LOCUS       sequence-98438-e         646 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Recombinant protein expression of Park7.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 646)
  AUTHORS   Lakshminarasimhan M, Maldonado MT, Zhou W, Fink AL, Wilson MA
  TITLE     Structural impact of three Parkinsonism-associated missense 
            mutations on human DJ-1.
  JOURNAL   Biochemistry. 2008 Feb 5;47(5):1381-92. doi: 10.1021/bi701189c. Epub
            2008 Jan 9.
  PUBMED    18181649
REFERENCE   2  (bases 1 to 646)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/bi701189c";
            journalName: "Biochemistry"; date: "2008-02-5- 5"; volume: "47"; 
            issue: "5"; pages: "1381-92"
COMMENT     Sequence Label: A104T DJ-1 pET15b
FEATURES             Location/Qualifiers
     source          1..646
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             2..24
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             43..60
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             70..87
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 ttttgtttaa ctttaagaag gagatatacc atgggcagca gccatcatca tcatcatcac
       61 agcagcggcc tggtgccgcg cggcagccat atggcttcca aaagagctct ggtcatcctg
      121 gctaaaggag cagaggaaat ggagacggtc atccctgtag atgtcatgag gcgagctggg
      181 attaaggtca ccgttgcagg cctggctgga aaagacccag tacagtgtag ccgtgatgtg
      241 gtcatttgtc ctgatgccag ccttgaagat gcaaaaaaag agggacctta tgatgtggtg
      301 gttctaccag gaggtaatct gggcgcacag aatttatctg agtctgctgc tgtgaaggag
      361 atactgaagg agcaggaaaa ccggaagggc ctgatagcca ccatctgtgc aggtcctact
      421 gctctgttgg ctcatgaaat aggttttgga agtaaagtta caacacaccc tcttgctaaa
      481 gacaaaatga tgaatggagg tcattacacc tactctgaga atcgtgtgga aaaagacggc
      541 ctgattctta caagccgggg gcctgggacc agcttcgagt ttgcgcttgc aattgttgaa
      601 gccctgaatg gcaaggaggt ggcggctcaa gtgaaggctc cacttg
//
