LOCUS       sequence-98230-e        1964 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  encodes zinc finger nuclease specific to AAVS1 locus (left).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1964)
  AUTHORS   Sim X, Cardenas-Diaz FL, French DL, Gadue P
  TITLE     A Doxycycline-Inducible System for Genetic Correction of iPSC 
            Disease Models.
  JOURNAL   Methods Mol Biol. 2015 Jan 29.
  PUBMED    25630922
REFERENCE   2  (bases 1 to 1964)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods Mol
            Biol. 2015 Jan 29."
COMMENT     Sequence Label: PGK-AAVS1ZFNL
FEATURES             Location/Qualifiers
     source          1..1964
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        5..504
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(40..63)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     437..456
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     regulatory      531..540
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             543..563
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 taccgggtag gggaggcgct tttcccaagg cagtctggag catgcgcttt agcagccccg
       61 ctgggcactt ggcgctacac aagtggcctc tggcctcgca cacattccac atccaccggt
      121 aggcgccaac cggctccgtt ctttggtggc cccttcgcgc caccttctac tcctccccta
      181 gtcaggaagt tcccccccgc cccgcagctc gcgtcgtgca ggacgtgaca aatggaagta
      241 gcacgtctca ctagtctcgt gcagatggac agcaccgctg agcaatggaa gcgggtaggc
      301 ctttggggca gcggccaata gcagctttgc tccttcgctt tctgggctca gaggctggga
      361 aggggtgggt ccgggggcgg gctcaggggc gggctcaggg gcggggcggg cgcccgaagg
      421 tcctccggag gcccggcatt ctgcacgctt caaaagcgca cgtctgccgc gctgttctcc
      481 tcttcctcat ctccgggcct ttcgacctgc aagtcgaccc gcggaccggt gccaccatgg
      541 ctccaaagaa aaagcggaaa gtgggaatcc acggagtccc cgccgctatg gcagagagac
      601 cttttcagtg ccgaatttgt atgcggaact tctcttataa ttggcacctg cagaggcata
      661 tccgcactca caccggagag aaaccctttg catgcgacat ttgtggcaga aagttcgcca
      721 ggtccgatca tctgaccaca cacactaaaa tccataccgg ctctcagaag ccctttcagt
      781 gccgcatttg tatgcgaaac ttctctcaca attacgcccg ggactgccac atcagaacac
      841 atactgggga gaaacctttt gcttgcgata tttgtggacg aaagttcgca cagaacagta
      901 cacggatcgg ccacactaaa attcatctga gggggtctca gctggtgaag agtgaactgg
      961 aggagaagaa gagcgagctg cgccacaaac tgaagtacgt ccctcatgag tatatcgaac
     1021 tgatcgagat tgccaggaat tcaacccagg accgcattct ggaaatgaaa gtgatggagt
     1081 tctttatgaa ggtctacgga tatagaggca aacacctggg cgggtcaagg aagcctgatg
     1141 gcgctatcta cactgtgggg agcccaatcg actatggagt gattgtcgat accaaggcct
     1201 acagcggagg ctataacctg cccattggcc aggctgacga gatggaacgc tacgtggagg
     1261 aaaaccagac acgaaacaag catctgaacc caaatgaatg gtggaaggtg tatcccagct
     1321 ccgtcactga gttcaagttt ctgttcgtga gcgggcactt taaaggaaac tacaaggcac
     1381 agctgacccg gctgaatcat atcacaaact gtaatggcgc cgtgctgtcc gtcgaggaac
     1441 tgctgatcgg gggagaaatg atcaaggccg gcaccctgac actggaggaa gtgcggagaa
     1501 agtttaacaa tggggagatc aatttctgag cggccgcttg cagaaattga tgatctatta
     1561 aacaataaag atgtccacta aaatggaagt ttttcctgtc atactttgtt aagaagggtg
     1621 agaacagagt acctacattt tgaatggaag gattggagct acgggggtgg gggtggggtg
     1681 ggattagata aatgcctgct ctttactgaa ggctctttac tattgcttta tgataatgtt
     1741 tcatagttgg atatcataat ttaaacaagc aaaaccaaat taagggccag ctcattcctc
     1801 ccactcatga tctatagatc tatagatctc tcgtgggatc attgtttttc tcttgattcc
     1861 cactttgtgg ttctaagtac tgtggtttcc aaatgtgtca gtttcatagc ctgaagaacg
     1921 agatcagcag cctctgttcc acatacactt cattctcagt attg
//
