LOCUS       sequence-98253-e         717 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 717)
  AUTHORS   Hampel S, Chung P, McKellar CE, Hall D, Looger LL, Simpson JH
  TITLE     Drosophila Brainbow: a recombinase-based fluorescence labeling 
            technique to subdivide neural expression patterns.
  JOURNAL   Nat Methods. 2011 Mar;8(3):253-9. doi: 10.1038/nmeth.1566. Epub 2011
            Feb 6.
  PUBMED    21297621
REFERENCE   2  (bases 1 to 717)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.1566"; journalName: "Nat Methods"; date: "2011-03"; 
            volume: "8"; issue: "3"; pages: "253-9"
COMMENT     Sequence Label: dBrainbow
FEATURES             Location/Qualifiers
     source          1..717
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(17..50)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
ORIGIN
        1 ctcattttgc ggccgcataa cttcgtatag gatactttat acgaagttat cgtcttgcag
       61 ttcatcctcg catggttttg cctaattgtt tcagatttat ggggcttaca tacatacata
      121 attactttat acaacgaaaa ttactaaata gaatcgttcc tgtaagcagg actgacacat
      181 ttgcttgcgt tgattgctat tatatcgacc taccatagtc acataaatat gtacatatat
      241 aaatatgtat gtatgtacaa tcgcatgctt gacggcagtt aagaaatgaa gtcaccattt
      301 cggtgagctg gcaacgatta tgctgtggcc ccgaaaggaa tgactcatcg tcccagagaa
      361 tcgataccaa cggtggaaag ccaagtgcat gccacactgc gtgcacagcc tgctgctaca
      421 cttgccacaa acagcaggcg gaaaagcgca gcagcggccc gaatcgggga aaatagaaag
      481 cgctaattat agacgaagtg aacggcagct ctgctttccc gcagtcgaaa ctccgtgcaa
      541 tgaccccgtt ctttcaccac gaaatgaatg atcgctggga gaaagaaaag gcaaagaaaa
      601 acatagtggg gttttcttat ttctgacaac actgaatctg gccgatttta ttcttgtgta
      661 cacaacttat cgccgagtta acttggcttt tctgctatac gtgtctttgt ggataaa
//
