LOCUS       sequence-98318-e        8699 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mesp driver driving nls::Cas9::nls.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8699)
  AUTHORS   Stolfi A, Gandhi S, Salek F, Christiaen L
  TITLE     Tissue-specific genome editing in Ciona embryos by CRISPR/Cas9.
  JOURNAL   Development. 2014 Nov;141(21):4115-20. doi: 10.1242/dev.114488.
  PUBMED    25336740
REFERENCE   2  (bases 1 to 8699)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1242/dev"; 
            journalName: "Development"; date: "2014-11"; volume: "141"; issue: 
            "21"; pages: "4115-20"
COMMENT     Sequence Label: Mesp-1916/-1>nls::Cas9::nls
FEATURES             Location/Qualifiers
     source          1..8699
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1944..1964
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1965..6065
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             1965..6065
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             6075..6095
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    6425..6559
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6462..6481)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6516..6535
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6595..6614)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6596..6614)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6701..6718)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6872..7460)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6952..6971)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7631..8422,8423..8491))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8254..8273
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8492..8596)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8664..8682
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 ggcgcgcccg gttcaacgtg acgtcccatg ccgatatcgt aaccatccgg aacctctgat
       61 gctttttcaa tatcatcttt tttgaaatcc ttcatttccg tttcatcgtc atttttgaaa
      121 gccgggttct catactcgtt tttggtgctg taaccgtttt ctgacatttt tatctcatcc
      181 aagtgcgaac cacttcaaag atggatagat accagatttt attacaaaca attagcaatt
      241 cacgaaagtt aaaaaaacga cataaaacta taaaataaaa ttatacttat taaatatgaa
      301 gaaaaatatg catttttaat cattctattg caacaaatcg gtatttttcg tattctctta
      361 tacgaagatt gcatacaagc ttaacgtttc atctgtttcc attggaataa aatagaaaac
      421 gtcccaccgc ctgccctaac tttttataag cttcacaaat tatcaaagtt taccgttgaa
      481 ctcctgataa atgattattc agctatttta aacccaactt cgtttataag tgatgccgtc
      541 tccttcttcg tatgaccccg ctctactaaa tacatgtttg cacactgatc ctaatgaaga
      601 ccgcacctga acctttacat agccgatgca ttcacatata atgttctttg tgcaaccgaa
      661 gtcgtatttg agcaaaccaa atatccaact tatataagta gacctttaaa aatggacggt
      721 gaacaaaact ttgcgaagtc atcataattg aaggtttatt tagctctcct attgtaggga
      781 aagatcctat ggatgctcca aaacgataaa cccgacttac cgaaaacgcg gcggtcgtta
      841 gaccatgctt attgataaac cacatacata caaactttag cgcaaactcg gctatatctt
      901 tacaacatta gacaaattct ctttcatgta ttttcatggg attataaagt gtctatttta
      961 ggtaacaaga ctttaataaa aatatcagtg tcccatattg ttggcggctt tgtttgcgag
     1021 tgtctctgta ttgacaacgt ttataattaa cttggaagta atgagtatac agcagcagac
     1081 acagaagttt gtcgggggta tcctatgatt gtacatcatg tgagcaaata tacttcaact
     1141 gctagtgaat aatatttgat atacgcacaa gtcgtacaaa tacacgacta caaataagtc
     1201 atacttgtcg aagtttgtat tcccctgttt tcgatcaatc ttatcagcca caaaaaatgg
     1261 aaaaattcca aaaacgtaga cacccacaaa gtaacataca tggtaactcg taagctaata
     1321 cagagcaccc gtgtgaaaac gactgtcgtt ttccggccac gcgagaataa agtaagttac
     1381 attgattcat tcactgacag tttcccacca aaactgaagt attaatcaat tatgaatcgg
     1441 cagtgctttc agtgggaaga tttcggaaaa tgtgggtcta atgtcacagt aaactttgtg
     1501 tttgttttaa aaacaatcat cgttaaatca acatcgcaat tctgtcaatc actccacaat
     1561 aaactatatg gctaatggaa acaggctgtt tgtttgtata tcataaaata cttgcgtcgt
     1621 attatctgac caaacaaaag cgttacagga acggtccagc ttcaaaattt gctgatgcat
     1681 cagattttac atttgaaatg tgattaatta cgaaaatcca gcgaatagaa ttgtcacaac
     1741 aagtcattag cgacggatat ttcgcctttg aaacttaaag gcgataatga ctttgcccgt
     1801 ttcatgcggc gataaacgaa ctaattagac acctcctaca gatataatgg taattcagaa
     1861 tcgtgtggtt atgtaattcg caaaaacatt ttaacaaaac aggttgattt gaaacttgta
     1921 ttgcggccgc aaccatggct agccccaaaa agaagaggaa agtggacaag aagtattcta
     1981 tcggactgga catcgggact aatagcgtcg ggtgggccgt gatcactgac gagtacaagg
     2041 tgccctctaa gaagttcaag gtgctcggga acaccgaccg gcattccatc aagaaaaatc
     2101 tgatcggagc tctcctcttt gattcagggg agaccgctga agcaacccgc ctcaagcgga
     2161 ctgctagacg gcggtacacc aggaggaaga accggatttg ttaccttcaa gagatattct
     2221 ccaacgaaat ggcaaaggtc gacgacagct tcttccatag gctggaagaa tcattcctcg
     2281 tggaagagga taagaagcat gaacggcatc ccatcttcgg taatatcgtc gacgaggtgg
     2341 cctatcacga gaaataccca accatctacc atcttcgcaa aaagctggtg gactcaaccg
     2401 acaaggcaga cctccggctt atctacctgg ccctggccca catgatcaag ttcagaggcc
     2461 acttcctgat cgagggcgac ctcaatcctg acaatagcga tgtggataaa ctgttcatcc
     2521 agctggtgca gacttacaac cagctctttg aagagaaccc catcaatgca agcggagtcg
     2581 atgccaaggc cattctgtca gcccggctgt caaagagccg cagacttgag aatcttatcg
     2641 ctcagctgcc gggtgaaaag aaaaatggac tgttcgggaa cctgattgct ctttcacttg
     2701 ggctgactcc caatttcaag tctaatttcg acctggcaga ggatgccaag ctgcaactgt
     2761 ccaaggacac ctatgatgac gatctcgaca acctcctggc ccagatcggt gaccaatacg
     2821 ccgacctttt ccttgctgct aagaatcttt ctgacgccat cctgctgtct gacattctcc
     2881 gcgtgaacac tgaaatcacc aaggcccctc tttcagcttc aatgattaag cggtatgatg
     2941 agcaccacca ggacctgacc ctgcttaagg cactcgtccg gcagcagctt ccggagaagt
     3001 acaaggaaat cttctttgac cagtcaaaga atggatacgc cggctacatc gacggaggtg
     3061 cctcccaaga ggaattttat aagtttatca aacctatcct tgagaagatg gacggcaccg
     3121 aagagctcct cgtgaaactg aatcgggagg atctgctgcg gaagcagcgc actttcgaca
     3181 atgggagcat tccccaccag atccatcttg gggagcttca cgccatcctt cggcgccaag
     3241 aggacttcta cccctttctt aaggacaaca gggagaagat tgagaaaatt ctcactttcc
     3301 gcatccccta ctacgtggga cccctcgcca gaggaaatag ccggtttgct tggatgacca
     3361 gaaagtcaga agaaactatc actccctgga acttcgaaga ggtggtggac aagggagcca
     3421 gcgctcagtc attcatcgaa cggatgacta acttcgataa gaacctcccc aatgagaagg
     3481 tcctgccgaa acattccctg ctctacgagt actttaccgt gtacaacgag ctgaccaagg
     3541 tgaaatatgt caccgaaggg atgaggaagc ccgcattcct gtcaggcgaa caaaagaagg
     3601 caattgtgga ccttctgttc aagaccaata gaaaggtgac cgtgaagcag ctgaaggagg
     3661 actatttcaa gaaaattgaa tgcttcgact ctgtggagat tagcggggtc gaagatcggt
     3721 tcaacgcaag cctgggtacc taccatgatc tgcttaagat catcaaggac aaggattttc
     3781 tggacaatga ggagaacgag gacatccttg aggacattgt cctgactctc actctgttcg
     3841 aggaccggga aatgatcgag gagaggctta agacctacgc ccatctgttc gacgataaag
     3901 tgatgaagca acttaaacgg agaagatata ccggatgggg acgccttagc cgcaaactca
     3961 tcaacggaat ccgggacaaa cagagcggaa agaccattct tgatttcctt aagagcgacg
     4021 gattcgctaa tcgcaacttc atgcaactta tccatgatga ttccctgacc tttaaggagg
     4081 acatccagaa ggcccaagtg tctggacaag gtgactcact gcacgagcat atcgcaaatc
     4141 tggctggttc acccgctatt aagaagggta ttctccagac cgtgaaagtc gtggacgagc
     4201 tggtcaaggt gatgggtcgc cataaaccag agaacattgt catcgagatg gccagggaaa
     4261 accagactac ccagaaggga cagaagaaca gcagggagcg gatgaaaaga attgaggaag
     4321 ggattaagga gctcgggtca cagatcctta aagagcaccc ggtggaaaac acccagcttc
     4381 agaatgagaa gctctatctg tactaccttc aaaatggacg cgatatgtat gtggaccaag
     4441 agcttgatat caacaggctc tcagactacg acgtggacca catcgtccct cagagcttcc
     4501 tcaaagacga ctcaattgac aataaggtgc tgactcgctc agacaagaac cggggaaagt
     4561 cagataacgt gccctcagag gaagtcgtga aaaagatgaa gaactattgg cgccagcttc
     4621 tgaacgcaaa gctgatcact cagcggaagt tcgacaatct cactaaggct gagaggggcg
     4681 gactgagcga actggacaaa gcaggattca ttaaacggca acttgtggag actcggcaga
     4741 ttactaaaca tgtcgcccaa atccttgact cacgcatgaa taccaagtac gacgaaaacg
     4801 acaaacttat ccgcgaggtg aaggtgatta ccctgaagtc caagctggtc agcgatttca
     4861 gaaaggactt tcaattctac aaagtgcggg agatcaataa ctatcatcat gctcatgacg
     4921 catatctgaa tgccgtggtg ggaaccgccc tgatcaagaa gtacccaaag ctggaaagcg
     4981 agttcgtgta cggagactac aaggtctacg acgtgcgcaa gatgattgcc aaatctgagc
     5041 aggagatcgg aaaggccacc gcaaagtact tcttctacag caacatcatg aatttcttca
     5101 agaccgaaat cacccttgca aacggtgaga tccggaagag gccgctcatc gagactaatg
     5161 gggagactgg cgaaatcgtg tgggacaagg gcagagattt cgctaccgtg cgcaaagtgc
     5221 tttctatgcc tcaagtgaac atcgtgaaga aaaccgaggt gcaaaccgga ggcttttcta
     5281 aggaatcaat cctccccaag cgcaactccg acaagctcat tgcaaggaag aaggattggg
     5341 accctaagaa gtacggcgga ttcgattcac caactgtggc ttattctgtc ctggtcgtgg
     5401 ctaaggtgga aaaaggaaag tctaagaagc tcaagagcgt gaaggaactg ctgggtatca
     5461 ccattatgga gcgcagctcc ttcgagaaga acccaattga ctttctcgaa gccaaaggtt
     5521 acaaggaagt caagaaggac cttatcatca agctcccaaa gtatagcctg ttcgaactgg
     5581 agaatgggcg gaagcggatg ctcgcctccg ctggcgaact tcagaagggt aatgagctgg
     5641 ctctcccctc caagtacgtg aatttcctct accttgcaag ccattacgag aagctgaagg
     5701 ggagccccga ggacaacgag caaaagcaac tgtttgtgga gcagcataag cattatctgg
     5761 acgagatcat tgagcagatt tccgagtttt ctaaacgcgt cattctcgct gatgccaacc
     5821 tcgataaagt ccttagcgca tacaataagc acagagacaa accaattcgg gagcaggctg
     5881 agaatatcat ccacctgttc accctcacca atcttggtgc ccctgccgca ttcaagtact
     5941 tcgacaccac catcgaccgg aaacgctata cctccaccaa agaagtgctg gacgccaccc
     6001 tcatccacca gagcatcacc ggactttacg aaactcggat tgacctctca cagctcggag
     6061 gggatgaggg agctcccaag aaaaagcgca aggtaggtta atgagaattc cagctgagcg
     6121 ccggtcgcta ccattaccag ttggtctggt gtcaaaaata ataataaccg ggcaggccat
     6181 gtctgcccgt atttcgcgta aggaaatcca ttatgtacta tttaaaaaac acaaactttt
     6241 ggatgttcgg tttattcttt ttcttttact tttttatcat gggagcctac ttcccgtttt
     6301 tcccgatttg gctacatgac atcaaccata tcagcaaaag tgatacgggt attatttttg
     6361 ccgctatttc tctgttctcg ctattattcc aaccgctgtt tggtctgctt tctgacaaac
     6421 tcggaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
     6481 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
     6541 atcttatcat gtctggatcg acaaagtcaa agcggccatc agatctgccg gtctccctat
     6601 agtgagtcgt attaatttcg ataagccagg ttaacctgca ttaatgaatc ggccaacgcg
     6661 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc
     6721 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
     6781 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
     6841 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     6901 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     6961 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     7021 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcaatgc tcacgctgta
     7081 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     7141 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     7201 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     7261 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat
     7321 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     7381 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     7441 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
     7501 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
     7561 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
     7621 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
     7681 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
     7741 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
     7801 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
     7861 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
     7921 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
     7981 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
     8041 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
     8101 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
     8161 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
     8221 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
     8281 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
     8341 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
     8401 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
     8461 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
     8521 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
     8581 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa gaaaccatta
     8641 ttatcatgac attaacctat aaaaataggc gtatcacgag gcccttacgt attaattaa
//
