LOCUS       sequence-98270-e         949 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Cre recombinase and Renilla luciferase from the EFS 
            promoter and one U6-driven sgRNA targeting LacZ. AAV backbone..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 949)
  AUTHORS   Platt RJ, Chen S, Zhou Y, Yim MJ, Swiech L, Kempton HR, Dahlman JE, 
            Parnas O, Eisenhaure TM, Jovanovic M, Graham DB, Jhunjhunwala S, 
            Heidenreich M, Xavier RJ, Langer R, Anderson DG, Hacohen N, Regev A,
            Feng G, Sharp PA, Zhang F
  TITLE     CRISPR-Cas9 Knockin Mice for Genome Editing and Cancer Modeling.
  JOURNAL   Cell. 2014 Sep 24. pii: S0092-8674(14)01163-5. doi: 
            10.1016/j.cell.2014.09.014.
  PUBMED    25263330
REFERENCE   2  (bases 1 to 949)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2014 
            Sep 24. pii: S0092-8674(14)01163-5. doi: 
            10.1016/j.cell.2014.09.014."
COMMENT     Sequence Label: 
            AAV:ITR-U6-sgRNA(LacZ)-pEFS-Rluc-2A-Cre-WPRE-hGHpA-ITR
FEATURES             Location/Qualifiers
     source          1..949
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     128..147
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        227..302
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        363..574
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
ORIGIN
        1 caaggctgtt agagagataa ttggaattaa tttgactgta aacacaaaga tattagtaca
       61 aaatacgtga cgtagaaagt aataatttct tgggtagttt gcagttttaa aattatgttt
      121 taaaatggac tatcatatgc ttaccgtaac ttgaaagtat ttcgatttct tggctttata
      181 tatcttgtgg aaaggacgaa acaccgtgcg aatacgccca cgcgatgttt tagagctaga
      241 aatagcaagt taaaataagg ctagtccgtt atcaacttga aaaagtggca ccgagtcggt
      301 gctttttttc tagaggtacc aggtcttgaa aggagtggga attggctccg gtgcccgtca
      361 gtgggcagag cgcacatcgc ccacagtccc cgagaagttg gggggagggg tcggcaattg
      421 aaccggtgcc tagagaaggt ggcgcggggt aaactgggaa agtgatgtcg tgtactggct
      481 ccgccttttt cccgagggtg ggggagaacc gtatataagt gcagtagtcg ccgtgaacgt
      541 tctttttcgc aacgggtttg ccgccagaac acaggcgtac ggccaccatg acttcgaaag
      601 tttatgatcc agaacaaagg aaacggatga taactggtcc gcagtggtgg gccagatgta
      661 aacaaatgaa tgttcttgat tcatttatta attattatga ttcagaaaaa catgcagaaa
      721 atgctgttat ttttttacat ggtaacgcgg cctcttctta tttatggcga catgttgtgc
      781 cacatattga gccagtagcg cggtgtatta taccagacct tattggtatg ggcaaatcag
      841 gcaaatctgg taatggttct tataggttac ttgatcatta caaatatctt actgcatggt
      901 ttgaacttct taatttacca aagaagatca tttttgtcgg ccatgattg
//
