LOCUS       sequence-98423-e         739 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  encodes multiple copies of an epitope tag FLAG.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 739)
  AUTHORS   Viswanathan S, Williams ME, Bloss EB, Stasevich TJ, Speer CM, Nern 
            A, Pfeiffer BD, Hooks BM, Li WP, English BP, Tian T, Henry GL, 
            Macklin JJ, Patel R, Gerfen CR, Zhuang X, Wang Y, Rubin GM, Looger 
            LL
  TITLE     High-performance probes for light and electron microscopy.
  JOURNAL   Nat Methods. 2015 Jun;12(6):568-76. doi: 10.1038/nmeth.3365. Epub 
            2015 Apr 27.
  PUBMED    25915120
REFERENCE   2  (bases 1 to 739)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.3365"; journalName: "Nat Methods"; date: "2015-06"; 
            volume: "12"; issue: "6"; pages: "568-76"
COMMENT     Sequence Label: pCAG_smFP FLAG
FEATURES             Location/Qualifiers
     source          1..739
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(36..59)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(63..86)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(90..113)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(324..347)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(354..377)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(390..413)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(420..443)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 ttagctattc atcggtacct cgagcaccgg gtttatttat catcgtcgtc tttgtagtca
       61 cccttgtcat catcatcttt gtagtcgccc ttgtcgtcgt catctttata atctccgccc
      121 ttgtaaagct catccatgcc gagggtgatt cctgcggcgg tcacgaactc cagcagcacc
      181 atgtggtcac gcttctcgtt cgggtccttc gacagcaccg actgggtgga caagtagtgg
      241 ttatcaggca gcaggactgg accgtcacca atgggcgtgt tctgttggta atgatctgcc
      301 agctgcacgc ttccgtcgcc tcccttatca tcatcatcct tgtaatcttg ctgcttatcg
      361 tcatcgtcct tgtaatcgcc ttgctgacct ttgtcatcat cgtccttata gtcttgttgt
      421 ttgtcatcgt catccttata gtcgcctccc tccacattgt gacgaatctt gaaattagcc
      481 ttgatgccgt tcttctgctt gtcggcagta atgtaaacat tgtggctgtt aaaattgtac
      541 tccagcttgt ggcccagaat atttccgtcc tccttgaaat cgatgccttt gagctcgata
      601 cgattaacca gggtgtctcc ttcgaacttg acctcggcgc gagtcttgta ggtgccgtca
      661 tccttaaaac tgatggtgcg ctcctgcacg tagccttcgg gcatggccga cttgaagaag
      721 tcatgctgct tcatgtgat
//
