LOCUS       sequence-98378-e         702 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Dre-responsive fluorescent reporter plasmid containing a 
            STOP-cassette flanked by Dre-recognition sites (rox) followed by 
            ZsGreen.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 702)
  AUTHORS   Hermann M, Stillhard P, Wildner H, Seruggia D, Kapp V, 
            Sanchez-Iranzo H, Mercader N, Montoliu L, Zeilhofer HU, Pelczar P
  TITLE     Binary recombinase systems for high-resolution conditional 
            mutagenesis.
  JOURNAL   Nucleic Acids Res. 2014 Jan 9.
  PUBMED    24413561
REFERENCE   2  (bases 1 to 702)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2014 Jan 9."
COMMENT     Sequence Label: pCAG-roxSTOProx-ZsGreen
FEATURES             Location/Qualifiers
     source          1..702
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     43..62
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        43..61
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    78..109
                     /label=rox
                     /bound_moiety="Dre recombinase"
     polyA_signal    276..410
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    530..664
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 catgttcatg ccttcttctt tttcctacag atccttaatt aataatacga ctcactatag
       61 ggggtcgacc cgccacctaa ctttaaataa ttggcattat ttaaagttat cgcgatgaat
      121 aaatgaaagc ttgcagatct gcgactctag aggatctgcg actctagagg atcataatca
      181 gccataccac atttgtagag gttttacttg ctttaaaaaa cctcccacac ctccccctga
      241 acctgaaaca taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg
      301 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
      361 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggatc tgcgactcta
      421 gaggatcata atcagccata ccacatttgt agaggtttta cttgctttaa aaaacctccc
      481 acacctcccc ctgaacctga aacataaaat gaatgcaatt gttgttgtta acttgtttat
      541 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
      601 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
      661 gatctgcgac tctagaggat cataatcagc cataccacat tt
//
