LOCUS       sequence-98302-e         838 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Cre recombinase from the Cbh promoter and one U6-driven 
            sgRNA control targeting LacZ. AAV backbone..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 838)
  AUTHORS   Platt RJ, Chen S, Zhou Y, Yim MJ, Swiech L, Kempton HR, Dahlman JE, 
            Parnas O, Eisenhaure TM, Jovanovic M, Graham DB, Jhunjhunwala S, 
            Heidenreich M, Xavier RJ, Langer R, Anderson DG, Hacohen N, Regev A,
            Feng G, Sharp PA, Zhang F
  TITLE     CRISPR-Cas9 Knockin Mice for Genome Editing and Cancer Modeling.
  JOURNAL   Cell. 2014 Sep 24. pii: S0092-8674(14)01163-5. doi: 
            10.1016/j.cell.2014.09.014.
  PUBMED    25263330
REFERENCE   2  (bases 1 to 838)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2014 
            Sep 24. pii: S0092-8674(14)01163-5. doi: 
            10.1016/j.cell.2014.09.014."
COMMENT     Sequence Label: AAV:ITR-U6-sgRNA(LacZ)-pCBh-Cre-WPRE-hGHpA-ITR
FEATURES             Location/Qualifiers
     source          1..838
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             62..82
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 ttaattacct ggagcacctg cctgaaatca ctttttttca ggttggaccg gtgccaccat
       61 gcccaagaag aagaggaagg tgtccaatct cctgactgtt caccagaacc tccctgcgct
      121 gccagtagat gccactagcg atgaggtcag gaaaaatctc atggatatgt ttagggatag
      181 acaggcgttt tctgaacaca cctggaaaat gctgcttagc gtgtgccgat cctgggcagc
      241 ctggtgtaag ctgaacaatc gcaaatggtt ccccgccgag ccggaggacg tgcgcgatta
      301 cctgctgtat ctccaggcaa gagggctggc tgtcaagact atccagcagc acttgggcca
      361 actgaatatg ctgcatcgac gcagcgggct cccccggcct agcgattcaa acgcagtctc
      421 ccttgttatg aggagaatta gaaaggaaaa cgtagatgcg ggtgagaggg ctaagcaggc
      481 tctcgctttt gagcggactg atttcgacca ggtcagatcc ctgatggaga acagcgatcg
      541 gtgccaggac atcaggaacc tcgcatttct gggaattgca tataacacac ttctgcgcat
      601 agctgagatc gcccggatca gagtgaaaga catcagtcga acggacggcg gccggatgct
      661 tattcatatt ggacgcacaa agacattggt cagcaccgct ggcgttgaaa aggccttgtc
      721 cctgggcgta acgaagctgg tggaaagatg gatctcagtg tccggcgtgg ctgacgaccc
      781 taataattac ttgttctgtc gagtgagaaa aaacggagtc gccgcgccct ctgccacc
//
