LOCUS       sequence-9841-ex        4688 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4688)
  AUTHORS   Stoick-Cooper CL, Weidinger G, Riehle KJ, Hubbert C, Major MB, 
            Fausto N, Moon RT
  TITLE     Distinct Wnt signaling pathways have opposing roles in appendage 
            regeneration.
  JOURNAL   Development. 2007 Feb . 134(3):479-89.
  PUBMED    17185322
REFERENCE   2  (bases 1 to 4688)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Development. 2007 Feb . 134(3):479-89."
COMMENT     Sequence Label: zWnt5b
FEATURES             Location/Qualifiers
     source          1..4688
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(33..461)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(143..164)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     355..374
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     588..610
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     602..619
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     603..619
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     626..645
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     677..693
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     677..693
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(2457..2473)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2458..2474)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     promoter        complement(2503..2521)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2542..2558)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2542..2558)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2555..2577)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2566..2582
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2590..2596,2597..2614,2615..2620))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2635..2656
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2773..2790)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2944..3532)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3024..3043)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3703..4494,4495..4563))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4326..4345
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4564..4668)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cacctaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag
       61 ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac
      121 cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga
      181 ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc
      241 accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg
      301 gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa
      361 gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac
      421 caccacaccc gccgcgctta atgcgccgct acagggcgcg tcccattcgc cattcaggct
      481 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa
      541 agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg
      601 ttgtaaaacg acggccagtg aattgtaata cgactcacta tagggcgaat tggagctcca
      661 ccgcggtggc ggccgctcta gaactagtgg atcccccggg ctgcaggaat tccctaacaa
      721 gtgtgcaaag ccatctttcc ctgaagtctt tcctattgac ggacaagctg ttcaaccaat
      781 aaagcacagg tttttgctcg ggatacatct ttcttccatt tcgacaatca acttttatcc
      841 aacttccaag aacttccatc tgaggaggcg ctttgcattt caatttcttg gaggaggaag
      901 gatggatgtg agaatgaacc aaggacacct acttctggca gtgaccctca tcgtctgcaa
      961 ctcacagctg ctggtggtcg ccaactcgtg gtggtcatta gccatgaacc ccatccagag
     1021 accggagatg tacatcattg gagcacagcc tctgtgcagc cagctgacgg gcctatctca
     1081 gggtcagagg aagctctgcc agctctatca ggaccacatg gtttatattg gagagggggc
     1141 gaagacgggc atcaaagagt gccagtatca gttcagacag aggcgatgga actgcagtac
     1201 agtggacaac acgtcagtgt tcggccgcgt catgcatata ggcagccgag aaacagcttt
     1261 tacgtacgcc gtcagcgcag cgggtgttgt gaatgctgtg agtcgagcgt gccgtgaggg
     1321 tgagctttcc acctgcggct gcagtcgagc ggctcgtccc agagacctgc ccagagactg
     1381 gctgtggggc ggctgcgggg acaacgtcaa ctatggctac cgcttcgccc gggagtttgt
     1441 ggacgctcgt gaacgtgaga agaactaccc acgcggatcg gtggaacacg cacgcacgct
     1501 tatgaatctg cagaacaatg aagccggaag aatggcggtg tataatctag cgaatgtggc
     1561 ctgcaagtgt catggcgtct caggctcgtg cagcttgaaa acctgctggc tccagctggc
     1621 cgacttccgg cgtgttggag aattcctgaa ggagaaatac gacagcgccg ccgccatgcg
     1681 cattaaccga cgtggaaaac tagagctggt caataatcga ttcaacccac cgacaggtga
     1741 agatctggtc tacatcgacc ccagcccgga ttactgcctg cgcaatgaaa ccactgggtc
     1801 tctgggcacc caaggccgcc tatgcaacaa gacctcggag ggtatggacg gctgcgagct
     1861 catgtgctgc ggccgcgggt acgaccagtt caagacctac aaacatgagc gctgccactg
     1921 caagttccac tggtgctgct atgtcaagtg caaacgctgc acgtcactcg tagaccagtt
     1981 tgtgtgcaag tagcagacgt gagaactggg ggacagacgc actgagcaat taagcaggag
     2041 aagaaacggg acccttacgg acccagaggg gcaacttaga gataattaaa tgtaaaaatg
     2101 atatattaaa tagcaacaaa ttaaaagtat ataaataagt gtacgtgtgc cgttgatagt
     2161 aatttaatga cctgaggatc cacgttggac gtcattgatg aaaaaggagg caccggtgtg
     2221 atgcattccg gctcaagctg tgtttctctt atatagagag gaaggaactc ttgacttgtg
     2281 ctacaagaaa ctctttagag actcgaggaa aaggcgcaga aataggatgg ggaacatcaa
     2341 gggcgcatca cccacccatt gccttccaat tcttaaacac acacacacac acacacactc
     2401 caccttgctg atgtcagagc tcttaagaac tttcggaatg cggatatcaa gcttatcgat
     2461 accgtcgacc tcgagggggg gcccggtacc cagcttttgt tccctttagt gagggttaat
     2521 ttcgagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac
     2581 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt
     2641 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
     2701 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
     2761 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     2821 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     2881 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     2941 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     3001 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     3061 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     3121 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     3181 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     3241 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     3301 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     3361 gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt
     3421 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     3481 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     3541 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     3601 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     3661 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
     3721 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
     3781 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
     3841 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
     3901 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
     3961 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
     4021 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
     4081 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
     4141 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
     4201 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
     4261 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
     4321 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
     4381 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
     4441 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
     4501 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
     4561 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
     4621 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
     4681 aaaagtgc
//
