LOCUS       sequence-98375-e         305 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 305)
  TITLE     SGC Toronto Q1-Q3 2013
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 305)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: 3C5I
FEATURES             Location/Qualifiers
     source          1..305
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    64..88
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             105..127
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             137..154
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             167..187
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 atgcgtccgg cgctagagga tcgagatctc gatcccgcgc aaataatacg actcacatat
       61 aggggaattg tgagcggata acaattcccc gtcgtagaaa taattttgtt taactttaag
      121 aaggagatat accatgcatc atcatcatca tcacagcagc ggcagagaaa acttgtattt
      181 ccagggcccc ttgtgtgccc aagaattctc ggacctaacg gatcctttat atattaaaaa
      241 aatatgcctg gaaaaagtac cagagtggaa tcattttacc gaagataatt taagggttaa
      301 gcaga
//
