LOCUS       sequence-98306-e         848 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Cre recombinase from the Cbh promoter and one U6-driven 
            sgRNA targeting the mouse gene NeuN. AAV backbone..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 848)
  AUTHORS   Platt RJ, Chen S, Zhou Y, Yim MJ, Swiech L, Kempton HR, Dahlman JE, 
            Parnas O, Eisenhaure TM, Jovanovic M, Graham DB, Jhunjhunwala S, 
            Heidenreich M, Xavier RJ, Langer R, Anderson DG, Hacohen N, Regev A,
            Feng G, Sharp PA, Zhang F
  TITLE     CRISPR-Cas9 Knockin Mice for Genome Editing and Cancer Modeling.
  JOURNAL   Cell. 2014 Sep 24. pii: S0092-8674(14)01163-5. doi: 
            10.1016/j.cell.2014.09.014.
  PUBMED    25263330
REFERENCE   2  (bases 1 to 848)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2014 
            Sep 24. pii: S0092-8674(14)01163-5. doi: 
            10.1016/j.cell.2014.09.014."
COMMENT     Sequence Label: AAV:ITR-U6-sgRNA(NeuN)-pCBh-Cre-WPRE-hGHpA-ITR
FEATURES             Location/Qualifiers
     source          1..848
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             64..84
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 gtttaattac ctggagcacc tgcctgaaat cacttttttt caggttggac cggtgccacc
       61 atgcccaaga agaagaggaa ggtgtccaat ctcctgactg ttcaccagaa cctccctgcg
      121 ctgccagtag atgccactag cgatgaggtc aggaaaaatc tcatggatat gtttagggat
      181 agacaggcgt tttctgaaca cacctggaaa atgctgctta gcgtgtgccg atcctgggca
      241 gcctggtgta agctgaacaa tcgcaaatgg ttccccgccg agccggagga cgtgcgcgat
      301 tacctgctgt atctccaggc aagagggctg gctgtcaaga ctatccagca gcacttgggc
      361 caactgaata tgctgcatcg acgcagcggg ctcccccggc ctagcgattc aaacgcagtc
      421 tcccttgtta tgaggagaat tagaaaggaa aacgtagatg cgggtgagag ggctaagcag
      481 gctctcgctt ttgagcggac tgatttcgac caggtcagat ccctgatgga gaacagcgat
      541 cggtgccagg acatcaggaa cctcgcattt ctgggaattg catataacac acttctgcgc
      601 atagctgaga tcgcccggat cagagtgaaa gacatcagtc gaacggacgg cggccggatg
      661 cttattcata ttggacgcac aaagacattg gtcagcaccg ctggcgttga aaaggccttg
      721 tccctgggcg taacgaagct ggtggaaaga tggatctcag tgtccggcgt ggctgacgac
      781 cctaataatt acttgttctg tcgagtgaga aaaaacggag tcgccgcgcc ctctgccacc
      841 agccaatt
//
