LOCUS       sequence-98257-e         795 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Contains a mutant version of the WT pSynSRE-T-Luc reporter plasmid. 
            Four point mutations in the promoter SRE elements abolish 
            SREBP-induced luciferase expression as described by Smith et al., 
            1988.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 795)
  AUTHORS   Smith JR, Osborne TF, Brown MS, Goldstein JL, Gil G
  TITLE     Multiple sterol regulatory elements in promoter for hamster 
            3-hydroxy-3-methylglutaryl-coenzyme A synthase.
  JOURNAL   J Biol Chem. 1988 Dec 5;263(34):18480-7.
  PUBMED    2903862
REFERENCE   2  (bases 1 to 795)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "1988-12-5"; volume: "263(34)"; pages: "18480-7"
COMMENT     Sequence Label: pSynSRE-Mut-T-Luc
FEATURES             Location/Qualifiers
     source          1..795
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    299..420
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(310..329)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     364..383
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(747..768)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 ggcgccgggc ctttctttat gtttttggcg tcttccattt taccaacagt accggaatgc
       61 caagcttggg ggcaaagaaa ggagcaatcc acggagcaca gtgcactgtg cctcccgccc
      121 tcagcagctt tatagttgac cttgtcgagc tagcgacaaa gtcggaggac gggggaagtg
      181 gggtgagacg gtgacggagg ggggcccgag aacatctcgg ccaatggggg gagggtgaga
      241 gacggcgaga gccagagctc ggtacctccc gggttatgtt agctcagtta cagtaccata
      301 agatacattg atgagtttgg acaaaccaca actagaatgc agtgaaaaaa atgctttatt
      361 tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt
      421 aacaacaaca attgcattca ttttatgttt caggttcagg gggaggtgtg ggaggttttt
      481 taaagcaagt aaaacctcca cgtaccttaa tattacttac ttatcatggt agcttgggct
      541 ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg
      601 gcgaatggca aattgtaagc gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa
      661 tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat cccttataaa tcaaaagaat
      721 agaccgagat agggttgagt gttgttccag tttggaacaa gagtccacta ttaaagaacg
      781 tggactccaa cgtca
//
