LOCUS       sequence-9836-ex       12703 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12703)
  AUTHORS   Kita-Matsuo H, Barcova M, Prigozhina N, Salomonis N, Wei K, Jacot 
            JG, Nelson B, Spiering S, Haverslag R, Kim C, Talantova M, Bajpai R,
            Calzolari D, Terskikh A, McCulloch AD, Price JH, Conklin BR, Chen 
            HS, Mercola M
  TITLE     Lentiviral vectors and protocols for creation of stable hESC lines 
            for fluorescent tracking and drug resistance selection of 
            cardiomyocytes.
  JOURNAL   PLoS ONE. 2009  . 4(4):e5046.
  PUBMED    19352491
REFERENCE   2  (bases 1 to 12703)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS ONE. 
            2009  . 4(4):e5046."
COMMENT     Sequence Label: aMHC-Puro Rex-Blast
FEATURES             Location/Qualifiers
     source          1..12703
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        50..353
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        354..557
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     508..528
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             571..751
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    798..923
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    973..1089
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     primer_bind     1165..1181
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1165..1181
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     CDS             6642..7241
                     /codon_start=1
                     /gene="pac from Streptomyces"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVECPKDRATWCMTRKPGA"
     primer_bind     complement(6642..6661)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7138..7158
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             7985..8383
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    8389..8977
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8442..8462)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8860..8871)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     misc_feature    8987..9220
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     protein_bind    complement(9336..9369)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     LTR             9440..9620
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(9670..9689)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(9903..9922)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     10022..10044
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(10082..10100)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        10168..10272
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10273..10341,10342..11133)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10491..10510)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11304..11892
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11793..11812
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     12046..12063
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(12133..12153)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        12151..12448
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     promoter        12249..12459
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      12318..12453
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    12569..12703
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     12660..12679
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 gggccccaat tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac
       61 ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa
      121 tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt
      181 atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc
      241 ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat
      301 gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc
      361 ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga tttccaagtc
      421 tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa
      481 aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta cggtgggagg
      541 tctatataag cagagctggt ttagtgaacc gggtctctct ggttagacca gatctgagcc
      601 tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga
      661 gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga
      721 cccttttagt cagtgtggaa aatctctagc agtggcgccc gaacagggac ctgaaagcga
      781 aagggaaacc agaggagctc tctcgacgca ggactcggct tgctgaagcg cgcacggcaa
      841 gaggcgaggg gcggcgactg cagagtacgc caaaaatttt gactagcgga ggctagaagg
      901 agagagatgg gtgcgagagc gtcagtatta agcgggggaa aataagcccg ggatggcagt
      961 attcatccac aattttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat
     1021 agtagacata atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat
     1081 tcaaattttc gggtttatta cagggacagc ggcctatggg aatgaaagac cccacctgtc
     1141 gagctccacc gcggtggcgg ccgctctaga actagtggat cctgcaaggt cacacaaggg
     1201 tctccaccca ccaggtgccc tagtctcaat ttcagtttcc atgccttgtt ctcacaatgc
     1261 tggcctcccc agagctaatt tggactttgt ttttatttca aaagggcctg aatgaggagt
     1321 agatcttgtg ctacccagct ctaagggtgc ccgtgaagcc ctcagacctg gagcctttgc
     1381 aacagccctt taggtggaag cagaataaag caattttcct taaagccaaa atcctgcctc
     1441 tagactcttc ttctctgacc tcggtccctg ggctctaggg tggggaggtg gggcttggaa
     1501 gaagaaggtg gggaagtggc aaaagccgat ccctagggcc ctgtgaagtt cggagccttc
     1561 cctgtacagc actggctcat agatcctcct ccagccaaac atagcaagaa gtgatacctc
     1621 ctttgtgact tccccaggcc cagtacctgt caggttgaaa caggatttag agaagcctct
     1681 gaactcacct gaactctgaa gctcatccac caagcaagca cctaggtgcc actgctagtt
     1741 agtatcctac gctgataata tgcagagctg ggccacagaa gtcctggggt gtaggaactg
     1801 accagtgact tttcagtcgg caaaggtatg accccctcag cagatgtagt aatgtcccct
     1861 tagatcccat cccaggcagg tctctaagag gacatgggat gagagatgta gtcatgtggc
     1921 attccaaaca cagctatcca cagtgtccct tgccccttcc acttagccag gaggacagta
     1981 accttagcct atctttcttc ctccccatcc tcccaggaca caccccctgg tctgcagtat
     2041 tcatttcttc cttcacgtcc cctctgtgac ttccatttgc aaggcttttg acctctgcag
     2101 ctgctggaag atagagtttg gccctaggtg tggcaagcca tctcaagaga aagcagacaa
     2161 cagggggacc agattttgga aggatcagga actaaatcac tggcgggcct gggggtagaa
     2221 aaaagagtga gtgagtccgc tccagctaag ccaagctagt ccccgagata ctctgccaca
     2281 gctgggctgc tcggggtagc tttaggaatg tgggtctgaa agacaatggg attggaagac
     2341 atctctttga gtctcccctc aaccccacct acagacacac tcgtgtgtgg ccagactcct
     2401 gttcaacagc cctctgtgtt ctgaccactg agctaggcaa ccagagcatg ggccctgtgc
     2461 tgaggatgaa gagttggtta ccaatagcaa aaacagcagg ggagggagaa cagagaacga
     2521 aataaggaag gaagaaggaa aggccagtca atcagatgca gtcagaagag atgggaagcc
     2581 aacacacagc ttgagcagag gaaacagaaa agggagagat tctgggcata aggaggccac
     2641 agaaagaaga gcccaggccc cccaagtctc ctctttatac cctcatcccg tctcccaatt
     2701 aagcccactc ttcttcctag atcagacctg agctgcagcg aagagacccg tagggaggat
     2761 cacactggat gaaggagatg tgtggagaag tccagggcaa cctaagagcc agagcctaaa
     2821 agagcaagag ataaaggtgc ttcaaaggtg gccaggctgt gcacacagag ggtcgaggac
     2881 tggtggtaga gcctcaagat aaggatgatg ctcagaatgg gcgggggggg ggattctggg
     2941 ggggggagag agaaggtgag aaggagcctg gaacagagaa tctggaagcg ctggaaacga
     3001 taccataaag ggaagaaccc aggctacctt tagatgtaaa tcatgaaaga cagggagaag
     3061 ggaagctgga gagagtagaa ggaccccggg gcaagacatg gaagcaagga caagccaggt
     3121 tgagcgctcc gtgaaatcag cctgctgaag gcagagccct ggtatgagca ccagaacagc
     3181 agaggctagg gttaatgtcg agacagggaa cagaaggtag acacaggaac agacagagac
     3241 gggggagcca ggtaacaaag gaatggtcct tctcacctgt ggccagagcg tccatctgtg
     3301 tccacatact ctagaatgtt catcagactg cagggctggc ttgggaggca gctggaaaga
     3361 gtatgtgaga gccaggggag acaagggggc ctaggaaagg aagaagaggg caaaccaggc
     3421 cacacaagag ggcagagccc agaactgagt taactccttc cttgttgcat cttccatagg
     3481 aggcagtggg aactctgtga ccaccatccc ccatgagccc ccactaccca taccaagttt
     3541 ggcctgagtg gcattctagg ttccctgagg acagagcctg gcctttgtct cttggacctg
     3601 acccaagctg acccaatgtt ctcagtacct tatcatgccc tcaagagctt gagaaccagg
     3661 cagtgacata ttaggccatg ggctaaccct ggagcttgca cacaggagcc tcaagtgacc
     3721 tccagggaca cagctgcaga caggtggcct ttatccccaa agagcaacca tttggcatag
     3781 gtggctgcaa atgggaatgc aaggttgaat caggtccctt caagaatact gcatgcaaga
     3841 cctaagaccc ctggagagag gggtatgctc ctgcccccac ccaccataag gggagtgaac
     3901 tatcctaggg ggctggcgac cttggggaga caccacatta ctgagagtgc tgagcccaga
     3961 aaaactgacc gccctgtgtc ctgcccacct ccacactcta gagctatatt gagaggtgac
     4021 agtagatagg gtgggagctg gtagcaggga gagtgttcct gggtgtgagg gtgtagggga
     4081 aagccagagc aggggagtct ggctttgtct cctgaacaca atgtctactt agttataaca
     4141 ggcatgacct gctaaagacc caacatctac gacctctgaa aagacagcag ccctggagga
     4201 caggggttgt ctctgagcct tgggtgcttg atggtgccac aaaggagggc atgagtgtga
     4261 gtataaggcc ccaggagcgt tagagaaggg cacttgggaa ggggtcagtc tgcagagccc
     4321 ctatccatgg aatctggagc ctggggccaa ctggtgtaaa tctctgggcc tgccaggcat
     4381 tcaaagcagc acctgcatcc tctggcagcc tggggaggcg gaagggagca accccccact
     4441 tatacccttt ctccctcagc cccaggatta acacctctgg ccttccccct tcccacctcc
     4501 catcaggagt ggagggttgc agagggaggg taaaaaccta catgtccaaa catcatggtg
     4561 cacgatatat ggatcagtat gtgtagaggc aagaaaggaa atctgcaggc ttaactgggt
     4621 taatgtgtaa agtctgtgtg catgtgtgtg tgtctgactg aaaacgggca tggctgtgca
     4681 gctgttcagt tctgtgcgtg aggttaccag actgcaggtt tgtgtgtaaa ttgcccaagg
     4741 caaagtgggt gaatcccttc catggtttaa agagattgga tgatggcctg catctcaagg
     4801 accatggaaa atagaatgga cactctatat gtgtctctaa gctaaggtag caaggtcttt
     4861 ggaggacacc tgtctagaga tgtgggcaac agagactaca gacagtatct gtacagagta
     4921 aggagagaga ggagggggtg tagaattctc ttactatcaa agggaaactg agtcgtgcac
     4981 ctgcaaagtg gatgctctcc ctagacatca tgactttgtc tctggggagc cagcactgtg
     5041 gaacttcagg tctgagagag taggaggctc ccctcagcct gaagctatgc agatagccag
     5101 ggttgaaagg gggaagggag agcctgggat gggagcttgt gtgttggagg caggggacag
     5161 atattaagcc tggaagagaa ggtgaccctt acccagttgt tcaactcacc cttcagatta
     5221 aaaataactg aggtaagggc ctgggtaggg gaggtggtgt gagacgctcc tgtctctcct
     5281 ctatctgccc atcggccctt tggggaggag gaatgtgccc aaggactaaa aaaaggccat
     5341 ggagccagag gggcgagggc aacagacctt tcatgggcaa accttggggc cctgctgtcc
     5401 tcctgtcacc tccagagcca agggatcaaa ggaggaggag ccaggacagg agggaagtgg
     5461 gagggagggt cccagcagag gactccaaat ttaggcagca ggcatatggg atgggatata
     5521 aaggggctgg agcactgaga gctgtcagag atttctccaa cccaggtaag agggagtttc
     5581 gggtgggggc tcttcaccca caccagacct ctccccacct agaaggaaac tgcctttcct
     5641 ggaagtgggg ttcaggccgg tcagagatct gacagggtgg ccttccacca gcctgggaag
     5701 ttctcagtgg caggaggttt ccacaagaaa cactggatgc cccttccctt acgctgtctt
     5761 ctccatcttc ctcctgggga tgctcctccc cgtcttggtt tatcttggct cttcgtcttc
     5821 agcaagattt gccctgtgct gtccactcca tctttctcta ctgtctccgt gccttgcctt
     5881 gccttcttgc gtgtccttcc tttccaccca tttctcactt caccttttct ccccttctca
     5941 tttgtattca tccttccttc cttccttcct tccttccttc cttccttcct tccttccttt
     6001 ctcccttcct tccttccttc cttccttcct tccttccttc cttcctgtgt cagagtgctg
     6061 agaatcacac ctggggttcc cacccttatg taaacaatct tccagtgagc cacagcttca
     6121 gtgctgctgg gtgctctctt accttcctca ccccctggct tgtcctgttc catcctggtc
     6181 aggatctcta gattggtctc ccagcctctg ctactcctct tcctgcctgt tcctctctct
     6241 gtccagctgc gccactgtgg tgcctcgttc cagctgtggt ccacattctt caggattctc
     6301 tgaaaagtta accaggtgag aatgtttccc ctgtagacag cagatcacga ttctcccgga
     6361 agtcaggctt ccagccctct ctttctctgc ccagctgccc ggcactctta gcaaacctca
     6421 ggcaccctta ccccacatag acctctgaca gagaagcagg cactttacat ggagtcctgg
     6481 tgggagagcc ataggctacg gtgtaaaaga ggcagggaag tggtggtgta ggaaagtcag
     6541 gacttcacat agaagcctag cccacaccag aaatgacaga cagatccctc ctatctcccc
     6601 cataagagtt tgagtcgact gatcaaaaac cggtcgccac catgaccgag tacaagccca
     6661 cggtgcgcct cgccacccgc gacgacgtcc cccgggccgt acgcaccctc gccgccgcgt
     6721 tcgccgacta ccccgccacg cgccacaccg tcgacccgga ccgccacatc gagcgggtca
     6781 ccgagctgca agaactcttc ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg
     6841 cggacgacgg cgccgcggtg gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg
     6901 tgttcgccga gatcggcccg cgcatggccg agttgagcgg ttcccggctg gccgcgcagc
     6961 aacagatgga aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca
     7021 ccgtcggcgt ctcgcccgac caccagggca agggtctggg cagcgccgtc gtgctccccg
     7081 gagtggaggc ggccgagcgc gccggggtgc ccgccttcct ggagacctcc gcgccccgca
     7141 acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag tgcccgaagg
     7201 accgcgcgac ctggtgcatg acccgcaagc ccggtgcctg ataagggcgc gccaccgatt
     7261 cctcccgata agtaaattac agaaaagctt caggttaaat gtaagaggat cttaaatatt
     7321 gatttggttt ttgtcttaat ggtgcccggc gtatttataa gaaccattaa atttcactag
     7381 accgtttgta gtcaggggtg aacgctgcat gacccacgct ctcaaagtga gttgtgttgc
     7441 tttgcgggaa tccagcagtc acattaatgc tggaaaaagt tcaggcaact agtgtacttt
     7501 gtagcggggt ccgggagagg ctggggtcta gagtggcgat gggacgaaag ggtaaagaaa
     7561 gttttcggtg tgtgtgtgtg tgtgtgtgtg tgtgtgtgtg tgtgtgtgtg tgtaggtagg
     7621 taggtaggta gaaactgcat cctctgcttg tgtaaatccg gttacatgtg tgtaacagag
     7681 gtactgagat gtgactgagt ctcaaggcca ggcgatcggg attcagaaga ggcatttgca
     7741 taactgagca agagcctttg ccccaccctt ccacgcggac ccaagacaag cggacagctc
     7801 tgggtgggtc accttgaagc caggggcccg cccacatccc cgcccacacc caccttgagc
     7861 gcttctcatt gggcattcca gcctacccag ctcggagtta gttactccgt aagtgtggcc
     7921 ggaacagagt tcgtccatct aaaaagggag gggaccggcg aacacatccg gtaggagggc
     7981 catcatggcc aagcctttgt ctcaagaaga atccaccctc attgaaagag caacggctac
     8041 aatcaacagc atccccatct ctgaagacta cagcgtcgcc agcgcagctc tctctagcga
     8101 cggccgcatc ttcactggtg tcaatgtata tcattttact gggggacctt gtgcagaact
     8161 cgtggtgctg ggcactgctg ctgctgcggc agctggcaac ctgacttgta tcgtcgcgat
     8221 cggaaatgag aacaggggca tcttgagccc ctgcggacgg tgccgacagg tgcttctcga
     8281 tctgcatcct gggatcaaag ccatagtgaa ggacagtgat ggacagccga cggcagttgg
     8341 gattcgtgaa ttgctgccct ctggttatgt gtgggagggc taactagaaa tcaacctctg
     8401 gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta
     8461 tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt
     8521 ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc
     8581 aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt
     8641 gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg
     8701 gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac
     8761 aattccgtgg tgttgtcggg gaagctgacg tcctttccat ggctgctcgc ctgtgttgcc
     8821 acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac
     8881 cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct
     8941 cagacgagtc ggatctccct ttgggccgcc tccccgcctg gtcgacagga gctttgttcc
     9001 ttgggttctt gggagcagca ggaagcacta tgggctgcac gtcaatgacg ctgacggtac
     9061 aggccagaca attattgtct gatatagtgc agcagcagaa caatttgctg agggctattg
     9121 aggcgcaaca gcatctgttg caactcacag tctggggcat caaacagctc caggcaagaa
     9181 tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggtaccttta agaccaatga
     9241 cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc
     9301 taattcactc ccaacgaaga caagatatcc tcgagataac ttcgtatagc atacattata
     9361 cgaagttatc agtcgagttt atttgtgaaa tttgtgatgc tattgcttta tttgtaagca
     9421 gctgcttttt gcctgtactg ggtctctctg gttagaccag atctgagcct gggagctctc
     9481 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt
     9541 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc
     9601 agtgtggaaa atctctagca gccaaacgcg tccgcggtgt acccggcgcc acaggtgcgg
     9661 ttgctggcgc ctatatcgcc gacatcaccg atggggaaga tcgggctcgc cacttcgggc
     9721 tcatgagcgc ttgtttcggc gtgggtatgg tggcaggccc cgtggccggg ggactgttgg
     9781 gcgccatctc cttgcatgca ccattccttg cggcggcggt gctcaacggc ctcaacctac
     9841 tactgggctg cttcctaatg caggagtcgc ataagggaga gcgtcgaatg gtgcactctc
     9901 agtacaatct gctctgatgc cgcatagtta agccagcccc gacacccgcc aacacccgct
     9961 gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc
    10021 tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag
    10081 ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg
    10141 tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
    10201 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
    10261 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca
    10321 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat
    10381 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag
    10441 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc
    10501 gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct
    10561 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca
    10621 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt
    10681 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat
    10741 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt
    10801 gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta
    10861 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga
    10921 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt
    10981 gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc
    11041 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct
    11101 gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata
    11161 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
    11221 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
    11281 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
    11341 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
    11401 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg
    11461 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
    11521 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
    11581 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
    11641 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagcattga
    11701 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
    11761 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
    11821 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
    11881 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
    11941 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
    12001 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc
    12061 gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat
    12121 taatgcagct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag
    12181 gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag
    12241 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc
    12301 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc
    12361 atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat
    12421 tccagaagta gtgaggaggc ttttttggag gcctaggcta gagatcataa tcagccatac
    12481 cacatttgta gaggttttac ttgctttaaa aaacctccca cacctccccc tgaacctgaa
    12541 acataaaatg aatgcaattg ttgttgttaa cttgtttatt gcagcttata atggttacaa
    12601 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
    12661 tggtttgtcc aaactcatca atgtatctta tcatgtctgg atc
//
