LOCUS       sequence-98346-e        1055 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1055)
  TITLE     Lowe Lab Plasmids
REFERENCE   2  (bases 1 to 1055)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: MSCV Luciferase PGK-hygro
FEATURES             Location/Qualifiers
     source          1..1055
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     7..23
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     promoter        58..254
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      105..240
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     regulatory      284..293
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(356..374)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
ORIGIN
        1 tcctcccttt atccagccct cactccttct ctaggcgccg gaattagatc tgcgatctgc
       61 atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc
      121 cgcccagttc cgcccattct ccgccccatc gctgactaat tttttttatt tatgcagagg
      181 ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc
      241 taggcttttg caaaaagctt ggcattccgg tactgttggt aaagccacca tggaagacgc
      301 caaaaacata aagaaaggcc cggcgccatt ctatccgctg gaagatggaa ccgctggaga
      361 gcaactgcat aaggctatga agagatacgc cctggttcct ggaacaattg cttttacaga
      421 tgcacatatc gaggtggaca tcacttacgc tgagtacttc gaaatgtccg ttcggttggc
      481 agaagctatg aaacgatatg ggctgaatac aaatcacaga atcgtcgtat gcagtgaaaa
      541 ctctcttcaa ttctttatgc cggtgttggg cgcgttattt atcggagttg cagttgcgcc
      601 cgcgaacgac atttataatg aacgtgaatt gctcaacagt atgggcattt cgcagcctac
      661 cgtggtgttc gtttccaaaa aggggttgca aaaaattttg aacgtgcaaa aaaagctccc
      721 aatcatccaa aaaattatta tcatggattc taaaacggat taccagggat ttcagtcgat
      781 gtacacgttc gtcacatctc atctacctcc cggttttaat gaatacgatt ttgtgccaga
      841 gtccttcgat agggacaaga caattgcact gatcatgaac tcctctggat ctactggtct
      901 gcctaaaggt gtcgctctgc ctcatagaac tgcctgcgtg agattctcgc atgccagaga
      961 tcctattttt ggcaatcaaa tcattccgga tactgcgatt ttaagtgttg ttccattcca
     1021 tcacggtttt ggaatgttta ctacactcgg atatt
//
