LOCUS       sequence-98426-e         741 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Recombinant protein expression of Park7.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 741)
  AUTHORS   Lakshminarasimhan M, Maldonado MT, Zhou W, Fink AL, Wilson MA
  TITLE     Structural impact of three Parkinsonism-associated missense 
            mutations on human DJ-1.
  JOURNAL   Biochemistry. 2008 Feb 5;47(5):1381-92. doi: 10.1021/bi701189c. Epub
            2008 Jan 9.
  PUBMED    18181649
REFERENCE   2  (bases 1 to 741)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/bi701189c";
            journalName: "Biochemistry"; date: "2008-02-5- 5"; volume: "47"; 
            issue: "5"; pages: "1381-92"
COMMENT     Sequence Label: DJ-1 pET15b
FEATURES             Location/Qualifiers
     source          1..741
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(592..609)
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             complement(619..636)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             655..677
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    692..716
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(716..735)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(717..735)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 agcagccgga tcctcgagct agtctttaag aacaagtgga gccttcactt gagccgccac
       61 ctccttgcca ttcagggctt caacaattgc aagcgcaaac tcgaagctgg tcccaggccc
      121 ccggcttgta agaatcaggc cgtctttttc cacacgattc tcagagtagg tgtaatgacc
      181 tccattcatc attttgtctt tagcaagagg gtgtgttgta actttacttc caaaacctat
      241 ttcatgagcc aacagagcag taggacctgc acagatggcg gctatcaggc ccttccggtt
      301 ttcctgctcc ttcagtatct ccttcacagc agcagactca gataaattct gtgcgcccag
      361 attacctcct ggtagaacca ccacatcata aggtccctct ttttttgcat cttcaaggct
      421 ggcatcagga caaatgacca catcacggct acactgtact gggtcttttc cagccaggcc
      481 tgcaacggtg accttaatcc cagctcgcct catgacatct acagggatga ccgtctccat
      541 ttcctctgct cctttagcca ggatgaccag agctcttttg gaagccatat ggctgccgcg
      601 cggcaccagg ccgctgctgt gatgatgatg atgatggctg ctgcccatgg tatatctcct
      661 tcttaaagtt aaacaaaatt atttctagag gggaattgtt atccgctcac aattccccta
      721 tagtgagtcg tattaatttc g
//
