LOCUS       sequence-98250-e        1026 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Green beetle luciferase with nuclear localization signal (NLS) and 
            ClpXP degron tag (ssrA).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1026)
  AUTHORS   Mazo-Vargas A, Park H, Aydin M, Buchler NE
  TITLE     Measuring fast gene dynamics in single cells with time-lapse 
            luminescence microscopy
  JOURNAL   Mol Biol Cell. 2014 Sep 17. pii: mbc.E14-07-1187.
  PUBMED    25232010
REFERENCE   2  (bases 1 to 1026)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Biol 
            Cell. 2014 Sep 17. pii: mbc.E14-07-1187."
COMMENT     Sequence Label: pNB774
FEATURES             Location/Qualifiers
     source          1..1026
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             54..74
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 cgggctaatt atggggtgtc gcccttcgct gaatttaatt aaccatggct ccaccaaaaa
       61 aaaaaagaaa agttgccatg ggtgggggca gcatgatgaa gagagaaaaa aatgttgttt
      121 acggccccga acccaaacac cctttgggaa actttactgc tggagaaatg ctctacaatg
      181 cccttcataa gcattcccac ataccgcaag caatattaga tgtgatgggt aatgaatcgc
      241 tgtcatatca agaatttttc gacactactg tcaagctagg acaaagtctt caaaattgtg
      301 gatacaagat gaatgatgta gtgtcgatct gtgctgagaa caataaaaga tttttcatcc
      361 ccattatttc agcttggtat attggtatgg ttgtagcacc tgttaatgaa gactacatcc
      421 cagatgaact ttgtaaagtc acgggtatat caaaaccaat actggtcttc actacaagaa
      481 aaatcttacc taaggtatta gaggtaaagg acagaactaa ttacataaag agaattataa
      541 tactagattc tgaagaaaat ctgcttggtt gcgaaagtct tcataatttt atgtcccgtt
      601 attcggataa taatcttcag acctttaagc ctctacatta cgatcctgtt gatcaagtgg
      661 cagctatttt atgttcgtca ggcacaactg gattacccaa aggtgttatg caaacgcata
      721 gaaacatttg tgttcgactt acacatgctt cggatcccag agttggaaca caacttattc
      781 ctggagtatc agttttggcg tatctgcctt tcttccatgc ttttgggttt tctataaact
      841 taggatactt tatggtgggc cttcgtgttg ttatgctaag acgatttaat caagaagtat
      901 ttttaaaagc cattcaagat tatgaagttc gaagtgtaat caacgttcca tcaacaatac
      961 tgttcttgtc gaaaagtcct ttagttgaca aatacgattt atcgactttg gcggaattgt
     1021 gttgcg
//
