LOCUS       sequence-98232-e       12501 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses Flag-Tagged nucleoporin Tpr in Mammalian Cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12501)
  AUTHORS   Vomastek T, Iwanicki MP, Burack WR, Tiwari D, Kumar D, Parsons JT, 
            Weber MJ, Nandicoori VK
  TITLE     Extracellular signal-regulated kinase 2 (ERK2) phosphorylation sites
            and docking domain on the nuclear pore complex protein Tpr 
            cooperatively regulate ERK2-Tpr interaction.
  JOURNAL   Mol Cell Biol. 2008 Nov;28(22):6954-66. doi: 10.1128/MCB.00925-08. 
            Epub 2008 Sep 15.
  PUBMED    18794356
REFERENCE   2  (bases 1 to 12501)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1128/MCB.00925-08"; journalName: "Mol Cell Biol"; date: 
            "2008-11"; volume: "28"; issue: "22"; pages: "6954-66"
COMMENT     Sequence Label: pCDNA3-Flag-Tpr
FEATURES             Location/Qualifiers
     source          1..12501
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      945..954
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             954..977
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     promoter        complement(8053..8071)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(8054..8071)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(8091..8108)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8097..8321
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      8367..8795
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8454..8473)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8664..8685
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8804..8824)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8809..9139
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8990..9125
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     9052..9071
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             9206..10000
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(9260..9279)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     9870..9889
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    10174..10295
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10211..10230)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10265..10284
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(10344..10360)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10344..10360)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10357..10379)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10368..10384
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10392..10398,10399..10416,10417..10422))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10437..10458
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10575..10592)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10746..11334)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10826..10845)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11505..12296,12297..12365))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12128..12147
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12366..12470)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttggtacc
      901 gagctcggat ccactagtaa cggccgccag tgtgctggaa ttctgccacc atggactaca
      961 aggacgacga tgacaagcca gcggccgcta tggcggcggt gttgcagcaa gtcctggagc
     1021 gcacggagct gaacaagctg cccaagtctg tccagaacaa acttgaaaag ttccttgctg
     1081 atcagcaatc cgagatcgat ggcctgaagg ggcggcatga gaaatttaag gtggagagcg
     1141 aacaacagta ttttgaaata gaaaagaggt tgtcccacag tcaggagaga cttgtgaatg
     1201 aaacccgaga gtgtcaaagc ttgcggcttg agctagagaa actcaacaat caactgaagg
     1261 cactaactga gaaaaacaaa gaacttgaaa ttgctcagga tcgcaatatt gccattcaga
     1321 gccaatttac aagaacaaag gaagaattag aagctgagaa aagagactta attagaacca
     1381 atgagagact atctcaagaa cttgaatact taacagagga tgttaaacgt ctgaatgaaa
     1441 aacttaaaga aagcaataca acaaagggtg aacttcagtt aaaattggat gaacttcaag
     1501 cttctgatgt ttctgttaag tatcgagaaa aacgcttgga gcaagaaaag gaattgctac
     1561 atagtcagaa tacatggctg aatacagagt tgaaaaccaa aactgatgaa cttctggctc
     1621 ttggaagaga aaaagggaat gagattctag agcttaaatg taatcttgaa aataaaaaag
     1681 aagaggtttc tagactggaa gaacaaatga atggcttaaa aacatcaaat gaacatcttc
     1741 aaaagcatgt ggaggatctg ttgaccaaat taaaagaggc caaggaacaa caggccagta
     1801 tggaagagaa attccacaat gaattaaatg cccacataaa actttctaat ttgtacaaga
     1861 gtgccgctga tgactcagaa gcaaagagca atgaactaac ccgggcagta gaggaactac
     1921 acaaactttt gaaagaagct ggtgaagcca acaaagcaat acaagatcat cttctagagg
     1981 tggagcaatc caaagatcaa atggaaaaag aaatgcttga gaaaataggg agattggaga
     2041 aggaattaga gaatgcaaat gaccttcttt ctgccacaaa acgtaaagga gccatattgt
     2101 ctgaagaaga gcttgccgcc atgtctccta ctgcagcagc tgtagctaag atagtgaaac
     2161 ctgggatgaa actaactgag ctctataatg cttatgtgga aactcaggat cagttgcttt
     2221 tggagaaact agagaacaaa agaattaata agtacctaga tgaaatagtg aaagaagtgg
     2281 aagccaaagc accaattttg aaacgccagc gtgaggaata tgaacgtgca cagaaagctg
     2341 tagcaagttt atctgttaag cttgaacaag ctatgaagga gattcagcga ttgcaggagg
     2401 acactgataa agccaacaag caatcatctg tacttgagag agataatcga agaatggaaa
     2461 tacaagtaaa agatctttca caacagatta gagtgctttt gatggaactt gaagaagcaa
     2521 ggggtaacca cgtaattcgt gatgaggaag taagctctgc tgatataagt agttcatctg
     2581 aggtaatatc acagcatcta gtatcttaca gaaatattga agagcttcaa caacaaaatc
     2641 aacgtctctt agtggccctt agagagcttg gggaaaccag agaaagagaa gaacaagaaa
     2701 caacttcatc caaaatcact gagcttcagc tcaaacttga gagtgccctt actgaactag
     2761 aacaactccg caaatcacga cagcatcaaa tgcagcttgt tgattccata gttcgtcagc
     2821 gtgatatgta ccgtatttta ttgtcacaaa caacaggagt tgccattcca ttacatgctt
     2881 caagcttaga tgatgtttct cttgcatcaa ctccaaaacg tccaagtaca tcacagactg
     2941 tttccactcc tgctccagta cctgttattg aatcaacaga ggctatagag gctaaggctg
     3001 cccttaaaca gttgcaggaa atttttgaga actacaaaaa agaaaaagca gaaaatgaaa
     3061 aaatacaaaa tgagcagctt gagaaacttc aagaacaagt tacagatttg cgatcacaaa
     3121 ataccaaaat ttctacccag ctagattttg cttctaaacg ttatgaaatg ctgcaagata
     3181 atgttgaagg atatcgtcga gaaataacat cacttcatga gagaaatcag aaactcactg
     3241 ccacaactca aaagcaagaa cagattatca atacgatgac tcaagatttg agaggagcaa
     3301 atgagaagct agctgtcgca gaagtaagag cagaaaattt gaagaaggaa aaggaaatgc
     3361 ttaaattgtc tgaagttcgt ctttctcagc aaagagagtc tttgttagct gaacaaaggg
     3421 ggcaaaactt actgctaact aatctgcaaa caattcaggg aatactggag cgatctgaaa
     3481 cagaaaccaa acaaaggctt agtagccaga tagaaaaact ggaacatgag atctctcatc
     3541 taaagaagaa gttggaaaat gaggtggaac aaaggcatac acttactaga aatctagatg
     3601 ttcaactttt agatacaaag agacaactgg atacagagac aaatcttcat cttaacacaa
     3661 aagaactatt aaaaaatgct caaaaagaaa ttgccacatt gaaacagcac ctcagtaata
     3721 tggaagtcca agttgcttct cagtcttcac agagaactgg taaaggtcag cctagcaaca
     3781 aagaagatgt ggatgatctt gtgagtcagc taagacagac agaagagcag gtgaatgact
     3841 taaaggagag actcaaaaca agtacgagca atgtggaaca atatcaagca atggttacta
     3901 gtttagaaga atccctgaac aaggaaaaac aggtgacaga agaagtgcgt aagaatattg
     3961 aagttcgttt aaaagagtca gctgaatttc agacacagtt ggaaaagaag ttgatggaag
     4021 tagagaagga aaaacaagaa cttcaggatg ataaaagaag agccatagag agcatggaac
     4081 aacagttatc tgaattgaag aaaacacttt ctagtgttca gaatgaagta caagaagctc
     4141 ttcagagagc aagcacagct ttaagtaatg agcagcaagc cagacgtgac tgtcaggaac
     4201 aagctaaaat agctgtggaa gctcagaata agtatgagag agaattgatg ctgcatgctg
     4261 ctgatgttga agctctacaa gctgcgaagg agcaggtttc aaaaatggca tcagtccgtc
     4321 agcatttgga agaaacaaca cagaaagcag aatcacagtt gttggagtgt aaagcatctt
     4381 gggaggaaag agagagaatg ttaaaggatg aagtttccaa atgtgtatgt cgctgtgaag
     4441 atctggagaa acaaaacaga ttacttcatg atcagatcga aaaattaagt gacaaggtcg
     4501 ttgcctctgt gaaggaaggt gtacaaggtc cactgaatgt atctctcagt gaagaaggaa
     4561 aatctcaaga acaaattttg gaaattctca gatttatacg acgagaaaaa gaaattgctg
     4621 aaactaggtt tgaggtggct caggttgaga gtctgcgtta tcgacaaagg gttgaacttt
     4681 tagaaagaga gctgcaggaa ctcgaagata gtctaaatgc tgaaagggag aaagtccagg
     4741 taactgcaaa aacaatggct cagcatgaag aactgatgaa gaaaactgaa acaatgaatg
     4801 tagttatgga gaccaataaa atgctaagag aagagaagga gagactagaa caggatctac
     4861 agcaaatgca agcaaaggtg aggaaactgg agttagatat tttaccctta caagaagcaa
     4921 atgctgagct gagtgagaaa agcggtatgt tgcaggcaga gaagaagctc ttagaagagg
     4981 atgtcaaacg ttggaaagca cgtaaccagc atctagtaag tcaacagaaa gatccagata
     5041 cagaagaata tcggaagctc ctttctgaaa aggaagttca tactaagcgt attcaacaat
     5101 tgacagaaga aattggtaga cttaaagctg aaattgcaag atcaaatgca tctttgacta
     5161 acaaccagaa cttaattcag agtctgaagg aagatctaaa taaagtaaga actgaaaagg
     5221 aaaccatcca gaaggactta gatgccaaaa taattgatat ccaagaaaaa gtcaaaacta
     5281 ttactcaagt taagaaaatt ggacgtaggt acaagactca atatgaagaa cttaaagcac
     5341 aacaggataa ggttatggag acatcggctc agtcctctgg agaccatcag gagcagcatg
     5401 tttcagtcca ggaaatgcag gaactcaaag aaacgctcaa ccaagctgaa acaaaatcaa
     5461 aatcacttga aagtcaagta gagaatctgc agaagacatt atctgaaaaa gagacagaag
     5521 caagaaatct ccaggaacag actgtgcaac ttcagtctga actttcacga cttcgtcagg
     5581 atcttcaaga tagaaccaca caggaggagc agctccgaca acagataact gaaaaggaag
     5641 aaaaaaccag aaaggctatt gtagcagcaa agtcaaaaat tgcacactta gctggtgtaa
     5701 aagatcagct aactaaagaa aatgaggagc ttaaacaaag gaatggagcc ttagatcagc
     5761 agaaagatga attggatgtt cgcattactg cgctaaagtc ccaatatgaa ggtcgaatta
     5821 gtcgcttgga aagagaactc agggagcatc aagagagaca ccttgagcag agagatgagc
     5881 ctcaagaacc ttctaataag gtccctgaac agcagagaca gatcacattg aaaacaactc
     5941 cagcttctgg tgaaagagga attgccagca catcagaccc accaacagcc aatatcaagc
     6001 caactcctgt tgtgtctact ccaagtaaag tgacagctgc agctatggct ggaaataagt
     6061 caacacccag ggctagtatc cgcccaatgg ttacacctgc aactgttaca aatcccacta
     6121 ctaccccaac agctacagtg atgcccacta cacaagtgga atcacaggaa gctatgcagt
     6181 cagaagggcc tgtggaacat gttccagttt ttggaagcac aagtggatcc gttcgttcta
     6241 ctagtcctaa tgtccagcct tctatctctc aacctatttt aactgttcag caacaaacac
     6301 aggctacagc ttttgtgcaa cccactcaac agagtcatcc tcagattgag cctgccaatc
     6361 aagagttatc ttcaaacata gtagaggttg ttcagagttc accagttgag cggccttcta
     6421 cttccacagc agtatttggc acagtttcgg ctacccccag ttcttctttg ccaaagcgta
     6481 cacgtgaaga ggaagaggat agcaccatag aagcatcaga ccaagtctct gatgatacag
     6541 tggaaatgcc tcttccaaag aagttgaaaa gtgtcacacc tgtaggaact gaggaagaag
     6601 ttatggcaga agaaagtact gatggagagg tagagactca ggtatacaac caggattctc
     6661 aagattccat tggagaagga gttacccagg gagattatac acctatggaa gacagtgaag
     6721 aaacctctca gtctctacaa atagatcttg ggccacttca atcagatcag cagacgacaa
     6781 cttcatccca ggatggtcaa ggcaaaggag atgatgtcat tgtaattgac agtgatgatg
     6841 aagaagagga tgaggaagat gatgatgatg atgaagatga cacagggatg ggagatgagg
     6901 gtgaagatag taatgaagga actggtagtg ccgatggcaa tgatggttat gaagctgatg
     6961 atgctgaggg tggtgatggg actgatccag gtacagaaac agaagaaagt atgggtggag
     7021 gtgaaggtaa tcacagagct gctgattctc aaaacagtgg tgaaggaaat acaggtgctg
     7081 cagaatcttc tttttctcag gaggtttcta gagaacaaca gccatcatca gcatctgaaa
     7141 gacaggcccc tcgagcacct cagtcaccga gacgcccacc acatccactt cccccaagac
     7201 tgaccattca tgccccacct caggagttgg gaccaccagt tcagagaatt cagatgaccc
     7261 gaaggcagtc tgtaggacgt ggccttcagt tgactccagg aataggtggc atgcaacagc
     7321 atttttttga tgatgaagac agaacagttc caagtactcc aactcttgtg gtgccacatc
     7381 gtactgatgg atttgctgaa gcaattcatt cgccgcaggt tgctggtgtc cctagattcc
     7441 ggtttgggcc acctgaagat atgccacaaa caagttctag tcactctgat cttggccagc
     7501 ttgcttctca aggaggttta ggaatgtatg aaacacccct gttcctagct catgaagaag
     7561 agtcaggtgg ccgaagtgtt cccactactc cactacaagt agcagcccca gtgactgtat
     7621 ttactgagag caccacctct gatgcttcgg aacatgcctc tcaatctgtt ccaatggtga
     7681 ctacatccac tggcacttta tctacaacaa atgaaacagc aacaggtgat gatggagatg
     7741 aagtatttgt ggaggcagaa tctgaaggta ttagttcaga agcaggccta gaaattgata
     7801 gccagcagga agaagagccg gttcaagcat ctgatgagtc agatctcccc tccaccagcc
     7861 aggatcctcc ttctagctca tctgtagata ctagtagtag tcaaccaaag cctttcagac
     7921 gagtaagact tcagacaaca ttgagacaag gtgtccgtgg tcgtcagttt aacagacaga
     7981 gaggtgtgag ccatgcaatg ggagggagag gaggaataaa cagaggaaat attaattaag
     8041 ggccctattc tatctatagt gtcacctaaa tgctagagct cgctgatcag cctcgactgt
     8101 gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga
     8161 aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag
     8221 taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga
     8281 agacaatagc aggcatgctg gggatgcggt gggctctatg gcttctgagg cggaaagaac
     8341 cagctggggc tctagggggt atccccacgc gccctgtagc ggcgcattaa gcgcggcggg
     8401 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt
     8461 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg
     8521 gggcatccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga
     8581 ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac
     8641 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc
     8701 tatctcggtc tattcttttg atttataagg gattttgggg atttcggcct attggttaaa
     8761 aaatgagctg atttaacaaa aatttaacgc gaattaattc tgtggaatgt gtgtcagtta
     8821 gggtgtggaa agtccccagg ctccccaggc aggcagaagt atgcaaagca tgcatctcaa
     8881 ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
     8941 catgcatctc aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct
     9001 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc
     9061 agaggccgag gccgcctctg cctctgagct attccagaag tagtgaggag gcttttttgg
     9121 aggcctaggc ttttgcaaaa agctcccggg agcttgtata tccattttcg gatctgatca
     9181 agagacagga tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg caggttctcc
     9241 ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa tcggctgctc
     9301 tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg tcaagaccga
     9361 cctgtccggt gccctgaatg aactgcagga cgaggcagcg cggctatcgt ggctggccac
     9421 gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa gggactggct
     9481 gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc ctgccgagaa
     9541 agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg ctacctgccc
     9601 attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg aagccggtct
     9661 tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg aactgttcgc
     9721 caggctcaag gcgcgcatgc ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg
     9781 cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact gtggccggct
     9841 gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg ctgaagagct
     9901 tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc ccgattcgca
     9961 gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga gcgggactct ggggttcgaa
    10021 atgaccgacc aagcgacgcc caacctgcca tcacgagatt tcgattccac cgccgccttc
    10081 tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc
    10141 ggggatctca tgctggagtt cttcgcccac cccaacttgt ttattgcagc ttataatggt
    10201 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
    10261 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctgtatacc gtcgacctct
    10321 agctagagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc
    10381 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga
    10441 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg
    10501 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg
    10561 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
    10621 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
    10681 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
    10741 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
    10801 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
    10861 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
    10921 ggaagcgtgg cgctttctca atgctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
    10981 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
    11041 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
    11101 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
    11161 tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca
    11221 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
    11281 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
    11341 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
    11401 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
    11461 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
    11521 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
    11581 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
    11641 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
    11701 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
    11761 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
    11821 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
    11881 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
    11941 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
    12001 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
    12061 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
    12121 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
    12181 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
    12241 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
    12301 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
    12361 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
    12421 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
    12481 cgaaaagtgc cacctgacgt c
//
