LOCUS       sequence-98443-e        1080 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmids is a modular mini-Tn5 vector that could be used to 
            generate random mutagenesis libraries or to insert heterologous DNA 
            into a target genome .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1080)
  AUTHORS   Martinez-Garcia E, Calles B, Arevalo-Rodriguez M, de Lorenzo V
  TITLE     pBAM1: an all-synthetic genetic tool for analysis and construction 
            of complex bacterial phenotypes.
  JOURNAL   BMC Microbiol. 2011 Feb 22;11:38. doi: 10.1186/1471-2180-11-38.
  PUBMED    21342504
REFERENCE   2  (bases 1 to 1080)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Microbiol."; date: "2011-02-22"; volume: "11:38. doi"; pages: " 
            10.1186/1471-2180-11-38"
COMMENT     Sequence Label: pBAM1
FEATURES             Location/Qualifiers
     source          1..1080
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    85..103
                     /label=Tn5 ME
                     /note="hyperactive mosaic end for Tn5 transposase 
                     recognition (Reznikoff et al., 2004)"
ORIGIN
        1 tgtaagcaga cagttttatt gttcatgatg atatattttt atcttgtgca atgtaacatc
       61 agagattttg agacacaaga cgtcagatgt gtataagaga cagctggttt aaactacaca
      121 agtagcgtcc tgaacggaac ctttcccgtt ttccagaatc tgatgttcca tgtgacctcc
      181 taacatggta acgttcatga ttaccagtgc actgcatcgt gcggcggatt gggcgaaaag
      241 cgtgttttct agtgctgcgc tgggtgatcc gcgtcgtacc gcgcgtctgg tgaatgttgc
      301 ggcgcaactg gccaaatata gcggcaaaag cattaccatt agcagcgaag gcagcaaagc
      361 catgcaggaa ggcgcgtatc gttttattcg taatccgaac gtgagcgcgg aagcgattcg
      421 taaagcgggt gccatgcaga ccgtgaaact ggcccaggaa tttccggaac tgctggcaat
      481 tgaagatacc acctctctga gctatcgtca tcaggtggcg gaagaactgg gcaaactggg
      541 tagcattcag gataaaagcc gtggttggtg ggtgcatagc gtgctgctgc tggaagcgac
      601 cacctttcgt accgtgggcc tgctgcatca agaatggtgg atgcgtccgg atgatccggc
      661 ggatgcggat gaaaaagaaa gcggcaaatg gctggccgct gctgcaactt cgcgtctgag
      721 aatgggcagc atgatgagca acgtgattgc ggtgtgcgat cgtgaagcgg atattcatgc
      781 gtatctgcaa gataaactgg cccataacga acgttttgtg gtgcgtagca aacatccgcg
      841 taaagatgtg gaaagcggcc tgtatctgta tgatcacctg aaaaaccagc cggaactggg
      901 cggctatcag attagcattc cgcagaaagg cgtggtggat aaacgtggca aacgtaaaaa
      961 ccgtccggcg cgtaaagcga gcctgagcct gcgtagcggc cgtattaccc tgaaacaggg
     1021 caacattacc ctgaacgcgg tgctggccga agaaattaat ccgccgaaag gcgaaacccc
//
