LOCUS       sequence-98424-e         743 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  encodes multiple copies of an epitope tag FLAG ( not detected by 
            anti GFP ab).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 743)
  AUTHORS   Viswanathan S, Williams ME, Bloss EB, Stasevich TJ, Speer CM, Nern 
            A, Pfeiffer BD, Hooks BM, Li WP, English BP, Tian T, Henry GL, 
            Macklin JJ, Patel R, Gerfen CR, Zhuang X, Wang Y, Rubin GM, Looger 
            LL
  TITLE     High-performance probes for light and electron microscopy.
  JOURNAL   Nat Methods. 2015 Jun;12(6):568-76. doi: 10.1038/nmeth.3365. Epub 
            2015 Apr 27.
  PUBMED    25915120
REFERENCE   2  (bases 1 to 743)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.3365"; journalName: "Nat Methods"; date: "2015-06"; 
            volume: "12"; issue: "6"; pages: "568-76"
COMMENT     Sequence Label: pCAG_mRuby2_smFP FLAG
FEATURES             Location/Qualifiers
     source          1..743
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(38..61)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(65..88)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(92..115)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(308..331)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(338..361)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(374..397)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(404..427)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 gacttagcta ttatcggtac ctcgagcacc gggtctactt atcatcatca tccttgtaat
       61 cccctttgtc atcgtcgtct ttatagtcac ccttgtcatc atcgtctttg taatcgcctc
      121 caccgcctcc cagtcccgcg aacttggcca cggcatgttc tctttgcacg acgaacatct
      181 cattgtctga ttcttcgagc cgctccaggc ggtggtcgac ggcatgaatg cccggcatct
      241 tgatgtttcc cacggttttc tttgatctgt aggtggtcac gaacgaacag gacagatgtc
      301 ctcctccttt atcatcgtca tctttgtagt cctgctgctt gtcgtcgtcg tccttgtagt
      361 cgccctgctg gcccttgtca tcatcgtctt tgtaatcttg ttgcttatcg tcgtcatcct
      421 tgtaatctcc tccgtcgacc ttcagcgcca tgtgggtgta gcccctcagc cctccgtctg
      481 cggggtacat catttcagta ttaggttccc aacccttcgt ctttttctgc atcactggtc
      541 cgttggacgg gaaattcact ccgcggactt ggacgtggta cacgaggcac ccatcctcca
      601 gcgaagtatc ttgcatgaca gtcaccacac ctccatcttc ataccgggtc acccgttccc
      661 aagtgaatcc ttccgggaat gactgcttaa agaagtccgg gattcccttc gggtacttga
      721 tgaacgtgcg cgaccctcct ccg
//
