LOCUS       sequence-98436-e         714 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Recombinant protein expression of Park7.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 714)
  AUTHORS   Lakshminarasimhan M, Maldonado MT, Zhou W, Fink AL, Wilson MA
  TITLE     Structural impact of three Parkinsonism-associated missense 
            mutations on human DJ-1.
  JOURNAL   Biochemistry. 2008 Feb 5;47(5):1381-92. doi: 10.1021/bi701189c. Epub
            2008 Jan 9.
  PUBMED    18181649
REFERENCE   2  (bases 1 to 714)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/bi701189c";
            journalName: "Biochemistry"; date: "2008-02-5- 5"; volume: "47"; 
            issue: "5"; pages: "1381-92"
COMMENT     Sequence Label: M26I DJ-1 pET15b
FEATURES             Location/Qualifiers
     source          1..714
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             42..59
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             69..86
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 ttttgtttac tttaagaagg agatatacca tgggcagcag ccatcatcat catcatcaca
       61 gcagcggcct ggtgccgcgc ggcagccata tggcttccaa aagagctctg gtcatcctgg
      121 ctaaaggagc agaggaaatg gagacggtca tccctgtaga tgtcatcagg cgagctggga
      181 ttaaggtcac cgttgcaggc ctggctggaa aagacccagt acagtgtagc cgtgatgtgg
      241 tcatttgtcc tgatgccagc cttgaagatg caaaaaaaga gggaccttat gatgtggtgg
      301 ttctaccagg aggtaatctg ggcgcacaga atttatctga gtctgctgct gtgaaggaga
      361 tactgaagga gcaggaaaac cggaagggcc tgatagccgc catctgtgca ggtcctactg
      421 ctctgttggc tcatgaaata ggttttggaa gtaaagttac aacacaccct cttgctaaag
      481 acaaaatgat gaatggaggt cattacacct actctgagaa tcgtgtggaa aaagacggcc
      541 tgattcttac aagccggggg cctgggacca gcttcgagtt tgcgcttgca attgttgaag
      601 ccctgaatgg caaggaggtg gcggctcaag tgaaggctcc acttgttctt aaagactagc
      661 tcgaggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gcca
//
