LOCUS       sequence-98441-e         690 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Recombinant protein expression of Park7.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 690)
  AUTHORS   Milkovic NM, Catazaro J, Lin J, Halouska S, Kizziah JL, Basiaga S, 
            Cerny RL, Powers R, Wilson MA
  TITLE     Transient sampling of aggregation-prone conformations causes 
            pathogenic instability of a parkinsonian mutant of DJ-1 at 
            physiological temperature.
  JOURNAL   Protein Sci. 2015 Oct;24(10):1671-85. doi: 10.1002/pro.2762. Epub 
            2015 Aug 17.
  PUBMED    26234586
REFERENCE   2  (bases 1 to 690)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/pro.2762"; 
            journalName: "Protein Sci"; date: "2015-10"; volume: "24"; issue: 
            "10"; pages: "1671-85"
COMMENT     Sequence Label: M26V DJ-1 pET15b
FEATURES             Location/Qualifiers
     source          1..690
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             2..24
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             43..60
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             70..87
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 ttttgtttaa ctttaagaag gagatatacc atgggcagca gccatcatca tcatcatcac
       61 agcagcggcc tggtgccgcg cggcagccat atggctttcc aaaagagctc tggtcatcct
      121 ggctaaagga gcagaggaaa tggagacggt catccctgta gatgtcgtga ggcgagctgg
      181 gattaaggtc accgttgcag gcctggctgg aaaagaccca gtacagtgta gccgtgatgt
      241 ggtcatttgt cctgatgcca gccttgaaga tgcaaaaaaa gagggacctt atgatgtggt
      301 ggttctacca ggaggtaatc tgggcgcaca gaatttatct gagtctgctg ctgtgaagga
      361 gatactgaag gagcaggaaa accggaaggg cctgatagcc gccatctgtg caggtcctac
      421 tgctctgttg gctcatgaaa taggttttgg aagtaaagtt acaacacacc ctcttgctaa
      481 agacaaaatg atgaatggag gtcattacac ctactctgag aatcgtgtgg aaaaagacgg
      541 cctgattctt acaagccggg ggcctgggac cagcttcgag tttgcgcttg caattgttga
      601 agccctgaat ggcaaggagg tggcggctca agtgaaggct ccacttgttc ttaaagacta
      661 gctcgaggat ccggctgcta acaaagcccg
//
