LOCUS       sequence-98445-e         616 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Recombinant protein expression of Park7.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 616)
  AUTHORS   Milkovic NM, Catazaro J, Lin J, Halouska S, Kizziah JL, Basiaga S, 
            Cerny RL, Powers R, Wilson MA
  TITLE     Transient sampling of aggregation-prone conformations causes 
            pathogenic instability of a parkinsonian mutant of DJ-1 at 
            physiological temperature.
  JOURNAL   Protein Sci. 2015 Oct;24(10):1671-85. doi: 10.1002/pro.2762. Epub 
            2015 Aug 17.
  PUBMED    26234586
REFERENCE   2  (bases 1 to 616)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/pro.2762"; 
            journalName: "Protein Sci"; date: "2015-10"; volume: "24"; issue: 
            "10"; pages: "1671-85"
COMMENT     Sequence Label: M26I DJ-1 TEV site pET15b
FEATURES             Location/Qualifiers
     source          1..616
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             29..46
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             56..73
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             80..100
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 aagaaggaga tataccatgg gcagcagcca tcatcatcat catcacagca gcggcctggt
       61 gccgcgcggc agccatatgg aaaacctgta ttttcagtct gcttccaaaa gagctctggt
      121 catcctggct aaaggagcag aggaaatgga gacggtcatc cctgtagatg tcatcaggcg
      181 agctgggatt aaggtcaccg ttgcaggcct ggctggaaaa gacccagtac agtgtagccg
      241 tgatgtggtc atttgtcctg atgccagcct tgaagatgca aaaaaagagg gaccttatga
      301 tggggtggtt ctaccaggag gtaatctggg cgcacagaat ttatctgagt ctgctgctgt
      361 gaaggagata ctgaaggagc aggaaaaccg gaagggcctg atagccgcca tctgtgcagg
      421 tcctactgct ctgttggctc atgaaatagg ttttggaagt aaagttacaa cacaccctct
      481 tgctaaagac aaaatgatga atggaggtca ttacacctac tctgagaatc gtgtggaaaa
      541 agacggcctg attcttacaa gccgggggcc tgggaccagc ttcgagtttg cgcttgcaat
      601 tgttgaaagc cctgaa
//
