LOCUS       sequence-98227-e         804 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Firefly luciferase (Promega) yeast codon-optimized yellow 
            fluorescent protein (yEVenus) fusion with PEST degron driven by RNR1
            promoter. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 804)
  AUTHORS   Mazo-Vargas A, Park H, Aydin M, Buchler NE
  TITLE     Measuring fast gene dynamics in single cells with time-lapse 
            luminescence microscopy
  JOURNAL   Mol Biol Cell. 2014 Sep 17. pii: mbc.E14-07-1187.
  PUBMED    25232010
REFERENCE   2  (bases 1 to 804)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Biol 
            Cell. 2014 Sep 17. pii: mbc.E14-07-1187."
COMMENT     Sequence Label: pNB789
FEATURES             Location/Qualifiers
     source          1..804
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     23..43
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        25..43
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 tgaccatgat tacgccaagc gcgcaattaa ccctcactaa agggaacaaa agctggagct
       61 ccaccgcggt ggcggccgtc actgtgtata gtagatctgc agctcagtca catgagacat
      121 taacgtatat aaatatataa atatatatat atccattaaa taacgaagta ggacatggtc
      181 taaaaatagt gcttctcatg tattgatttt gagtagccat aagaataatt attgatttca
      241 agatctttga tctccttaca tccaaaatta taataccact ttatggtatc acgacatcac
      301 cacaacagag aaggattgcc gcatttggat atcgtaaaca aaggcgttac catagaaatg
      361 tactgattgg cagaattact cttcaggaga atctttcata caaaggaatt ccattgggga
      421 aaatctcgtt accaagtcaa tgctgaactt tctatggcct ttgtttacta tcgttaatta
      481 ttttacgacc acttctgggt agaaatattt cgtagccctg gaacgagctt gtttacgcgt
      541 tttatcccat tatatggcac ccaaatcaaa tttaaaaaga aaaaacgcgt aaacagtgtc
      601 gggtaagttc atcctctgtt actttaattg cttctttttt tgaaattcta agtaaacgcg
      661 tcattttgat cctcaggaca cagaaatcct tgcagaatct tattgggtgt tgaatagagg
      721 acgcgtaaaa acgatatgga aatttttttc atatagtgta gaaagaatag gttggcgtag
      781 gtagtttcgt gtttgataga aacc
//
