LOCUS       sequence-98284-e         937 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Cre recombinase and KASH-tagged EGFP from the hSyn 
            promoter and one U6-driven sgRNA. AAV backbone with SapI spacer for 
            sgRNA cloning..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 937)
  AUTHORS   Platt RJ, Chen S, Zhou Y, Yim MJ, Swiech L, Kempton HR, Dahlman JE, 
            Parnas O, Eisenhaure TM, Jovanovic M, Graham DB, Jhunjhunwala S, 
            Heidenreich M, Xavier RJ, Langer R, Anderson DG, Hacohen N, Regev A,
            Feng G, Sharp PA, Zhang F
  TITLE     CRISPR-Cas9 Knockin Mice for Genome Editing and Cancer Modeling.
  JOURNAL   Cell. 2014 Sep 24. pii: S0092-8674(14)01163-5. doi: 
            10.1016/j.cell.2014.09.014.
  PUBMED    25263330
REFERENCE   2  (bases 1 to 937)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2014 
            Sep 24. pii: S0092-8674(14)01163-5. doi: 
            10.1016/j.cell.2014.09.014."
COMMENT     Sequence Label: 
            AAV:ITR-U6-sgRNA(NeuN)-hSyn-Cre-2A-EGFP-KASH-WPRE-shortPA-ITR
FEATURES             Location/Qualifiers
     source          1..937
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             46..66
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 tcgtgtcgtg cctgagagcg cagtcgagaa ggtaccgcca ccatgcccaa gaagaagagg
       61 aaggtgtcca atctcctgac tgttcaccag aacctccctg cgctgccagt agatgccact
      121 agcgatgagg tcaggaaaaa tctcatggat atgtttaggg atagacaggc gttttctgaa
      181 cacacctgga aaatgctgct tagcgtgtgc cgatcctggg cagcctggtg taagctgaac
      241 aatcgcaaat ggttccccgc cgagccggag gacgtgcgcg attacctgct gtatctccag
      301 gcaagagggc tggctgtcaa gactatccag cagcacttgg gccaactgaa tatgctgcat
      361 cgacgcagcg ggctcccccg gcctagcgat tcaaacgcag tctcccttgt tatgaggaga
      421 attagaaagg aaaacgtaga tgcgggtgag agggctaagc aggctctcgc ttttgagcgg
      481 actgatttcg accaggtcag atccctgatg gagaacagcg atcggtgcca ggacatcagg
      541 aacctcgcat ttctgggaat tgcatataac acacttctgc gcatagctga gatcgcccgg
      601 atcagagtga aagacatcag tcgaacggac ggcggccgga tgcttattca tattggacgc
      661 acaaagacat tggtcagcac cgctggcgtt gaaaaggcct tgtccctggg cgtaacgaag
      721 ctggtggaaa gatggatctc agtgtccggc gtggctgacg accctaataa ttacttgttc
      781 tgtcgagtga gaaaaaacgg agtcgccgcg ccctctgcca ccagccaatt gagtacacgg
      841 gcccttgaag ggatctttga ggcaacccac cgactcatat acggagccaa ggatgacagt
      901 ggccagaggt atctcgcctg gtcaggtcat tctgcta
//
