LOCUS       sequence-98254-e         896 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Non-integrating (episomal) expression vector with Gateway cassette.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 896)
  AUTHORS   Okita K, Yamakawa T, Matsumura Y, Sato Y, Amano N, Watanabe A, 
            Goshima N, Yamanaka S
  TITLE     An Efficient Non-viral Method to Generate Integration-Free Human iPS
            Cells from Cord Blood and Peripheral Blood Cells.
  JOURNAL   Stem Cells. 2012 Nov 29. doi: 10.1002/stem.1293.
  PUBMED    23193063
REFERENCE   2  (bases 1 to 896)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Stem Cells.
            2012 Nov 29. doi: 10.1002/stem.1293."
COMMENT     Sequence Label: pCXLE-gw
FEATURES             Location/Qualifiers
     source          1..896
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     49..68
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     protein_bind    100..224
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(249..254,255..272,273..279)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     primer_bind     complement(399..418)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 cctctgctaa ccatgttcat gccttcttct ttttcctaca gctcctgggc aacgtgctgg
       61 ttattgtgct gtctcatcat tttggcaaag aattatcaaa caagtttgta caaaaaagct
      121 gaacgagaaa cgtaaaatga tataaatatc aatatattaa attagatttt gcataaaaaa
      181 cagactacat aatactgtaa aacacaacat atccagtcac tatggcggcc gcattaggca
      241 ccccaggctt tacactttat gcttccggct cgtataatgt gtggattttg agttaggatc
      301 cgtcgagatt ttcaggagct aaggaagcta aaatggagaa aaaaatcact ggatatacca
      361 ccgttgatat atcccaatgg catcgtaaag aacattttga ggcatttcag tcagttgctc
      421 aatgtaccta taaccagacc gttcagctgg atattacggc ctttttaaag accgtaaaga
      481 aaaataagca caagttttat ccggccttta ttcacattct tgcccgcctg atgaatgctc
      541 atccggaatt ccgtatggca atgaaagacg gtgagctggt gatatgggat agtgttcacc
      601 cttgttacac cgttttccat gagcaaactg aaacgttttc atcgctctgg agtgaatacc
      661 acgacgattt ccggcagttt ctacacatat attcgcaaga tgtggcgtgt tacggtgaaa
      721 acctggccta tttccctaaa gggtttattg agaatatgtt tttcgtctca gccaatccct
      781 gggtgagttt caccagtttt gatttaaacg tggccaatat ggacaacttc ttcgcccccg
      841 ttttcaccat gggcaaatat tatacgcaag gcgacaaggt gctgatgccg ctggcg
//
