LOCUS       sequence-98386-e         903 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mitochondria-targeted genetically encoded fluorescent indicator for 
            measuring NAD/NADH ratio..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 903)
  AUTHORS   Bilan DS, Matlashov ME, Gorokhovatsky AY, Schultz C, Enikolopov G, 
            Belousov VV
  TITLE     Genetically encoded fluorescent indicator for imaging NAD/NADH ratio
            changes in different cellular compartments.
  JOURNAL   Biochim Biophys Acta. 2013 Nov 25. pii: S0304-4165(13)00519-9. doi: 
            10.1016/j.bbagen.2013.11.018.
  PUBMED    24286672
REFERENCE   2  (bases 1 to 903)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biochim 
            Biophys Acta. 2013 Nov 25. pii: S0304-4165(13)00519-9. doi: 
            10.1016/j.bbagen.2013.11.018."
COMMENT     Sequence Label: pC1-mitoRexYFP
FEATURES             Location/Qualifiers
     source          1..903
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             22..96
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
     CDS             118..189
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="SVLTPLLLRGLTGSARRLPVPRAK"
     primer_bind     687..708
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(817..838)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 tgaaccgtca gatccgctag catgtccgtc ctgacgccgc tgctgctgcg gggcttgaca
       61 ggctcggccc ggcggctccc agtgccgcgc gccaagatcc attcgttggg ggatctgtcc
      121 gtcctgacgc cgctgctgct gcggggcttg acaggctcgg cccggcggct cccagtgccg
      181 cgcgccaaga tccattcgtt gggggatcca ccggtcgcca ccatgaaggt ccccgaagcg
      241 gccatcagcc gcctgatcac ctacctccgc atcctggagg agctggaggc ccagggggtt
      301 caccggacga gctccgagca gcttggggag ctcgcccagg tcacggcctt ccaggtgcgg
      361 aaggaccttt cctacttcgg ctcctacggg acgcgcgggg tggggtacac cgtccccgtc
      421 ctgaagcggg agctccgcca catcctgggg ctcaaccgct ccgccggcta caacagcgac
      481 aacgtctata tcatggccga caagcagaag aacggcatca aggccaactt caagatccgc
      541 cacaacgtcg aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc
      601 ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcttccagtc cgtcctgagc
      661 aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg
      721 atcactcccg gcatggacga gctgaacaac gtggatggcg gtagcggtgg caccggcagc
      781 aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta
      841 aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg
      901 acc
//
