LOCUS       sequence-98319-e        9163 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Sox1/2/3 (SoxB1) driver driving nls::Cas9::nls.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9163)
  AUTHORS   Stolfi A, Gandhi S, Salek F, Christiaen L
  TITLE     Tissue-specific genome editing in Ciona embryos by CRISPR/Cas9.
  JOURNAL   Development. 2014 Nov;141(21):4115-20. doi: 10.1242/dev.114488.
  PUBMED    25336740
REFERENCE   2  (bases 1 to 9163)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1242/dev"; 
            journalName: "Development"; date: "2014-11"; volume: "141"; issue: 
            "21"; pages: "4115-20"
COMMENT     Sequence Label: Sox1/2/3-2373/+3>nls::Cas9::nls
FEATURES             Location/Qualifiers
     source          1..9163
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             2408..2428
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2429..6529
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             2429..6529
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             6539..6559
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    6889..7023
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6926..6945)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6980..6999
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7059..7078)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7060..7078)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7165..7182)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7336..7924)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7416..7435)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8095..8886,8887..8955))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8718..8737
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8956..9060)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9128..9146
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 ggcgcgcccg ctcgcatgtc aaatagttcg aattttattg tagaactcgg gcagttgtga
       61 tgtaacacgt gaattggatt cagcattcgt tctttaaacc tatgagtcgg caaggaagcg
      121 atctgtcaaa ttaaatcgaa tctttcagcg ctcgcatcga gacatagcga tactggaagc
      181 gtcggaaata gaatagtggg gttgatgaca ggtgaaaaca gaattcgtgg cgcaaattac
      241 gaaatatttt tgcgctggtt ttattaaaca acaattttct caatagtggg gtcgaaattg
      301 atgtttgcat tttgttgtag gggttgtaca tgcgggatac gctccgtggt aggcgtctat
      361 gttatacagt cggtgtaact taacgacaaa tatagaatac gttgtgttcg ttcattgatg
      421 gtcttgagtt gcacgatttc ccgtcgcaaa tgaaccgtta aacacaactt ggcccgaagc
      481 aggcatggca gaagtcgtcg taagtaggct ggggtgtcgt ggggccgcgc gtcgtcatgc
      541 taaaaccgac cgagcagagc gtaggcaaaa gtaagcttcg aattgagtaa aacgcgacga
      601 ataaaacggt ggtacttgtc ggtttgtccg cgctgaaacc taataactgg ctacgcgtgt
      661 tttctaggat gtcgagtgtt cgcggacact tgaacactta gatatctaca tgctgtcgtt
      721 gctttggttg gttttacact ggatttgtat tgctggtgtt ttgctgtaaa gccagagttt
      781 gtaaccatat agacatcgta tatttgtaat gtagtattcc tagttttgag tatagagctc
      841 gctatttagt tctgttgtca gttttaagtg tgttaaggca agatacataa agaaacaaag
      901 tctttatcta tcttgtcgaa tagagtaacg aagatgaaat tattaaaact ttaaaaacaa
      961 ggaattaaca gcaaaatgac agttggtaaa acacgctatg aataaactgt attgaaaaac
     1021 atcaggaaat cgtttggttt gtgttcaatt tgtttaattt agaagttttg tattgttagt
     1081 ataatgactg ttgtctgtac acaacacgtg ttgaccacta tagtagtgta gtagcccaat
     1141 aacaattact gatgttctgc gcataagtcg cgtgtgcttt actgggcatt catgccggta
     1201 gaattaacca ttcatcgtaa aagaacaaaa cggtctattc tgatccgttc attgagtggc
     1261 ttatgaattg gcaaaagtgc ttgtaaagtc ggcactgaat gagcgctgcc tttgttactg
     1321 acaatgtgca ttcatgacga tagttccgaa aggtgtggaa gtaaaataca ctgttcgtgt
     1381 tcatactacg cattccgagg gggaatacct ttttgatacg ccatgtatgc ttgatttttt
     1441 ttgacaaaaa taaacattac aattattgga acgacacttt gttgatattt ttgtatgggt
     1501 gttatacgtt aatggggatg ttattgaaat atagagttgt gtaattgtaa ttgtgcggtt
     1561 tcatgttaac gcttaaaaca gttttctttc taagctggtg ttttgagttt taggtatata
     1621 tgctaaaggt ttaagtattg ggttatgagt tttgttattc gtttcttttt tgtaaatata
     1681 acttttgcgg gttttcatat ttttcgattc gtataaaatc gaattctgtc ttatcatgaa
     1741 ccgaccacgt ttttcgctga ttcgaagtcg tttagatggt tgctttaggg aacgctggat
     1801 cccaacgaaa agaaaaaggc gacgttcgcc tccgcgaaga taagtcgtag cgagagaaag
     1861 gtcgtttgta gcgcaataaa ggcagcctgt gagatgccta cttcattcat tctgcctttt
     1921 gtgacttcat agtggcattg tgagcttcta ttcaggcatt gtctccaaga aagttatgag
     1981 tctatagatt ataagacctc ttctatggac aagcagaaca attgaattac aattgttaaa
     2041 aacaatcgta aattgtagac cgtagtttca atagaattaa cgcgagaata tttctggagt
     2101 aaaaagactg aaatagaaaa atagggacca ttaaaaattg tcagccgata gccagaatat
     2161 tttacaagtc aactgtaatt tgtgtttgaa gttttttaaa ttgatttttt taactgagac
     2221 ttctacttta cagcgtttgg aatcaagtaa agatatttaa ctcaattctt gcgaacattc
     2281 gcctaaagtc tcacacgtca ttaaactgga ttttgtagct tacaaaaact tctccgtccc
     2341 tactccaccg gggtttctga aagagccatc tcagaacgac ttcatggcgg ccgcaaccat
     2401 ggctagcccc aaaaagaaga ggaaagtgga caagaagtat tctatcggac tggacatcgg
     2461 gactaatagc gtcgggtggg ccgtgatcac tgacgagtac aaggtgccct ctaagaagtt
     2521 caaggtgctc gggaacaccg accggcattc catcaagaaa aatctgatcg gagctctcct
     2581 ctttgattca ggggagaccg ctgaagcaac ccgcctcaag cggactgcta gacggcggta
     2641 caccaggagg aagaaccgga tttgttacct tcaagagata ttctccaacg aaatggcaaa
     2701 ggtcgacgac agcttcttcc ataggctgga agaatcattc ctcgtggaag aggataagaa
     2761 gcatgaacgg catcccatct tcggtaatat cgtcgacgag gtggcctatc acgagaaata
     2821 cccaaccatc taccatcttc gcaaaaagct ggtggactca accgacaagg cagacctccg
     2881 gcttatctac ctggccctgg cccacatgat caagttcaga ggccacttcc tgatcgaggg
     2941 cgacctcaat cctgacaata gcgatgtgga taaactgttc atccagctgg tgcagactta
     3001 caaccagctc tttgaagaga accccatcaa tgcaagcgga gtcgatgcca aggccattct
     3061 gtcagcccgg ctgtcaaaga gccgcagact tgagaatctt atcgctcagc tgccgggtga
     3121 aaagaaaaat ggactgttcg ggaacctgat tgctctttca cttgggctga ctcccaattt
     3181 caagtctaat ttcgacctgg cagaggatgc caagctgcaa ctgtccaagg acacctatga
     3241 tgacgatctc gacaacctcc tggcccagat cggtgaccaa tacgccgacc ttttccttgc
     3301 tgctaagaat ctttctgacg ccatcctgct gtctgacatt ctccgcgtga acactgaaat
     3361 caccaaggcc cctctttcag cttcaatgat taagcggtat gatgagcacc accaggacct
     3421 gaccctgctt aaggcactcg tccggcagca gcttccggag aagtacaagg aaatcttctt
     3481 tgaccagtca aagaatggat acgccggcta catcgacgga ggtgcctccc aagaggaatt
     3541 ttataagttt atcaaaccta tccttgagaa gatggacggc accgaagagc tcctcgtgaa
     3601 actgaatcgg gaggatctgc tgcggaagca gcgcactttc gacaatggga gcattcccca
     3661 ccagatccat cttggggagc ttcacgccat ccttcggcgc caagaggact tctacccctt
     3721 tcttaaggac aacagggaga agattgagaa aattctcact ttccgcatcc cctactacgt
     3781 gggacccctc gccagaggaa atagccggtt tgcttggatg accagaaagt cagaagaaac
     3841 tatcactccc tggaacttcg aagaggtggt ggacaaggga gccagcgctc agtcattcat
     3901 cgaacggatg actaacttcg ataagaacct ccccaatgag aaggtcctgc cgaaacattc
     3961 cctgctctac gagtacttta ccgtgtacaa cgagctgacc aaggtgaaat atgtcaccga
     4021 agggatgagg aagcccgcat tcctgtcagg cgaacaaaag aaggcaattg tggaccttct
     4081 gttcaagacc aatagaaagg tgaccgtgaa gcagctgaag gaggactatt tcaagaaaat
     4141 tgaatgcttc gactctgtgg agattagcgg ggtcgaagat cggttcaacg caagcctggg
     4201 tacctaccat gatctgctta agatcatcaa ggacaaggat tttctggaca atgaggagaa
     4261 cgaggacatc cttgaggaca ttgtcctgac tctcactctg ttcgaggacc gggaaatgat
     4321 cgaggagagg cttaagacct acgcccatct gttcgacgat aaagtgatga agcaacttaa
     4381 acggagaaga tataccggat ggggacgcct tagccgcaaa ctcatcaacg gaatccggga
     4441 caaacagagc ggaaagacca ttcttgattt ccttaagagc gacggattcg ctaatcgcaa
     4501 cttcatgcaa cttatccatg atgattccct gacctttaag gaggacatcc agaaggccca
     4561 agtgtctgga caaggtgact cactgcacga gcatatcgca aatctggctg gttcacccgc
     4621 tattaagaag ggtattctcc agaccgtgaa agtcgtggac gagctggtca aggtgatggg
     4681 tcgccataaa ccagagaaca ttgtcatcga gatggccagg gaaaaccaga ctacccagaa
     4741 gggacagaag aacagcaggg agcggatgaa aagaattgag gaagggatta aggagctcgg
     4801 gtcacagatc cttaaagagc acccggtgga aaacacccag cttcagaatg agaagctcta
     4861 tctgtactac cttcaaaatg gacgcgatat gtatgtggac caagagcttg atatcaacag
     4921 gctctcagac tacgacgtgg accacatcgt ccctcagagc ttcctcaaag acgactcaat
     4981 tgacaataag gtgctgactc gctcagacaa gaaccgggga aagtcagata acgtgccctc
     5041 agaggaagtc gtgaaaaaga tgaagaacta ttggcgccag cttctgaacg caaagctgat
     5101 cactcagcgg aagttcgaca atctcactaa ggctgagagg ggcggactga gcgaactgga
     5161 caaagcagga ttcattaaac ggcaacttgt ggagactcgg cagattacta aacatgtcgc
     5221 ccaaatcctt gactcacgca tgaataccaa gtacgacgaa aacgacaaac ttatccgcga
     5281 ggtgaaggtg attaccctga agtccaagct ggtcagcgat ttcagaaagg actttcaatt
     5341 ctacaaagtg cgggagatca ataactatca tcatgctcat gacgcatatc tgaatgccgt
     5401 ggtgggaacc gccctgatca agaagtaccc aaagctggaa agcgagttcg tgtacggaga
     5461 ctacaaggtc tacgacgtgc gcaagatgat tgccaaatct gagcaggaga tcggaaaggc
     5521 caccgcaaag tacttcttct acagcaacat catgaatttc ttcaagaccg aaatcaccct
     5581 tgcaaacggt gagatccgga agaggccgct catcgagact aatggggaga ctggcgaaat
     5641 cgtgtgggac aagggcagag atttcgctac cgtgcgcaaa gtgctttcta tgcctcaagt
     5701 gaacatcgtg aagaaaaccg aggtgcaaac cggaggcttt tctaaggaat caatcctccc
     5761 caagcgcaac tccgacaagc tcattgcaag gaagaaggat tgggacccta agaagtacgg
     5821 cggattcgat tcaccaactg tggcttattc tgtcctggtc gtggctaagg tggaaaaagg
     5881 aaagtctaag aagctcaaga gcgtgaagga actgctgggt atcaccatta tggagcgcag
     5941 ctccttcgag aagaacccaa ttgactttct cgaagccaaa ggttacaagg aagtcaagaa
     6001 ggaccttatc atcaagctcc caaagtatag cctgttcgaa ctggagaatg ggcggaagcg
     6061 gatgctcgcc tccgctggcg aacttcagaa gggtaatgag ctggctctcc cctccaagta
     6121 cgtgaatttc ctctaccttg caagccatta cgagaagctg aaggggagcc ccgaggacaa
     6181 cgagcaaaag caactgtttg tggagcagca taagcattat ctggacgaga tcattgagca
     6241 gatttccgag ttttctaaac gcgtcattct cgctgatgcc aacctcgata aagtccttag
     6301 cgcatacaat aagcacagag acaaaccaat tcgggagcag gctgagaata tcatccacct
     6361 gttcaccctc accaatcttg gtgcccctgc cgcattcaag tacttcgaca ccaccatcga
     6421 ccggaaacgc tatacctcca ccaaagaagt gctggacgcc accctcatcc accagagcat
     6481 caccggactt tacgaaactc ggattgacct ctcacagctc ggaggggatg agggagctcc
     6541 caagaaaaag cgcaaggtag gttaatgaga attccagctg agcgccggtc gctaccatta
     6601 ccagttggtc tggtgtcaaa aataataata accgggcagg ccatgtctgc ccgtatttcg
     6661 cgtaaggaaa tccattatgt actatttaaa aaacacaaac ttttggatgt tcggtttatt
     6721 ctttttcttt tactttttta tcatgggagc ctacttcccg tttttcccga tttggctaca
     6781 tgacatcaac catatcagca aaagtgatac gggtattatt tttgccgcta tttctctgtt
     6841 ctcgctatta ttccaaccgc tgtttggtct gctttctgac aaactcggaa cttgtttatt
     6901 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
     6961 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg
     7021 atcgacaaag tcaaagcggc catcagatct gccggtctcc ctatagtgag tcgtattaat
     7081 ttcgataagc caggttaacc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
     7141 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
     7201 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
     7261 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     7321 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     7381 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     7441 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     7501 tctcccttcg ggaagcgtgg cgctttctca atgctcacgc tgtaggtatc tcagttcggt
     7561 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     7621 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     7681 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     7741 cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct
     7801 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     7861 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     7921 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     7981 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
     8041 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
     8101 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
     8161 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
     8221 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
     8281 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
     8341 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
     8401 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
     8461 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
     8521 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
     8581 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
     8641 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
     8701 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
     8761 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
     8821 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
     8881 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
     8941 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
     9001 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
     9061 cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac
     9121 ctataaaaat aggcgtatca cgaggccctt acgtattaat taa
//
