LOCUS       sequence-98304-e         619 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Cre recombinase from the Cbh promoter and one U6-driven 
            sgRNA targeting the mouse gene NeuN. AAV backbone..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 619)
  AUTHORS   Platt RJ, Chen S, Zhou Y, Yim MJ, Swiech L, Kempton HR, Dahlman JE, 
            Parnas O, Eisenhaure TM, Jovanovic M, Graham DB, Jhunjhunwala S, 
            Heidenreich M, Xavier RJ, Langer R, Anderson DG, Hacohen N, Regev A,
            Feng G, Sharp PA, Zhang F
  TITLE     CRISPR-Cas9 Knockin Mice for Genome Editing and Cancer Modeling.
  JOURNAL   Cell. 2014 Sep 24. pii: S0092-8674(14)01163-5. doi: 
            10.1016/j.cell.2014.09.014.
  PUBMED    25263330
REFERENCE   2  (bases 1 to 619)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2014 
            Sep 24. pii: S0092-8674(14)01163-5. doi: 
            10.1016/j.cell.2014.09.014."
COMMENT     Sequence Label: AAV:ITR-U6-sgRNA(NeuN)-pCBh-Cre-WPRE-hGHpA-ITR
FEATURES             Location/Qualifiers
     source          1..619
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        complement(216..291)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        complement(312..560)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     complement(370..389)
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(540..560)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
ORIGIN
        1 gtttaccgta aatactccac ccattgacgt caatggaaag tccctattgg cgttactatt
       61 gacgtcaatg ggcgggggtc gttgggcggt cagccaggcg ggccatttac cgtaagttat
      121 gtaacgggta cctctagaat ttgtctgcag aattggcgca cgcgctacgg actagcctta
      181 ttttaacttg ctatttctag ctctaaaaca aaaaagcacc gactcggtgc cactttttca
      241 agttgataac ggactagcct tattttaact tgctatttct agctctaaaa ctccggttca
      301 gggaccccga cggtgtttcg tcctttccac aagatatata aagccaagaa atcgaaatac
      361 tttcaagtta cggtaagcat atgatagtcc attttaaaac ataattttaa aactgcaaac
      421 tacccaagaa attattactt tctacgtcac gtattttgta ctaatatctt tgtgtttaca
      481 gtcaaattaa ttccaattat ctctctaaca gccttgtatc gtatatgcaa atatgaagga
      541 atcatgggaa ataggccctc acgcgtgcgg ccgcaggaac ccctagtgat ggagttggcc
      601 actccctctc tgcgcgctc
//
