aggcggccgc gtggataacc gtattaccgc catgcattag ttattaatag
      361 taatcaatta cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt
      421 acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg
      481 acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat
      541 t
//
