LOCUS       sequence-98252-e         725 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 725)
  AUTHORS   Hampel S, Chung P, McKellar CE, Hall D, Looger LL, Simpson JH
  TITLE     Drosophila Brainbow: a recombinase-based fluorescence labeling 
            technique to subdivide neural expression patterns.
  JOURNAL   Nat Methods. 2011 Mar;8(3):253-9. doi: 10.1038/nmeth.1566. Epub 2011
            Feb 6.
  PUBMED    21297621
REFERENCE   2  (bases 1 to 725)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.1566"; journalName: "Nat Methods"; date: "2011-03"; 
            volume: "8"; issue: "3"; pages: "253-9"
COMMENT     Sequence Label: dBrainbow
FEATURES             Location/Qualifiers
     source          1..725
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             3..35
                     /codon_start=1
                     /product="HSV (herpes simplex virus) epitope tag"
                     /label=HSV tag
                     /translation="QPELAPEDPED"
     protein_bind    55..88
                     /label=lox5171
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTGTAC) (Shaw et al., 2021). lox5171 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
ORIGIN
        1 aacagcccga gctggccccc gaggatcccg aggattgacg taagctagct cgagataact
       61 tcgtataatg tgtactatac gaagttatca aaatggtgag caagggcgag gagctcacaa
      121 tgggcgtaat caagcccgac atgaagatca agctgaagat ggagggcaac gtgaatggcc
      181 acgccttcgt gatcgagggc gagggcgagg gcaagcccta cgacggcacc aacaccatca
      241 acctggaggt gaaggaggga gcccccctgc ccttctccta cgacattctg accaccgcgt
      301 tcgcctacgg caacagggcc ttcaccaagt accccgacga catccccaac tacttcaagc
      361 agtccttccc cgagggctac tcttgggagc gcaccatgac cttcgaggac aagggcatcg
      421 tgaaggtgaa gtccgacatc tccatggagg aggactcctt catctacgag atacacctca
      481 agggcgagaa cttccccccc aacggccccg tgatgcagaa gaagaccacc ggctgggacg
      541 cctccaccga gaggatgtac gtgcgcgacg gcgtgctgaa gggcgacgtc aagcacaagc
      601 tgctgctgga gggcggcggc caccaccgcg ttgacttcaa gaccatctac agggccaaga
      661 aggcggtgaa gctgcccgac tatcactttg tggaccaccg catcgagatc ctgaaccacg
      721 acaag
//
