LOCUS       sequence-98338-e         776 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Luciferase reporter to measure miR-155 mediated differential 
            repression. Contains INSIG2 3' UTR and wild-type miR-155 sites.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 776)
  AUTHORS   Nam JW, Rissland OS, Koppstein D, Abreu-Goodger C, Jan CH, Agarwal 
            V, Yildirim MA, Rodriguez A, Bartel DP
  TITLE     Global analyses of the effect of different cellular contexts on 
            microRNA targeting.
  JOURNAL   Mol Cell. 2014 Mar 20;53(6):1031-43. doi: 
            10.1016/j.molcel.2014.02.013. Epub 2014 Mar 13.
  PUBMED    24631284
REFERENCE   2  (bases 1 to 776)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2014.02.013"; journalName: "Mol Cell"; date: 
            "2014-03-20- 20"; volume: "53"; issue: "6"; pages: "1031-43"
COMMENT     Sequence Label: pIS1 INSIG2
FEATURES             Location/Qualifiers
     source          1..776
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(685..704)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     739..758
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 aagtaaaagg tcttcatttt tcgcaagaag atgcacctga tgaaatggga aaatatatca
       61 aatcgttcgt tgagcgagtt ctcaaaaatg aacaataatt ctaggagctc tataccggtc
      121 tcgatatcac tactagtgtt ctagagtatt ttctctttag cttactgttg tgccttttta
      181 tttttctaat cacagcagta tgagttatga gtgccctaat ttgtggttag tttctaattt
      241 aatgttgttt catagagttt ggagtgtttt gatacagggt gaaaatgaac ttctggtttc
      301 aaacctgcgt tactggagac agcccaaaga gtaattttct gttttgacag gttttactgg
      361 aagtatatgt gatgagcaga agaggttatc agcattaaat tgttttggtt ctaaatttgg
      421 aacagtatat ataattaaaa gtaaggaaca ttagaggatt taattagaat aaatacatgt
      481 tttggaaata cagtgacctc ttgcagtgtc acaaaagtgc aaagtgatat tagctgtcat
      541 ctgcaataca gaatctcatt gcttttgcac atggagcata taggaaactc caaacagatc
      601 acaatgaggt ttctaaatct gttgggttct gtcttctatt gggttctgtg gcggccgctt
      661 cgagcagaca tgataagata cattgatgag tttggacaaa ccacaactag aatgcagtga
      721 aaaaaatgct ttatttgtga aatttgtgat gctattgctt tatttgtaac cattat
//
