LOCUS       sequence-98337-e         834 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Shuttle vector for us in AdEasy System. For expression of transgenes
            under a CMV promoter when a GFP tracer is desired..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 834)
  AUTHORS   He TC, Zhou S, da Costa LT, Yu J, Kinzler KW, Vogelstein B
  TITLE     A simplified system for generating recombinant adenoviruses.
  JOURNAL   Proc Natl Acad Sci U S A. 1998 Mar 3. 95(5):2509-14.
  PUBMED    9482916
REFERENCE   2  (bases 1 to 834)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 1998 Mar 3. 95(5):2509-14."
COMMENT     Sequence Label: pAdTrack-CMV
FEATURES             Location/Qualifiers
     source          1..834
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    165..286
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(202..221)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     256..275
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     misc_signal     429..579
                     /label=Ad5 Psi
                     /note="packaging signal for adenovirus serotype 5"
     repeat_region   667..769
                     /label=ITR
                     /note="inverted terminal repeat of human adenovirus 
                     serotype 5"
ORIGIN
        1 ctctcggcat ggacgagctg tacaagtccg gactcagatc caccggatct agataactga
       61 tcataatcag ccataccaca tttgtagagg ttttacttgc tttaaaaaac ctcccacacc
      121 tccccctgaa cctgaaacat aaaatgaatg caattgttgt tgttaacttg tttattgcag
      181 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
      241 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttaacgc gatcaagcta
      301 gcttgctaga ctcgactgac tataataata aaacgccaac tttgacccgg aacgcggaaa
      361 acacctgaga aaaacacctg ggcgagtctc cacgtaaacg gtcaaagtcc ccgcggccct
      421 agacaaatat tacgcgctat gagtaacaca aaattattca gatttcactt cctcttattc
      481 agttttcccg cgaaaatggc caaatcttac tcggttacgc ccaaatttac tacaacatcc
      541 gcctaaaacc gcgcgaaaat tgtcacttcc tgtgtacacc ggcgcacacc aaaaacgtca
      601 cttttgccac atccgtcgct tacatgtgtt ccgccacact tgcaacatca cacttccgcc
      661 acactactac gtcacccgcc ccgttcccac gccccgcgcc acgtcacaaa ctccaccccc
      721 tcattatcat attggcttca atccaaaata aggtatatta ttgatgatgt taattaagaa
      781 ttaattcgat cctgaatggc gaatggacgc gccctgtagc ggcgcattaa gcgc
//
