LOCUS       sequence-98229-e        1964 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  encodes zinc finger nuclease specific to AAVS1 locus (right).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1964)
  AUTHORS   Sim X, Cardenas-Diaz FL, French DL, Gadue P
  TITLE     A Doxycycline-Inducible System for Genetic Correction of iPSC 
            Disease Models.
  JOURNAL   Methods Mol Biol. 2015 Jan 29.
  PUBMED    25630922
REFERENCE   2  (bases 1 to 1964)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods Mol
            Biol. 2015 Jan 29."
COMMENT     Sequence Label: PGK-AAVS1ZFNR
FEATURES             Location/Qualifiers
     source          1..1964
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        5..504
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(40..63)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     437..456
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     regulatory      531..540
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             543..563
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 taccgggtag gggaggcgct tttcccaagg cagtctggag catgcgcttt agcagccccg
       61 ctgggcactt ggcgctacac aagtggcctc tggcctcgca cacattccac atccaccggt
      121 aggcgccaac cggctccgtt ctttggtggc cccttcgcgc caccttctac tcctccccta
      181 gtcaggaagt tcccccccgc cccgcagctc gcgtcgtgca ggacgtgaca aatggaagta
      241 gcacgtctca ctagtctcgt gcagatggac agcaccgctg agcaatggaa gcgggtaggc
      301 ctttggggca gcggccaata gcagctttgc tccttcgctt tctgggctca gaggctggga
      361 aggggtgggt ccgggggcgg gctcaggggc gggctcaggg gcggggcggg cgcccgaagg
      421 tcctccggag gcccggcatt ctgcacgctt caaaagcgca cgtctgccgc gctgttctcc
      481 tcttcctcat ctccgggcct ttcgacctgc aagtcgaccc gcggaccggt gccaccatgg
      541 ctccaaagaa aaagaggaaa gtgggaatcc acggagtccc cgccgctatg gcagaacgac
      601 cttttcagtg cagaatttgt atgaggaact tctctcagag ctccaatctg gcaaggcaca
      661 tccgcaccca tacaggagag aaaccctttg cttgcgacat ttgtggccgc aagttcgcac
      721 gaaccgacta tctggtggat cacactaaaa tccataccgg cagtcagaag ccctttcagt
      781 gccggatttg tatgagaaac ttctcataca atacccacct gacacgacat atccggactc
      841 acaccgggga gaaacctttt gcttgcgata tttgtggacg gaagttcgca cagggctata
      901 acctggccgg gcatacaaaa atccacctga gaggctcaca gctggtgaag agcgaactgg
      961 aggagaagaa gagcgagctg aggcataaac tgaagtacgt ccctcacgag tatatcgaac
     1021 tgatcgagat tgccaggaat tccactcagg accgcattct ggaaatgaaa gtgatggagt
     1081 tctttatgaa ggtctacgga tatcgaggca aacatctggg cgggtcccgc aagcctgatg
     1141 gagctatcta caccgtgggc tctccaatcg actatggcgt gatcgtcgat acaaaggcct
     1201 acagcggagg ctataacctg cccatcggcc aggctgacga aatgcagcgg tacgtgaagg
     1261 agaaccagac tagaaataag cacattaacc caaatgaatg gtggaaagtg tatccctcta
     1321 gtgtcaccga gttcaagttt ctgttcgtgt ctgggcactt caagggaaac tacaaggcac
     1381 agctgacacg cctgaatcac aagactaact gcaatggcgc cgtgctgagt gtcgaggaac
     1441 tgctgatcgg gggagaaatg atcaaggccg gcacactgac tctggaggaa gtgcggagaa
     1501 agtttaacaa tggggagatc aatttctgag cggccgcttg cagaaattga tgatctatta
     1561 aacaataaag atgtccacta aaatggaagt ttttcctgtc atactttgtt aagaagggtg
     1621 agaacagagt acctacattt tgaatggaag gattggagct acgggggtgg gggtggggtg
     1681 ggattagata aatgcctgct ctttactgaa ggctctttac tattgcttta tgataatgtt
     1741 tcatagttgg atatcataat ttaaacaagc aaaaccaaat taagggccag ctcattcctc
     1801 ccactcatga tctatagatc tatagatctc tcgtgggatc attgtttttc tcttgattcc
     1861 cactttgtgg ttctaagtac tgtggtttcc aaatgtgtca gtttcatagc ctgaagaacg
     1921 agatcagcag cctctgttcc acatacactt cattctcagt attg
//
