LOCUS       sequence-98294-e         631 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Cre recombinase from the Cbh promoter and one U6-driven 
            sgRNA control targeting LacZ. AAV backbone..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 631)
  AUTHORS   Platt RJ, Chen S, Zhou Y, Yim MJ, Swiech L, Kempton HR, Dahlman JE, 
            Parnas O, Eisenhaure TM, Jovanovic M, Graham DB, Jhunjhunwala S, 
            Heidenreich M, Xavier RJ, Langer R, Anderson DG, Hacohen N, Regev A,
            Feng G, Sharp PA, Zhang F
  TITLE     CRISPR-Cas9 Knockin Mice for Genome Editing and Cancer Modeling.
  JOURNAL   Cell. 2014 Sep 24. pii: S0092-8674(14)01163-5. doi: 
            10.1016/j.cell.2014.09.014.
  PUBMED    25263330
REFERENCE   2  (bases 1 to 631)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2014 
            Sep 24. pii: S0092-8674(14)01163-5. doi: 
            10.1016/j.cell.2014.09.014."
COMMENT     Sequence Label: AAV:ITR-U6-sgRNA(LacZ)-pCBh-Cre-WPRE-hGHpA-ITR
FEATURES             Location/Qualifiers
     source          1..631
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        complement(228..303)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        complement(325..573)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     complement(383..402)
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(553..573)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
ORIGIN
        1 gccaagtggg cagtttaccg taaatactcc acccattgac gtcaatggaa agtccctatt
       61 ggcgttacta ttgacgtcaa tgggcggggg tcgttgggcg gtcagccagg cgggccattt
      121 accgtaagtt atgtaacggg tacctctaga atttgtctgc agaattggcg cacgcgctac
      181 ggactagcct tattttaact tgctatttct agctctaaaa caaaaaagca ccgactcggt
      241 gccacttttt caagttgata acggactagc cttattttaa cttgctattt ctagctctaa
      301 aacatcgcgt gggcgtattc gcacggtgtt tcgtcctttc cacaagatat ataaagccaa
      361 gaaatcgaaa tactttcaag ttacggtaag catatgatag tccattttaa aacataattt
      421 taaaactgca aactacccaa gaaattatta ctttctacgt cacgtatttt gtactaatat
      481 ctttgtgttt acagtcaaat taattccaat tatctctcta acagccttgt atcgtatatg
      541 caaatatgaa ggaatcatgg gaaataggcc ctcacgcgtg cggccgcagg aacccctagt
      601 gatggagttg gccactccct ctctgcgcgc t
//
