LOCUS       sequence-98374-e         680 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 680)
  TITLE     SGC Toronto Q1-Q3 2013
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 680)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: 3HX4
FEATURES             Location/Qualifiers
     source          1..680
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     42..61
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        42..60
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    61..85
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             100..122
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             132..149
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             162..182
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa ttaatacgac tcactatagg
       61 ggaattgtga gcggataaca attcccctct agaaataatt ttgtttaact ttaagaagga
      121 gatataccat gcatcatcat catcatcaca gcagcggcag agaaaacttg tatttccagg
      181 gcatggggca gcaggaaagc actcttgggg gtgcggccgg ggagcctcgc tcgcgcggtc
      241 atgcggcggg gaccagcggt ggaccaggag accatctcca cgcgacgccc gggatgttcg
      301 ttcagcattc gactgcgatc ttctccgacc ggtacaaggg acagcgggtg ttggggaagg
      361 ggtcttttgg tgaggtgatt ctgtgcaaag acaagatcac cggtcaggag tgtgcggtga
      421 aggtcattag caagcgccaa gtgaagcaga agacggacaa ggagtctctg cttcgcgagg
      481 tgcagttgct gaagcagctg gaccacccca acatcatgaa gctgtatgaa ttcttcgagg
      541 acaaaggcta cttctacctc gtcggcgaag tgtacacggg aggcgagttg ttcgacgaga
      601 tcatttcccg caagcgcttc agcgaagtcg atgcggcgcg gatcatccgc caagtcctca
      661 gcggcatcac gtacatgcac
//
