LOCUS       sequence-98466-e        1537 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses a tandem ddFP heterodimer in mammalian cells, plasmid 1 
            for a translocation-based red fluorescent caspase-8 biosensor, used 
            together with plasmid 2 (BNLS)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1537)
  AUTHORS   Ding Y, Li J, Enterina JR, Shen Y, Zhang I, Tewson PH, Mo GC, Zhang 
            J, Quinn AM, Hughes TE, Maysinger D, Alford SC, Zhang Y, Campbell RE
  TITLE     Ratiometric biosensors based on dimerization-dependent fluorescent 
            protein exchange.
  JOURNAL   Nat Methods. 2015 Jan 26. doi: 10.1038/nmeth.3261.
  PUBMED    25622108
REFERENCE   2  (bases 1 to 1537)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2015 Jan 26. doi: 10.1038/nmeth.3261."
COMMENT     Sequence Label: RANLS-IETD-BNES
FEATURES             Location/Qualifiers
     source          1..1537
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             719..739
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             743..763
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             767..787
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 ctcgagaatg gtgagcaaga gcgaggaggt catcaaagag ttcatgcgct tcaaggtgcg
       61 cttggagggc tccatgaacg gccacgagtt cgagatcgag ggcgagggcg agggccgccc
      121 ctacgagggc acccagaccg ccaagctgaa ggtgaccaag ggcggccccc tgcccttcgc
      181 ctgggacatc ctgtcccccc agatcatgta cggctccaag gcgtacgtga agcaccccgc
      241 cgacgtcccc gattacatga agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat
      301 gcacttcgag gacggcggtc tggtgaccgt gactcaggac acctccctgc aggacggcac
      361 gctgatctac aaggtgaaga tgcgcggcac caacttcccc cccgacggcc ccgtaatgca
      421 gaagaagacc ttgggctggg attatgccac cgagcgcctg taccccgaag acggcgtgct
      481 gaagggcgag cttctggggc gcctgaagct gaaggacggc ggcctcaacc tggtggagtt
      541 caagaccatc tacatggcca agaagcccgt gcaactgccc ggctactact tcgtggacac
      601 caagctgggc atcacctccc acaacgagga ctacaccatc gtggaacagt acgagcgctc
      661 cgagggccgc caccacctgg gcatggacga gctgtacaag ggaacggcgg gaagcgatcc
      721 aaaaaagaag agaaaggtag atccaaaaaa gaagagaaag gtagatccaa aaaagaagag
      781 aaaggtaggt accgcctccg gcattgagac ggatggatcc accatcaaag agttcatgcg
      841 cttcaaggtg cgcatggagg gctccatgaa cggccacgag ttcgagatcg agggcgaggg
      901 cgagggccgc ccctacgagg gcacccagac cgccaagctg aaggtgacca agggcggccc
      961 cctgcccttc gcctgggaca tcctgtcccc ccagttcatg tacggctccg aggcgtacgt
     1021 gaggcacccc gccgacatcc ccgattacaa gaagctgccc ttccccgagg gcttcaagtg
     1081 ggagcgcgtg atgaacttcg aagacggcgg tctggtgacc gttacccagg actcctccct
     1141 gcaggacggc acgctgatct gcaaggtgaa gatgcgcggc accaacttcc cccccgacgg
     1201 ccccgtaatg cagaagaaga ccatgggctg ggaggcctcc accgagatgc tgtaccccga
     1261 agacggcgtg ctgaagggcc atagctatca ggccctgaag ctgaaggacg gcggccacta
     1321 cctggtggag ttcgagacca tctacatggc caagaagccc gtgcaactgc ccggcgatta
     1381 ctgtgtggac accaagctgg acatcacctc ccacaacgag gactacacca tcgtggaaca
     1441 gtacgggcgc tccgagggcc gccaccgtct gggcatggac gagcggtaca agttagcctt
     1501 gaaattagca ggtcttgata tcgggagcta aaagctt
//
