LOCUS       sequence-98273-e         715 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Cre recombinase and Renilla luciferase from the EFS 
            promoter and one U6-driven sgRNA targeting LacZ. AAV backbone..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 715)
  AUTHORS   Platt RJ, Chen S, Zhou Y, Yim MJ, Swiech L, Kempton HR, Dahlman JE, 
            Parnas O, Eisenhaure TM, Jovanovic M, Graham DB, Jhunjhunwala S, 
            Heidenreich M, Xavier RJ, Langer R, Anderson DG, Hacohen N, Regev A,
            Feng G, Sharp PA, Zhang F
  TITLE     CRISPR-Cas9 Knockin Mice for Genome Editing and Cancer Modeling.
  JOURNAL   Cell. 2014 Sep 24. pii: S0092-8674(14)01163-5. doi: 
            10.1016/j.cell.2014.09.014.
  PUBMED    25263330
REFERENCE   2  (bases 1 to 715)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2014 
            Sep 24. pii: S0092-8674(14)01163-5. doi: 
            10.1016/j.cell.2014.09.014."
COMMENT     Sequence Label: 
            AAV:ITR-U6-sgRNA(LacZ)-pEFS-Rluc-2A-Cre-WPRE-hGHpA-ITR
FEATURES             Location/Qualifiers
     source          1..715
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(54..265)
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     misc_RNA        complement(326..401)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        complement(423..671)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     complement(481..500)
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(651..671)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
ORIGIN
        1 tccgtttcct ttgttctgga tcataaactt tcgaagtcat ggtggccgta cgcctgtgtt
       61 ctggcggcaa acccgttgcg aaaaagaacg ttcacggcga ctactgcact tatatacggt
      121 tctcccccac cctcgggaaa aaggcggagc cagtacacga catcactttc ccagtttacc
      181 ccgcgccacc ttctctaggc accggttcaa ttgccgaccc ctccccccaa cttctcgggg
      241 actgtgggcg atgtgcgctc tgcccactga cgggcaccgg agccaattcc cactcctttc
      301 aagacctggt acctctagaa aaaaagcacc gactcggtgc cactttttca agttgataac
      361 ggactagcct tattttaact tgctatttct agctctaaaa catcgcgtgg gcgtattcgc
      421 acggtgtttc gtcctttcca caagatatat aaagccaaga aatcgaaata ctttcaagtt
      481 acggtaagca tatgatagtc cattttaaaa cataatttta aaactgcaaa ctacccaaga
      541 aattattact ttctacgtca cgtattttgt actaatatct ttgtgtttac agtcaaatta
      601 attccaatta tctctctaac agccttgtat cgtatatgca aatatgaagg aatcatggga
      661 aataggccct cacgcgtgcg gccgcaggaa cccctagtga tggagttggc cactc
//
