LOCUS       sequence-98442-e         689 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Recombinant protein expression of Park7.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 689)
  AUTHORS   Milkovic NM, Catazaro J, Lin J, Halouska S, Kizziah JL, Basiaga S, 
            Cerny RL, Powers R, Wilson MA
  TITLE     Transient sampling of aggregation-prone conformations causes 
            pathogenic instability of a parkinsonian mutant of DJ-1 at 
            physiological temperature.
  JOURNAL   Protein Sci. 2015 Oct;24(10):1671-85. doi: 10.1002/pro.2762. Epub 
            2015 Aug 17.
  PUBMED    26234586
REFERENCE   2  (bases 1 to 689)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/pro.2762"; 
            journalName: "Protein Sci"; date: "2015-10"; volume: "24"; issue: 
            "10"; pages: "1671-85"
COMMENT     Sequence Label: M26V DJ-1 pET15b
FEATURES             Location/Qualifiers
     source          1..689
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(592..609)
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             complement(619..636)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             655..677
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 agcagccgga tcctcgagct agtctttaag aacaagtgga gccttcactt gagccgccac
       61 ctccttgcca ttcagggctt caacaattgc aagcgcaaac tcgaagctgg tcccaggccc
      121 ccggcttgta agaatcaggc cgtctttttc cacacgattc tcagagtagg tgtaatgacc
      181 tccattcatc attttgtctt tagcaagagg gtgtgttgta actttacttc caaaacctat
      241 ttcatgagcc aacagagcag taggacctgc acagatggcg gctatcaggc ccttccggtt
      301 ttcctgctcc ttcagtatct ccttcacagc agcagactca gataaattct gtgcgcccag
      361 attacctcct ggtagaacca ccacatcata aggtccctct ttttttgcat cttcaaggct
      421 ggcatcagga caaatgacca catcacggct acactgtact gggtcttttc cagccaggcc
      481 tgcaacggtg accttaatcc cagctcgcct cacgacatct acagggatga ccgtctccat
      541 ttcctctgct cctttagcca ggatgaccag agctcttttg gaagccatat ggctgccgcg
      601 cggcaccagg ccgctgctgt gatgatgatg atgatggctg ctgcccatgg tatatctcct
      661 tcttaaagtt aaacaaaatt atttctaga
//
