LOCUS       sequence-98334-e         749 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Luciferase reporter to measure miR-155 mediated differential 
            repression. Contains LMBRD2 3' UTR and mutated miR-155 sites.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 749)
  AUTHORS   Nam JW, Rissland OS, Koppstein D, Abreu-Goodger C, Jan CH, Agarwal 
            V, Yildirim MA, Rodriguez A, Bartel DP
  TITLE     Global analyses of the effect of different cellular contexts on 
            microRNA targeting.
  JOURNAL   Mol Cell. 2014 Mar 20;53(6):1031-43. doi: 
            10.1016/j.molcel.2014.02.013. Epub 2014 Mar 13.
  PUBMED    24631284
REFERENCE   2  (bases 1 to 749)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2014.02.013"; journalName: "Mol Cell"; date: 
            "2014-03-20- 20"; volume: "53"; issue: "6"; pages: "1031-43"
COMMENT     Sequence Label: pIS1 mLMBRD2
FEATURES             Location/Qualifiers
     source          1..749
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    609..730
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(620..639)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     674..693
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 aaaggtcttc atttttcgca agaagatgca cctgatgaaa tgggaaaata tatcaaatcg
       61 ttcgttgagc gagttctcaa aaatgaacaa taattctagg agctctatac cggtctcgat
      121 atcactacta gtgttctaga cttcaagtga tgcgtgtctg tgtatttagc atgtgtacta
      181 taatcaggtg atatagtatt ccttcagtct ttgtagtaac tggatttttt tatgcttctg
      241 gtattgcttt ataaaagatt ttcatttcag aaagctatgt tcacatttat catttaacat
      301 attgatttaa atgttgatta tgatattcaa ggataaatcc tagacgtaaa tgaaaaatag
      361 ttttttttta agtagtcaaa gagtccaaca aaatccttca gtatttaaag gtacctgctt
      421 atactttttg agaaaaaaac tggttattca aaggtaaaaa gtgaatccat ttactgttat
      481 tactgatact taaatcgtgg tttatagaaa acaaaactat attagaatgg aaatgtgtaa
      541 taacttgttg gttaaacatt cttactaaat agactgtttt taaccgcggc cgcttcgagc
      601 agacatgata agatacattg atgagtttgg acaaaccaca actagaatgc agtgaaaaaa
      661 atgctttatt tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa
      721 taaacaagtt aacaacaaca attgcattc
//
