LOCUS       sequence-98458-e         902 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Disintegrator plasmid encoding MET17 promoter driven yellow beetle 
            luciferase with nuclear localization signal (NLS) and ClpXP degron 
            tag (ssrA)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 902)
  AUTHORS   Mazo-Vargas A, Park H, Aydin M, Buchler NE
  TITLE     Measuring fast gene dynamics in single cells with time-lapse 
            luminescence microscopy
  JOURNAL   Mol Biol Cell. 2014 Sep 17. pii: mbc.E14-07-1187.
  PUBMED    25232010
REFERENCE   2  (bases 1 to 902)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Biol 
            Cell. 2014 Sep 17. pii: mbc.E14-07-1187."
COMMENT     Sequence Label: pNB693
FEATURES             Location/Qualifiers
     source          1..902
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             44..64
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(149..167)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
ORIGIN
        1 attctattac ccccatccat actcttaatt aaccatggct ccaccaaaaa aaaaaagaaa
       61 agttgccatg ggtgggggca gcatggaaga cgccaaaaac ataaagaaag gcccggcgcc
      121 attctatcct ctagaggatg gaaccgctgg agagcaactg cataaggcta tgaagagata
      181 cgccctggtt cctggaacaa ttgcttttac agatgcacat atcgaggtga acatcacgta
      241 cgcggaatac ttcgaaatgt ccgttcggtt ggcagaagct atgaaacgat atgggctgaa
      301 tacaaatcac agaatcgtcg tatgcagtga aaactctctt caattcttta tgccggtgtt
      361 gggcgcgtta tttatcggag ttgcagttgc gcccgcgaac gacatttata atgaacgtga
      421 attgctcaac agtatgaaca tttcgcagcc taccgtagtg tttgtttcca aaaaggggtt
      481 gcaaaaaatt ttgaacgtgc aaaaaaaatt accaataatc cagaaaatta ttatcatgga
      541 ttctaaaacg gattaccagg gatttcagtc gatgtatacg ttcgtcacat ctcatctacc
      601 tcccggtttt aatgaatacg attttgtacc agagtccttt gatcgtgaca aaacaattgc
      661 actgataatg aattcctctg gatctactgg gttacctaag ggtgtggccc ttccgcatag
      721 aactgcctgc gtcagattct cgcatgccag agatcctatt tttggcaatc aaatcattcc
      781 ggatactgcg attttaagtg ttgttccatt ccatcacggt tttggaatgt ttactacact
      841 cggatatttg atatgtggat ttcgagtcgt cttaatgtat agatttgaag aagagctgtt
      901 tt
//
