LOCUS       sequence-98231-e        9084 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Reporter construct to study unspliced RNA export. Coding sequence of
            luciferase gene was sandwiched between 5' and 3' splice site 
            sequences originating from the HIV gag/pol gene followed by CTE.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9084)
  AUTHORS   Rajanala K, Nandicoori VK
  TITLE     Localization of nucleoporin Tpr to the nuclear pore complex is 
            essential for Tpr mediated regulation of the export of unspliced 
            RNA.
  JOURNAL   PLoS One. 2012;7(1):e29921. doi: 10.1371/journal.pone.0029921. Epub 
            2012 Jan 13.
  PUBMED    22253824
REFERENCE   2  (bases 1 to 9084)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2012"; volume: "7(1):e29921. doi"; pages: " 
            10.1371/journal.pone.0029921. Epub 2012 Jan 13"
COMMENT     Sequence Label: pcDNA3-CTE-Luc
FEATURES             Location/Qualifiers
     source          1..9084
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1115..2767
                     /codon_start=1
                     /gene="luc"
                     /product="firefly luciferase"
                     /label=luciferase
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVNITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMNISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKSKL"
     primer_bind     complement(1181..1199)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     misc_feature    3071..3188
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        complement(4636..4654)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(4637..4654)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(4674..4691)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4680..4904
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4950..5378
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5037..5056)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5247..5268
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5387..5407)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5392..5722
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5573..5708
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5635..5654
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5789..6583
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5843..5862)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6453..6472
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6757..6878
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6794..6813)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6848..6867
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6927..6943)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6927..6943)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6940..6962)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6951..6967
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6975..6981,6982..6999,7000..7005))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7020..7041
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7158..7175)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7329..7917)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7409..7428)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8088..8879,8880..8948))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8711..8730
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8949..9053)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttggtacc
      901 gagctcggat ccactagtaa cggccgccag tgtgctggaa ttctgcagat atccatcaca
      961 ctggcggccg cccccttcac cgctagcgag gagctctctc gacgcaggac tcggcttgct
     1021 gaagcgcgca cggcaagagg cgaggggcgg cgactggtga gtacgccaaa aattttgact
     1081 agcggaggct agaaggagag agatgggtaa gcttatggaa gacgccaaaa acataaagaa
     1141 aggcccggcg ccattctatc ctctggagga tggaaccgct ggagagcaac tgcataaggc
     1201 tatgaagaga tacgccctgg ttcctggaac aattgctttt acagatgcac atatcgaggt
     1261 gaacatcacg tacgcggaat acttcgaaat gtccgttcgg ttggcagaag ctatgaaacg
     1321 atatgggctg aatacaaatc acagaatcgt cgtatgcagt gaaaactctc ttcaattctt
     1381 tatgccggtg ttgggcgcgt tatttatcgg agttgcagtt gcgcccgcga acgacattta
     1441 taatgaacgt gaattgctca acagtatgaa catttcgcag cctaccgtag tgtttgtttc
     1501 caaaaagggg ttgcaaaaaa ttttgaacgt gcaaaaaaaa ttaccaataa tccagaaaat
     1561 tattatcatg gattctaaaa cggattacca gggatttcag tcgatgtaca cgttcgtcac
     1621 atctcatcta cctcccggtt ttaatgaata cgattttgta ccagagtcct ttgatcgtga
     1681 caaaacaatt gcactgataa tgaattcctc tggatctact gggttaccta agggtgtggc
     1741 ccttccgcat agaactgcct gcgtcagatt ctcgcatgcc agagatccta tttttggcaa
     1801 tcaaatcatt ccggatactg cgattttaag tgttgttcca ttccatcacg gttttggaat
     1861 gtttactaca ctcggatatt tgatatgtgg atttcgagtc gtcttaatgt atagatttga
     1921 agaagagctg tttttacgat cccttcagga ttacaaaatt caaagtgcgt tgctagtacc
     1981 aaccctattt tcattcttcg ccaaaagcac tctgattgac aaatacgatt tatctaattt
     2041 acacgaaatt gcttctgggg gcgcacctct ttcgaaagaa gtcggggaag cggttgcaaa
     2101 acgcttccat cttccaggga tacgacaagg atatgggctc actgagacta catcagctat
     2161 tctgattaca cccgaggggg atgataaacc gggcgcggtc ggtaaagttg ttccattttt
     2221 tgaagcgaag gttgtggatc tggataccgg gaaaacgctg ggcgttaatc agagaggcga
     2281 attatgtgtc agaggaccta tgattatgtc cggttatgta aacaatccgg aagcgaccaa
     2341 cgccttgatt gacaaggatg gatggctaca ttctggagac atagcttact gggacgaaga
     2401 cgaacacttc ttcatagttg accgcttgaa gtctttaatt aaatacaaag gatatcaggt
     2461 ggcccccgct gaattggaat cgatattgtt acaacacccc aacatcttcg acgcgggcgt
     2521 ggcaggtctt cccgacgatg acgccggtga acttcccgcc gccgttgttg ttttggagca
     2581 cggaaagacg atgacggaaa aagagatcgt ggattacgtc gccagtcaag taacaaccgc
     2641 gaaaaagttg cgcggaggag ttgtgtttgt ggacgaagta ccgaaaggtc ttaccggaaa
     2701 actcgacgca aggaaaatca gagagatcct cataaaggcc aagaagggcg gaaagtccaa
     2761 attgtaaggt accagcagaa gttattccag cagaaacagg gcaggaaaca gcatattttc
     2821 ttttaaaatt agcaggaaga tggccagtaa aaacaataca tacagacaat ggcagcaatt
     2881 tcaccagtgc tacggttaag gccgcctgtt ggtgggcggg aatcaagcag gaatttggaa
     2941 ttccctacaa tccccaaagt caaggagtag tagaatctat gaataaagaa ttaaagaaaa
     3001 ttataggaca ggtaagagat caggctgaac atcttaagac agcagtacaa atggcagtat
     3061 tcatccacaa ttttaaaaga aaagggggga ttggggggta cagtgcaggg gaaagaatag
     3121 tagacataat agcaacagac atacaaacta aagaattaca aaaacaaatt acaaaaattc
     3181 aaaattttcg ggtttattac agggacagca gaaatccact ttggaaagga ccagcaaagc
     3241 tcctctggaa aggtgaaggg gcagtagtaa tacaagataa tagtgacata aaagtagtgc
     3301 caagaagaaa agcaaagatc attagggatt atggaaaaca gatggcaggt gatgattgtg
     3361 tggcaagtag acaggatgag gattagaaca tggaaaagtt tagtaaaaca ccatatgtat
     3421 gtttcaggga aagctagggg atggttttat agacatcact atgaaagccc tcatccaaga
     3481 ataagttcag aagtacacat cccactaggg gatgctagat tggtaataac aacatattgg
     3541 ggtctgcata caggagaaag agactggcat ttgggtcagg gagtctccat agaatggagg
     3601 aaaaagagat atagcacaca agtagaccct gaactagcag accaactaat tcatctgtat
     3661 tactttgact gtttttcaga ctctgctata agaaaggcct tattaggaca catagttagc
     3721 cctaggtgtg aatatcaagc aggacataac aaggtaggat ctctacaata cttggcacta
     3781 gcagcattaa taacaccaaa aaagataaag ccacctttgc ctagtgttac gaaactgaca
     3841 gaggatagat ggaacaagcc ccagaagacc aagggccaca gagggagcca cacaatgaat
     3901 ggacactaga gcttttagag gagcttaaga atgaagctgt tagacatttt cctaggattt
     3961 ggctccatgg cttagggcaa catatctatg aaacttatgg ggatacttgg gcaggagtgg
     4021 aagccataat aagaattctg caacaactgc tgtttatcca ttttcagaat tgggtgtcga
     4081 gatatcacct cccctgtgag ctagactgga cagccaatga cgggtaagag agtgacattt
     4141 ctcactaacc taagacagga gggccgtcaa agctactgcc taatccaatg acgggtaata
     4201 gtgacaagaa atgtatcact ccaacctaag acaggcgcag cctccgaggg atgtgtcttt
     4261 tgttttttat aattaaaaag ggtgacatgt ccggagccgt gctgcccgga tgatgtcttg
     4321 gcctctgttt gctctagctc catgttatga atttaagatg gcgtatttcc tggttcttct
     4381 ccgtcttact ttcccgccgg cgcgaatgtt tcccgctctt gggcttacgt ggctttcctt
     4441 gctctgctac tgagcatgcg cccagtatct ttcccctccc acttgctgcc tgtgtatata
     4501 aggcaacaca ttgccaccat taaatgagac ttgatcagaa cactgtcttg tctccatttc
     4561 ttgtgtctct tgttcccttc aattcccact ccctcctcca ggttcctact gttgatcccg
     4621 tctagagggc cctattctat agtgtcacct aaatgctaga gctcgctgat cagcctcgac
     4681 tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct
     4741 ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct
     4801 gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg
     4861 ggaagacaat agcaggcatg ctggggatgc ggtgggctct atggcttctg aggcggaaag
     4921 aaccagctgg ggctctaggg ggtatcccca cgcgccctgt agcggcgcat taagcgcggc
     4981 gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc
     5041 tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa
     5101 tcggggcatc cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact
     5161 tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt
     5221 gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa
     5281 ccctatctcg gtctattctt ttgatttata agggattttg gggatttcgg cctattggtt
     5341 aaaaaatgag ctgatttaac aaaaatttaa cgcgaattaa ttctgtggaa tgtgtgtcag
     5401 ttagggtgtg gaaagtcccc aggctcccca ggcaggcaga agtatgcaaa gcatgcatct
     5461 caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
     5521 aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc
     5581 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta
     5641 tgcagaggcc gaggccgcct ctgcctctga gctattccag aagtagtgag gaggcttttt
     5701 tggaggccta ggcttttgca aaaagctccc gggagcttgt atatccattt tcggatctga
     5761 tcaagagaca ggatgaggat cgtttcgcat gattgaacaa gatggattgc acgcaggttc
     5821 tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga caatcggctg
     5881 ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac
     5941 cgacctgtcc ggtgccctga atgaactgca ggacgaggca gcgcggctat cgtggctggc
     6001 cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg gaagggactg
     6061 gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg ctcctgccga
     6121 gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc cggctacctg
     6181 cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga tggaagccgg
     6241 tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt
     6301 cgccaggctc aaggcgcgca tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc
     6361 ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg actgtggccg
     6421 gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata ttgctgaaga
     6481 gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg ctcccgattc
     6541 gcagcgcatc gccttctatc gccttcttga cgagttcttc tgagcgggac tctggggttc
     6601 gaaatgaccg accaagcgac gcccaacctg ccatcacgag atttcgattc caccgccgcc
     6661 ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat gatcctccag
     6721 cgcggggatc tcatgctgga gttcttcgcc caccccaact tgtttattgc agcttataat
     6781 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat
     6841 tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctgtat accgtcgacc
     6901 tctagctaga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg
     6961 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa
     7021 tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac
     7081 ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
     7141 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
     7201 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
     7261 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
     7321 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     7381 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     7441 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     7501 tcgggaagcg tggcgctttc tcaatgctca cgctgtaggt atctcagttc ggtgtaggtc
     7561 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     7621 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     7681 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     7741 tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag
     7801 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     7861 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     7921 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     7981 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
     8041 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
     8101 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
     8161 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
     8221 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
     8281 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
     8341 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
     8401 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
     8461 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
     8521 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
     8581 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
     8641 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
     8701 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
     8761 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
     8821 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
     8881 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
     8941 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
     9001 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
     9061 ccccgaaaag tgccacctga cgtc
//
