LOCUS       sequence-98293-e        9957 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  A minimal K560 kinesin rigor motor tagged with the SunTag for single
            molecule imaging.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9957)
  AUTHORS   Tanenbaum ME, Gilbert LA, Qi LS, Weissman JS, Vale RD
  TITLE     A Protein-Tagging System for Signal Amplification in Gene Expression
            and Fluorescence Imaging.
  JOURNAL   Cell. 2014 Oct 8. pii: S0092-8674(14)01227-6. doi: 
            10.1016/j.cell.2014.09.039.
  PUBMED    25307933
REFERENCE   2  (bases 1 to 9957)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2014 
            Oct 8. pii: S0092-8674(14)01227-6. doi: 10.1016/j.cell.2014.09.039."
COMMENT     Sequence Label: pcDNA4TO-K560-E236A-24xGCN4_v1-IRES-Puro
FEATURES             Location/Qualifiers
     source          1..9957
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     720..740
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     promoter        725..769
                     /label=CMVd3 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields strongly reduced expression 
                     relative to the full-length promoter"
     protein_bind    771..789
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    792..810
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     820..844
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      953..962
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2720..2785
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             2801..2866
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             2882..2947
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             2963..3028
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             3044..3109
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             3125..3190
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             3206..3271
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             3287..3352
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             3368..3433
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             3449..3514
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             3530..3595
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             3611..3676
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     primer_bind     3692..3714
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
     primer_bind     3715..3737
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             3740..3805
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             3821..3886
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             3902..3967
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             3983..4048
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             4064..4129
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             4145..4210
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             4226..4291
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             4307..4372
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             4388..4453
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             4469..4534
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             4550..4615
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             4631..4696
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     misc_feature    4767..5319
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4911..4928)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5138..5157
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             5331..5930
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5331..5350)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5827..5847
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     complement(5968..5985)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5974..6198
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6244..6672
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6331..6350)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6541..6562
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6681..6701)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6686..7015
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6866..7001
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6928..6947
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        7063..7110
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7129..7503
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    7633..7754
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7670..7689)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7724..7743
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7803..7819)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7803..7819)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7816..7838)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7827..7843
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7851..7857,7858..7875,7876..7881))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7896..7917
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8034..8051)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8205..8790)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8285..8304)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8961..9752,9753..9821))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9584..9603
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9822..9926)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcag ctcagctttc tagaccgcgc
      721 gcaaatgggc ggtaggcgtg tacggtggga ggtctatata agcagagctc tccctatcag
      781 tgatagagat ctccctatca gtgatagaga tcgtcgacga gctcgtttag tgaaccgtca
      841 gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagacacc gggaccgatc
      901 cagcctccgg actctagcgt ttaaacttaa gcttggtacc gagctcggat ccgccaccat
      961 ggcggacctg gccgagtgca acatcaaagt gatgtgtcgc ttcagacctc tcaacgagtc
     1021 tgaagtgaac cgcggcgaca agtacatcgc caagtttcag ggagaagaca cggtcgtgat
     1081 cgcgtccaag ccttatgcat ttgatcgggt gttccagtca agcacatctc aagagcaagt
     1141 gtataatgac tgtgcaaaga agattgttaa agatgtactt gaaggatata atggaacaat
     1201 atttgcatac ggccaaacat cctctgggaa gacacacaca atggagggta aacttcatga
     1261 tccagaaggc atgggaatta ttccaagaat agtgcaagat atttttaatt atatttactc
     1321 gatggatgaa aatttggaat ttcatattaa ggtttcatat tttgaaatat atttggataa
     1381 gataagggac ctgttagatg tttcaaagac caacctttca gttcatgaag acaaaaaccg
     1441 agttccctat gtaaaggggt gcacagagcg ttttgtatgt agtccagatg aagttatgga
     1501 taccatagat gaaggaaaat ccaacagaca tgtagcagtt acaaatatga atgaacatag
     1561 ctctaggagt cacagtatat ttcttattaa tgtcaaacaa gagaacacac aaacggaaca
     1621 aaagctgagt ggaaaacttt atctggttga tttagctggt agtgcgaagg ttagtaaaac
     1681 tggagctgaa ggtgctgtgc tggatgaagc taaaaacatc aacaagtcac tttctgctct
     1741 tggaaatgtt atttctgctt tggctgaggg tagtacttat gttccatatc gagatagtaa
     1801 aatgacaaga atccttcaag attcattagg tggcaactgt agaaccacta ttgtaatttg
     1861 ctgctctcca tcatcataca atgagtctga aacaaaatct acactcttat ttggccaaag
     1921 agccaaaaca attaagaaca cagtttgtgt caatgtggag ttaactgcag aacagtggaa
     1981 aaagaagtat gaaaaagaaa aagaaaaaaa taagatcctg cggaacacta ttcagtggct
     2041 tgaaaatgag ctcaacagat ggcgtaatgg ggagacggtg cctattgatg aacagtttga
     2101 caaagagaaa gccaacttgg aagctttcac agtggataaa gatattactc ttaccaatga
     2161 taaaccagca accgcaattg gagttatagg aaattttact gatgctgaaa gaagaaagtg
     2221 tgaagaagaa attgctaaat tatacaaaca gcttgatgac aaggatgaag aaattaacca
     2281 gcaaagtcaa ctggtagaga agctgaagac gcaaatgttg gatcaggagg agcttttggc
     2341 atctaccaga agggatcaag acaatatgca agctgagctg aatcgccttc aagcagaaaa
     2401 tgatgcctct aaagaagaag tgaaagaagt tttacaggcc ctagaagaac ttgctgtcaa
     2461 ttatgatcag aagtctcagg aagttgaaga caaaactaag gaatatgaat tgcttagtga
     2521 tgaattgaat caggaatcgg caactttagc gagtatagat gctgagcttc agaaacttaa
     2581 ggaaatgacc aaccaccaga aaaaacgagc agctgagatg atggcatctt tactaaaagg
     2641 taccgcggcc gcagatcgat cgatcacgcg tgccaccatg actagttttg tacaaagcgg
     2701 tccgggaggt tctggaggat tgttgcccaa aaactaccac ttggagaacg aggtcgcaag
     2761 gttgaaaaaa ttggtcggtg agcgtggcgg ctcaggcggc cttcttccaa aaaattatca
     2821 tcttgaaaat gaagttgcta gacttaaaaa acttgttgga gaaagaggcg ggagcggcgg
     2881 cctgctgccc aagaactacc acctggagaa cgaggtcgcc cgcctgaaga agctggtcgg
     2941 cgagcgcggt ggttctggag gtttgctccc aaagaactac cacttggaaa acgaagtcgc
     3001 aagattgaag aagttggtcg gtgaaagagg aggaagcgga ggattattac ctaaaaatta
     3061 tcatttggaa aatgaagttg ctagattgaa gaagttggtt ggagagagag gggggtctgg
     3121 aggactacta ccgaaaaact accacctaga gaacgaggtg gcccgactaa aaaagctagt
     3181 gggcgagcgc ggcggcagtg gcggcctcct ccccaagaat taccacctcg aaaatgaagt
     3241 agcaaggctc aagaagctcg taggagaaag aggtggttcc gggggactcc ttccaaaaaa
     3301 ctatcatctt gaaaacgaag ttgcacgtct taaaaaactt gttggcgaac gtggcggctc
     3361 tggcgggctg ctgcccaaga attaccacct ggaaaatgaa gtggcccgcc tgaagaagct
     3421 ggtgggcgaa cgcggaggca gtggaggcct actaccgaag aactaccacc tagagaacga
     3481 ggtggcacgc ctaaagaagc tagtgggcga gcgcggcggc tcgggtggtt tattacctaa
     3541 gaattatcat ttagaaaatg aagtagctcg attaaagaag ttagtaggtg aacgtggtgg
     3601 gtctggtggt cttcttccga agaactacca ccttgagaac gaggtagctc gtcttaagaa
     3661 acttgtaggt gagcgtggtg gaagcggtgg tgggctggca agccacgttt ggtgccggga
     3721 gctgcatgtg tcagaggaac ttttgccaaa gaattatcat cttgagaacg aagtggctcg
     3781 tcttaagaaa ctcgtaggag aaagaggtgg cagtggaggt ctgcttccca agaattacca
     3841 cctggaaaat gaggtagcta gactgaaaaa gctcgtaggc gagcgcgggg gaagtggggg
     3901 attgctgcca aaaaattatc atttggagaa cgaagtagca cgactaaaga aattagttgg
     3961 ggagaggggg ggatcgggtg gcttactccc gaaaaattat catctcgaaa acgaagtggc
     4021 tcggctaaaa aagttagttg gagagcgtgg cggttctgga ggcctattac caaaaaacta
     4081 ccacctcgaa aatgaggtgg cacgcttaaa aaagctggtc ggagaacggg gaggcagtgg
     4141 tggcctacta cccaagaatt atcatcttga gaacgaggta gcgcgtttga agaagctcgt
     4201 cggtgaaaga ggtggctcag gagggctgct cccaaagaac tatcatcttg aaaatgaggt
     4261 cgctcgatta aaaaagttag tgggcgagag gggaggcagt ggtgggctac ttcctaagaa
     4321 ttaccacctc gaaaacgaag tagctcgatt aaagaaattg gtcggtgaga gaggtgggtc
     4381 gggtggctta ctgccgaaaa attatcatct ggaaaatgag gtagcgagac taaaaaagct
     4441 cgtaggtgag agagggggtt ctggcggctt gctaccaaaa aattatcatc ttgagaacga
     4501 agttgctagg ctcaaaaagc tggttggaga gcgcggcggc tcaggcggtc tgttacccaa
     4561 aaactaccac ttggaaaatg aggttgccag attgaagaaa ctggtcggcg agcgcggtgg
     4621 aagtggagga ctacttccta aaaattatca tttggagaac gaagtcgcta gactgaaaaa
     4681 gttggttggg gaacgaggag ggtctggcgg caagctagtc gaattcgaca cgtaggctag
     4741 cttagtaggc ggccgccccc cccctaacgt tactggccga agccgcttgg aataaggccg
     4801 gtgtgcgttt gtctatatgt tattttccac catattgccg tcttttggca atgtgagggc
     4861 ccggaaacct ggccctgtct tcttgacgag cattcctagg ggtctttccc ctctcgccaa
     4921 aggaatgcaa ggtctgttga atgtcgtgaa ggaagcagtt cctctggaag cttcttgaag
     4981 acaaacaacg tctgtagcga ccctttgcag gcagcggaac cccccacctg gcgacaggtg
     5041 cctctgcggc caaaagccac gtgtataaga tacacctgca aaggcggcac aaccccagtg
     5101 ccacgttgtg agttggatag ttgtggaaag agtcaaatgg ctctcctcaa gcgtattcaa
     5161 caaggggctg aaggatgccc agaaggtacc ccattgtatg ggatctgatc tggggcctcg
     5221 gtgcacatgc tttacatgtg tttagtcgag gttaaaaaaa cgtctaggcc ccccgaacca
     5281 cggggacgtg gttttccttt gaaaaacacg atgataatat ggccacaacc atgaccgagt
     5341 acaagcccac ggtgcgcctc gccacccgcg acgacgtccc ccgggccgta cgcaccctcg
     5401 ccgccgcgtt cgccgactac cccgccacgc gccacaccgt cgacccggac cgccacatcg
     5461 agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg
     5521 tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac cacgccggag agcgtcgaag
     5581 cgggggcggt gttcgccgag atcggcccgc gcatggccga gttgagcggt tcccggctgg
     5641 ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt
     5701 tcctggccac cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc agcgccgtcg
     5761 tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg gagacctccg
     5821 cgccccgcaa cctccccttc tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg
     5881 tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc cggtgcctga ctcgagtcta
     5941 gagggcccgt ttaaacccgc tgatcagcct cgactgtgcc ttctagttgc cagccatctg
     6001 ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt
     6061 cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg
     6121 gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg
     6181 atgcggtggg ctctatggct tctgaggcgg aaagaaccag ctggggctct agggggtatc
     6241 cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
     6301 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
     6361 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
     6421 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg
     6481 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
     6541 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt
     6601 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
     6661 ttaacgcgaa ttaattctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc
     6721 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa
     6781 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
     6841 catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc
     6901 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctctgcctc
     6961 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct
     7021 cccgggagct tgtatatcca ttttcggatc tgatcagcac gtgttgacaa ttaatcatcg
     7081 gcatagtata tcggcatagt ataatacgac aaggtgagga actaaaccat ggccaagttg
     7141 accagtgccg ttccggtgct caccgcgcgc gacgtcgccg gagcggtcga gttctggacc
     7201 gaccggctcg ggttctcccg ggacttcgtg gaggacgact tcgccggtgt ggtccgggac
     7261 gacgtgaccc tgttcatcag cgcggtccag gaccaggtgg tgccggacaa caccctggcc
     7321 tgggtgtggg tgcgcggcct ggacgagctg tacgccgagt ggtcggaggt cgtgtccacg
     7381 aacttccggg acgcctccgg gccggccatg accgagatcg gcgagcagcc gtgggggcgg
     7441 gagttcgccc tgcgcgaccc ggccggcaac tgcgtgcact tcgtggccga ggagcaggac
     7501 tgacacgtgc tacgagattt cgattccacc gccgccttct atgaaaggtt gggcttcgga
     7561 atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat gctggagttc
     7621 ttcgcccacc ccaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc
     7681 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc
     7741 atcaatgtat cttatcatgt ctgtataccg tcgacctcta gctagagctt ggcgtaatca
     7801 tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga
     7861 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt
     7921 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga
     7981 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc
     8041 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
     8101 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
     8161 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     8221 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     8281 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     8341 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
     8401 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     8461 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     8521 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     8581 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     8641 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     8701 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtttttttgt ttgcaagcag
     8761 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     8821 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
     8881 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
     8941 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
     9001 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
     9061 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
     9121 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
     9181 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
     9241 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
     9301 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
     9361 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
     9421 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
     9481 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
     9541 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
     9601 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
     9661 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
     9721 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
     9781 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
     9841 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
     9901 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtc
//
