LOCUS       sequence-98349-e         701 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Cre recombinase and Renilla luciferase from the EFS 
            promoter and one U6-driven sgRNA. AAV backbone with SapI spacer for 
            sgRNA cloning..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 701)
  AUTHORS   Platt RJ, Chen S, Zhou Y, Yim MJ, Swiech L, Kempton HR, Dahlman JE, 
            Parnas O, Eisenhaure TM, Jovanovic M, Graham DB, Jhunjhunwala S, 
            Heidenreich M, Xavier RJ, Langer R, Anderson DG, Hacohen N, Regev A,
            Feng G, Sharp PA, Zhang F
  TITLE     CRISPR-Cas9 Knockin Mice for Genome Editing and Cancer Modeling.
  JOURNAL   Cell. 2014 Sep 24. pii: S0092-8674(14)01163-5. doi: 
            10.1016/j.cell.2014.09.014.
  PUBMED    25263330
REFERENCE   2  (bases 1 to 701)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2014 
            Sep 24. pii: S0092-8674(14)01163-5. doi: 
            10.1016/j.cell.2014.09.014."
COMMENT     Sequence Label: 
            AAV:ITR-U6-sgRNA(backbone)-pEFS-Rluc-2A-Cre-WPRE-hGHpA-ITR
FEATURES             Location/Qualifiers
     source          1..701
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 tttttcccga gggtggggga gaaccgtata taagtgcagt agtcgccgtg aacgttcttt
       61 ttcgcaacgg gtttgccgcc agaacacagg cgtacggcca ccatgacttc gaaagtttat
      121 gatccagaac aaaggaaacg gatgataact ggtccgcagt ggtgggccag atgtaaacaa
      181 atgaatgttc ttgattcatt tattaattat tatgattcag aaaaacatgc agaaaatgct
      241 gttatttttt tacatggtaa cgcggcctct tcttatttat ggcgacatgt tgtgccacat
      301 attgagccag tagcgcggtg tattatacca gaccttattg gtatgggcaa atcaggcaaa
      361 tctggtaatg gttcttatag gttacttgat cattacaaat atcttactgc atggtttgaa
      421 cttcttaatt taccaaagaa gatcattttt gtcggccatg attggggtgc ttgtttggca
      481 tttcattata gctatgagca tcaagataag atcaaagcaa tagttcacgc tgaaagtgta
      541 gtagatgtga ttgaatcatg ggatgaatgg cctgatattg aagaagatat tgcgttgatc
      601 aaatctgaag aaggagaaaa aatggttttg gagaataact tcttcgtgga aaccatgttg
      661 ccatcaaaaa tcatgagaaa gttagaacca gaagaatttg c
//
