LOCUS       sequence-9833-ex       12807 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12807)
  AUTHORS   Kita-Matsuo H, Barcova M, Prigozhina N, Salomonis N, Wei K, Jacot 
            JG, Nelson B, Spiering S, Haverslag R, Kim C, Talantova M, Bajpai R,
            Calzolari D, Terskikh A, McCulloch AD, Price JH, Conklin BR, Chen 
            HS, Mercola M
  TITLE     Lentiviral vectors and protocols for creation of stable hESC lines 
            for fluorescent tracking and drug resistance selection of 
            cardiomyocytes.
  JOURNAL   PLoS ONE. 2009  . 4(4):e5046.
  PUBMED    19352491
REFERENCE   2  (bases 1 to 12807)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS ONE. 
            2009  . 4(4):e5046."
COMMENT     Sequence Label: aMHC-mCherry-Rex-Blasticidin
FEATURES             Location/Qualifiers
     source          1..12807
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        50..353
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        354..557
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     508..528
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             571..751
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    798..923
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    973..1089
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     primer_bind     1165..1181
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1165..1181
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     CDS             6639..7349
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(6784..6802)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     7052..7071
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             8089..8487
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    8493..9081
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8546..8566)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8964..8975)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     misc_feature    9091..9324
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     protein_bind    complement(9440..9473)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     LTR             9544..9724
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(9774..9793)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(10007..10026)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     10126..10148
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(10186..10204)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        10272..10376
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10377..10445,10446..11237)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10595..10614)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11408..11996
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11897..11916
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     12150..12167
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(12237..12257)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        12255..12552
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     promoter        12353..12563
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      12422..12557
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    12673..12807
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     12764..12783
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 gggccccaat tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac
       61 ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa
      121 tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt
      181 atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc
      241 ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat
      301 gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc
      361 ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga tttccaagtc
      421 tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa
      481 aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta cggtgggagg
      541 tctatataag cagagctggt ttagtgaacc gggtctctct ggttagacca gatctgagcc
      601 tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga
      661 gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga
      721 cccttttagt cagtgtggaa aatctctagc agtggcgccc gaacagggac ctgaaagcga
      781 aagggaaacc agaggagctc tctcgacgca ggactcggct tgctgaagcg cgcacggcaa
      841 gaggcgaggg gcggcgactg cagagtacgc caaaaatttt gactagcgga ggctagaagg
      901 agagagatgg gtgcgagagc gtcagtatta agcgggggaa aataagcccg ggatggcagt
      961 attcatccac aattttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat
     1021 agtagacata atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat
     1081 tcaaattttc gggtttatta cagggacagc ggcctatggg aatgaaagac cccacctgtc
     1141 gagctccacc gcggtggcgg ccgctctaga actagtggat cctgcaaggt cacacaaggg
     1201 tctccaccca ccaggtgccc tagtctcaat ttcagtttcc atgccttgtt ctcacaatgc
     1261 tggcctcccc agagctaatt tggactttgt ttttatttca aaagggcctg aatgaggagt
     1321 agatcttgtg ctacccagct ctaagggtgc ccgtgaagcc ctcagacctg gagcctttgc
     1381 aacagccctt taggtggaag cagaataaag caattttcct taaagccaaa atcctgcctc
     1441 tagactcttc ttctctgacc tcggtccctg ggctctaggg tggggaggtg gggcttggaa
     1501 gaagaaggtg gggaagtggc aaaagccgat ccctagggcc ctgtgaagtt cggagccttc
     1561 cctgtacagc actggctcat agatcctcct ccagccaaac atagcaagaa gtgatacctc
     1621 ctttgtgact tccccaggcc cagtacctgt caggttgaaa caggatttag agaagcctct
     1681 gaactcacct gaactctgaa gctcatccac caagcaagca cctaggtgcc actgctagtt
     1741 agtatcctac gctgataata tgcagagctg ggccacagaa gtcctggggt gtaggaactg
     1801 accagtgact tttcagtcgg caaaggtatg accccctcag cagatgtagt aatgtcccct
     1861 tagatcccat cccaggcagg tctctaagag gacatgggat gagagatgta gtcatgtggc
     1921 attccaaaca cagctatcca cagtgtccct tgccccttcc acttagccag gaggacagta
     1981 accttagcct atctttcttc ctccccatcc tcccaggaca caccccctgg tctgcagtat
     2041 tcatttcttc cttcacgtcc cctctgtgac ttccatttgc aaggcttttg acctctgcag
     2101 ctgctggaag atagagtttg gccctaggtg tggcaagcca tctcaagaga aagcagacaa
     2161 cagggggacc agattttgga aggatcagga actaaatcac tggcgggcct gggggtagaa
     2221 aaaagagtga gtgagtccgc tccagctaag ccaagctagt ccccgagata ctctgccaca
     2281 gctgggctgc tcggggtagc tttaggaatg tgggtctgaa agacaatggg attggaagac
     2341 atctctttga gtctcccctc aaccccacct acagacacac tcgtgtgtgg ccagactcct
     2401 gttcaacagc cctctgtgtt ctgaccactg agctaggcaa ccagagcatg ggccctgtgc
     2461 tgaggatgaa gagttggtta ccaatagcaa aaacagcagg ggagggagaa cagagaacga
     2521 aataaggaag gaagaaggaa aggccagtca atcagatgca gtcagaagag atgggaagcc
     2581 aacacacagc ttgagcagag gaaacagaaa agggagagat tctgggcata aggaggccac
     2641 agaaagaaga gcccaggccc cccaagtctc ctctttatac cctcatcccg tctcccaatt
     2701 aagcccactc ttcttcctag atcagacctg agctgcagcg aagagacccg tagggaggat
     2761 cacactggat gaaggagatg tgtggagaag tccagggaac ctaagagcca gagcctaaaa
     2821 gagcaagaga taaaggtgct tcaaaggtgg ccaggctgtg cacacagagg gtcgaggact
     2881 ggtggtagag cctcaagata aggatgatgc tcagaatggg cggggggggg gattctgggg
     2941 gggggagaga gaaggtgaga aggagcctgg aacagagaat ctggaagcgc tggaaacgat
     3001 accataaagg gaagaaccca ggctaccttt agatgtaaat catgaaagac agggagaagg
     3061 gaagctggag agagtagaag gaccccgggg caagacatgg aagcaaggac aagccaggtt
     3121 gagcgctccg tgaaatcagc ctgctgaagg cagagccctg gtatgagcac cagaacagca
     3181 gaggctaggg ttaatgtcga gacagggaac agaaggtaga cacaggaaca gacagagacg
     3241 ggggagccag gtaacaaagg aatggtcctt ctcacctgtg gccagagcgt ccatctgtgt
     3301 ccacatactc tagaatgttc atcagactgc agggctggct tgggaggcag ctggaaagag
     3361 tatgtgagag ccaggggaga caagggggcc taggaaagga agaagagggc aaaccaggcc
     3421 acacaagagg gcagagccca gaactgagtt aactccttcc ttgttgcatc ttccatagga
     3481 ggcagtggga actctgtgac caccatcccc catgagcccc cactacccat accaagtttg
     3541 gcctgagtgg cattctaggt tccctgagga cagagcctgg cctttgtctc ttggacctga
     3601 cccaagctga cccaatgttc tcagtacctt atcatgccct caagagcttg agaaccaggc
     3661 agtgacatat taggccatgg gctaaccctg gagcttgcac acaggagcct caagtgacct
     3721 ccagggacac agctgcagac aggtggcctt tatccccaaa gagcaaccat ttggcatagg
     3781 tggctgcaaa tgggaatgca aggttgaatc aggtcccttc aagaatactg catgcaagac
     3841 ctaagacccc tggagagagg ggtatgctcc tgcccccacc caccataagg ggagtgaact
     3901 atcctagggg gctggcgacc ttggggagac accacattac tgagagtgct gagcccagaa
     3961 aaactgaccg ccctgtgtcc tgcccacctc cacactctag agctatattg agaggtgaca
     4021 gtagataggg tgggagctgg tagcagggag agtgttcctg ggtgtgaggg tgtaggggaa
     4081 agccagagca ggggagtctg gctttgtctc ctgaacacaa tgtctactta gttataacag
     4141 gcatgacctg ctaaagaccc aacatctacg acctctgaaa agacagcagc cctggaggac
     4201 aggggttgtc tctgagcctt gggtgcttga tggtgccaca aaggagggca tgagtgtgag
     4261 tataaggccc caggagcgtt agagaagggc acttgggaag gggtcagtct gcagagcccc
     4321 tatccatgga atctggagcc tggggccaac tggtgtaaat ctctgggcct gccaggcatt
     4381 caaagcagca cctgcatcct ctggcagcct ggggaggcgg aagggagcaa ccccccactt
     4441 ataccctttc tccctcagcc ccaggattaa cacctctggc cttccccctt cccacctccc
     4501 atcaggagtg gagggttgca gagggagggt aaaaacctac atgtccaaac atcatggtgc
     4561 acgatatatg gatcagtatg tgtagaggca agaaaggaaa tctgcaggct taactgggtt
     4621 aatgtgtaaa gtctgtgtgc atgtgtgtgt gtctgactga aaacgggcat ggctgtgcag
     4681 ctgttcagtt ctgtgcgtga ggttaccaga ctgcaggttt gtgtgtaaat tgcccaaggc
     4741 aaagtgggtg aatcccttcc atggtttaaa gagattggat gatggcctgc atctcaagga
     4801 ccatggaaaa tagaatggac actctatatg tgtctctaag ctaaggtagc aaggtctttg
     4861 gaggacacct gtctagagat gtgggcaaca gagactacag acagtatctg tacagagtaa
     4921 ggagagagag gagggggtgt agaattctct tactatcaaa gggaaactga gtcgtgcacc
     4981 tgcaaagtgg atgctctccc tagacatcat gactttgtct ctggggagcc agcactgtgg
     5041 aacttcaggt ctgagagagt aggaggctcc cctcagcctg aagctatgca gatagccagg
     5101 gttgaaaggg ggaagggaga gcctgggatg ggagcttgtg tgttggaggc aggggacaga
     5161 tattaagcct ggaagagaag gtgaccctta cccagttgtt caactcaccc ttcagattaa
     5221 aaataactga ggtaagggcc tgggtagggg aggtggtgtg agacgctcct gtctctcctc
     5281 tatctgccca tcggcccttt ggggaggagg aatgtgccca aggactaaaa aaaggccatg
     5341 gagccagagg ggcgagggca acagaccttt catgggcaaa ccttggggcc ctgctgtcct
     5401 cctgtcacct ccagagccaa gggatcaaag gaggaggagc caggacagga gggaagtggg
     5461 agggagggtc ccagcagagg actccaaatt taggcagcag gcatatggga tgggatataa
     5521 aggggctgga gcactgagag ctgtcagaga tttctccaac ccaggtaaga gggagtttcg
     5581 ggtgggggct cttcacccac accagacctc tccccaccta gaaggaaact gcctttcctg
     5641 gaagtggggt tcaggccggt cagagatctg acagggtggc cttccaccag cctgggaagt
     5701 tctcagtggc aggaggtttc cacaagaaac actggatgcc ccttccctta cgctgtcttc
     5761 tccatcttcc tcctggggat gctcctcccc gtcttggttt atcttggctc ttcgtcttca
     5821 gcaagatttg ccctgtgctg tccactccat ctttctctac tgtctccgtg ccttgccttg
     5881 ccttcttgcg tgtccttcct ttccacccat ttctcacttc accttttctc cccttctcat
     5941 ttgtattcat ccttccttcc ttccttcctt ccttccttcc ttccttcctt ccttcctttc
     6001 tcccttcctt ccttccttcc ttccttcctt ccttccttcc ttcctgtgtc agagtgctga
     6061 gaatcacacc tggggttccc acccttatgt aaacaatctt ccagtgagcc acagcttcag
     6121 tgctgctggg tgctctctta ccttcctcac cccctggctt gtcctgttcc atcctggtca
     6181 ggatctctag attggtctcc cagcctctgc tactcctctt cctgcctgtt cctctctctg
     6241 tccagctgcg ccactgtggt gcctcgttcc agctgtggtc cacattcttc aggattctct
     6301 gaaaagttaa ccaggtgaga atgtttcccc tgtagacagc agatcacgat tctcccggaa
     6361 gtcaggcttc cagccctctc tttctctgcc cagctgcccg gcactcttag caaacctcag
     6421 gcacccttac cccacataga cctctgacag agaagcaggc actttacatg gagtcctggt
     6481 gggagagcca taggctacgg tgtaaaagag gcagggaagt ggtggtgtag gaaagtcagg
     6541 acttcacata gaagcctagc ccacaccaga aatgacagac agatccctcc tatctccccc
     6601 ataagagttt gagtcgactg atcaaaaacc ggtccaccat ggtgagcaag ggcgaggagg
     6661 ataacatggc catcatcaag gagttcatgc gcttcaaggt gcacatggag ggctccgtga
     6721 acggccacga gttcgagatc gagggcgagg gcgagggccg cccctacgag ggcacccaga
     6781 ccgccaagct gaaggtgacc aagggtggcc ccctgccctt cgcctgggac atcctgtccc
     6841 ctcagttcat gtacggctcc aaggcctacg tgaagcaccc cgccgacatc cccgactact
     6901 tgaagctgtc cttccccgag ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg
     6961 gcgtggtgac cgtgacccag gactcctccc tgcaggacgg cgagttcatc tacaaggtga
     7021 agctgcgcgg caccaacttc ccctccgacg gccccgtaat gcagaagaag accatgggct
     7081 gggaggcctc ctccgagcgg atgtaccccg aggacggcgc cctgaagggc gagatcaagc
     7141 agaggctgaa gctgaaggac ggcggccact acgacgctga ggtcaagacc acctacaagg
     7201 ccaagaagcc cgtgcagctg cccggcgcct acaacgtcaa catcaagttg gacatcacct
     7261 cccacaacga ggactacacc atcgtggaac agtacgaacg cgccgagggc cgccactcca
     7321 ccggcggcat ggacgagctg tacaagtaag gcgcgccacc gattcctccc gataagtaaa
     7381 ttacagaaaa gcttcaggtt aaatgtaaga ggatcttaaa tattgatttg gtttttgtct
     7441 taatggtgcc cggcgtattt ataagaacca ttaaatttca ctagaccgtt tgtagtcagg
     7501 ggtgaacgct gcatgaccca cgctctcaaa gtgagttgtg ttgctttgcg ggaatccagc
     7561 agtcacatta atgctggaaa aagttcaggc aactagtgta ctttgtagcg gggtccggga
     7621 gaggctgggg tctagagtgg cgatgggacg aaagggtaaa gaaagttttc ggtgtgtgtg
     7681 tgtgtgtgtg tgtgtgtgtg tgtgtgtgtg tgtgtgtagg taggtaggta ggtagaaact
     7741 gcatcctctg cttgtgtaaa tccggttaca tgtgtgtaac agaggtactg agatgtgact
     7801 gagtctcaag gccaggcgat cgggattcag aagaggcatt tgcataactg agcaagagcc
     7861 tttgccccac ccttccacgc ggacccaaga caagcggaca gctctgggtg ggtcaccttg
     7921 aagccagggg cccgcccaca tccccgccca cacccacctt gagcgcttct cattgggcat
     7981 tccagcctac ccagctcgga gttagttact ccgtaagtgt ggccggaaca gagttcgtcc
     8041 atctaaaaag ggaggggacc ggcgaacaca tccggtagga gggccatcat ggccaagcct
     8101 ttgtctcaag aagaatccac cctcattgaa agagcaacgg ctacaatcaa cagcatcccc
     8161 atctctgaag actacagcgt cgccagcgca gctctctcta gcgacggccg catcttcact
     8221 ggtgtcaatg tatatcattt tactggggga ccttgtgcag aactcgtggt gctgggcact
     8281 gctgctgctg cggcagctgg caacctgact tgtatcgtcg cgatcggaaa tgagaacagg
     8341 ggcatcttga gcccctgcgg acggtgccga caggtgcttc tcgatctgca tcctgggatc
     8401 aaagccatag tgaaggacag tgatggacag ccgacggcag ttgggattcg tgaattgctg
     8461 ccctctggtt atgtgtggga gggctaacta gaaatcaacc tctggattac aaaatttgtg
     8521 aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt
     8581 taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata
     8641 aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg
     8701 tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc
     8761 tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct
     8821 gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt
     8881 cggggaagct gacgtccttt ccatggctgc tcgcctgtgt tgccacctgg attctgcgcg
     8941 ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc
     9001 tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct
     9061 ccctttgggc cgcctccccg cctggtcgac aggagctttg ttccttgggt tcttgggagc
     9121 agcaggaagc actatgggct gcacgtcaat gacgctgacg gtacaggcca gacaattatt
     9181 gtctgatata gtgcagcagc agaacaattt gctgagggct attgaggcgc aacagcatct
     9241 gttgcaactc acagtctggg gcatcaaaca gctccaggca agaatcctgg ctgtggaaag
     9301 atacctaaag gatcaacagc tcctggtacc tttaagacca atgacttaca aggcagctgt
     9361 agatcttagc cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg
     9421 aagacaagat atcctcgaga taacttcgta tagcatacat tatacgaagt tatcagtcga
     9481 gtttatttgt gaaatttgtg atgctattgc tttatttgta agcagctgct ttttgcctgt
     9541 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac
     9601 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg
     9661 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct
     9721 agcagccaaa cgcgtccgcg gtgtacccgg cgccacaggt gcggttgctg gcgcctatat
     9781 cgccgacatc accgatgggg aagatcgggc tcgccacttc gggctcatga gcgcttgttt
     9841 cggcgtgggt atggtggcag gccccgtggc cgggggactg ttgggcgcca tctccttgca
     9901 tgcaccattc cttgcggcgg cggtgctcaa cggcctcaac ctactactgg gctgcttcct
     9961 aatgcaggag tcgcataagg gagagcgtcg aatggtgcac tctcagtaca atctgctctg
    10021 atgccgcata gttaagccag ccccgacacc cgccaacacc cgctgacgcg ccctgacggg
    10081 cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt
    10141 gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc
    10201 tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc
    10261 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
    10321 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga
    10381 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt
    10441 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag
    10501 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag
    10561 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta
    10621 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg
    10681 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca
    10741 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag
    10801 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc
    10861 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg
    10921 tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc
    10981 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg
    11041 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg
    11101 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga
    11161 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac
    11221 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa
    11281 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
    11341 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
    11401 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
    11461 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
    11521 ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc
    11581 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
    11641 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
    11701 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
    11761 gaacgaccta caccgaactg agatacctac agcgtgagca ttgagaaagc gccacgcttc
    11821 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
    11881 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
    11941 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
    12001 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
    12061 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
    12121 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc
    12181 gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctgtggaa
    12241 tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
    12301 catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag
    12361 aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc
    12421 catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt
    12481 ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg
    12541 aggctttttt ggaggcctag gctagagatc ataatcagcc ataccacatt tgtagaggtt
    12601 ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa aatgaatgca
    12661 attgttgttg ttaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc
    12721 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc
    12781 atcaatgtat cttatcatgt ctggatc
//
