LOCUS       sequence-98359-e         812 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Luciferase reporter to measure miR-155 mediated differential 
            repression. Contains IL6R 3' UTR and wild-type miR-155 sites.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 812)
  AUTHORS   Nam JW, Rissland OS, Koppstein D, Abreu-Goodger C, Jan CH, Agarwal 
            V, Yildirim MA, Rodriguez A, Bartel DP
  TITLE     Global analyses of the effect of different cellular contexts on 
            microRNA targeting.
  JOURNAL   Mol Cell. 2014 Mar 20;53(6):1031-43. doi: 
            10.1016/j.molcel.2014.02.013. Epub 2014 Mar 13.
  PUBMED    24631284
REFERENCE   2  (bases 1 to 812)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2014.02.013"; journalName: "Mol Cell"; date: 
            "2014-03-20- 20"; volume: "53"; issue: "6"; pages: "1031-43"
COMMENT     Sequence Label: pIS1 IL6R
FEATURES             Location/Qualifiers
     source          1..812
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    669..790
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(680..699)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     734..753
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 aagtaaaagg tcttcatttt tcgcaagaag atgcacctga tgaaatggga aaatatatca
       61 aatcgttcgt tgagcgagtt ctcaaaaatg aacaataatt ctaggagctc tataccggtc
      121 tcgatatcac tactagtgtt ctagagtgtc tcaatgcacg gggcatttct acctgtgttt
      181 ctgcagcacc cccactgcct tgagtcccca gcagtgctgt tatttgccta acacctgtag
      241 ccatctgcca cgcagccaga cgtgaaacgc tgagacagag accatttagg ttaaatacga
      301 cagcttatcc tgctgggtgg ggaaagtaaa aaatatgctg gttcaaggcc taaagtaaaa
      361 tgatcaataa tgtttgtagc attaatgaaa tattttcaag aaatgtgtcc aggggtagca
      421 ctggctatgt tgacgaggcc tttggtaact cagagagctc ttggccctga tggggacttg
      481 cccttacgct ttctttatca ggctctgagt tcacacggag cctctggcac ttccctgctg
      541 tcttgggaga aaggaaactg gttgccgcgg caggttgtgg aatctgttgc tggaaccagg
      601 ctggaagccc acctggtagt gaacagggcc cagtggggca ggctggcggc cgcttcgagc
      661 agacatgata agatacattg atgagtttgg acaaaccaca actagaatgc agtgaaaaaa
      721 atgctttatt tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa
      781 taaacaagtt aacaacaaca attgcattca tt
//
