LOCUS       sequence-98447-e         690 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Recombinant protein expression of Park7.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 690)
  AUTHORS   Milkovic NM, Catazaro J, Lin J, Halouska S, Kizziah JL, Basiaga S, 
            Cerny RL, Powers R, Wilson MA
  TITLE     Transient sampling of aggregation-prone conformations causes 
            pathogenic instability of a parkinsonian mutant of DJ-1 at 
            physiological temperature.
  JOURNAL   Protein Sci. 2015 Oct;24(10):1671-85. doi: 10.1002/pro.2762. Epub 
            2015 Aug 17.
  PUBMED    26234586
REFERENCE   2  (bases 1 to 690)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/pro.2762"; 
            journalName: "Protein Sci"; date: "2015-10"; volume: "24"; issue: 
            "10"; pages: "1671-85"
COMMENT     Sequence Label: M26I DJ-1 TEV site pET15b
FEATURES             Location/Qualifiers
     source          1..690
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(593..613)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             complement(620..637)
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             complement(647..664)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 ctttgttagc agccggatcc tcgagctagt ctttaagaac aagtggagcc ttcacttgag
       61 ccgccacctc cttgccattc agggcttcaa caattgcaag cgcaaactcg aagctggtcc
      121 caggcccccg gcttgtaaga atcaggccgt ctttttccac acgattctca gagtaggtgt
      181 aatgacctcc attcatcatt ttgtctttag caagagggtg tgttgtaact ttacttccaa
      241 aacctatttc atgagccaac agagcagtag gacctgcaca gatggcggct atcaggccct
      301 tccggttttc ctgctccttc agtatctcct tcacagcagc agactcagat aaattctgtg
      361 cgcccagatt acctcctggt agaaccacca catcataagg tccctctttt tttgcatctt
      421 caaggctggc atcaggacaa atgaccacat cacggctaca ctgtactggg tcttttccag
      481 ccaggcctgc aacggtgacc ttaatcccag ctcgcctgat gacatctaca gggatgaccg
      541 tctccatttc ctctgctcct ttagccagga tgaccagagc tcttttggaa gcagactgaa
      601 aatacaggtt ttccatatgg ctgccgcgcg gcaccaggcc gctgctgtga tgatgatgat
      661 gatggctgct gcccatggta tatctccttc
//
