LOCUS       sequence-98468-e        1993 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  For imaging of ERK kinase activity using a single polypeptide RA-WW 
            domain-ERK substrate-B protein and free GA-NES..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1993)
  AUTHORS   Ding Y, Li J, Enterina JR, Shen Y, Zhang I, Tewson PH, Mo GC, Zhang 
            J, Quinn AM, Hughes TE, Maysinger D, Alford SC, Zhang Y, Campbell RE
  TITLE     Ratiometric biosensors based on dimerization-dependent fluorescent 
            protein exchange.
  JOURNAL   Nat Methods. 2015 Jan 26. doi: 10.1038/nmeth.3261.
  PUBMED    25622108
REFERENCE   2  (bases 1 to 1993)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2015 Jan 26. doi: 10.1038/nmeth.3261."
COMMENT     Sequence Label: Ratiometric FPX sensor for ERK kinase activity
FEATURES             Location/Qualifiers
     source          1..1993
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             872..1219
                     /codon_start=1
                     /product="long flexible linker consisting of SAGG repeats 
                     (Komatsu et al., 2011)"
                     /label=EV linker
                     /translation="SAGGSAGGSAGGSAGGSAGGSGSAGGSAGGSTSAGGSAGGSAGGS
                     AGGSAGGSGSAGGSAGGSTSAGGSAGGSAGGSAGGSAGGSGSAGGSAGGSTSAGGSAGG
                     SAGGSAGGSAGG"
ORIGIN
        1 ggatcccatg gtgagcaaga gcgaggaggt catcaaagag ttcatgcgct tcaaggtgcg
       61 cttggagggc tccatgaacg gccacgagtt cgagatcgag ggcgagggcg agggccgccc
      121 ctacgagggc acccagaccg ccaagctgaa ggtgaccaag ggcggccccc tgcccttcgc
      181 ctgggacatc ctgtcccccc agatcatgta cggctccaag gcgtacgtga agcaccccgc
      241 cgacgtcccc gattacatga agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat
      301 gcacttcgag gacggcggtc tggtgaccgt gactcaggac acctccctgc aggacggcac
      361 gctgatctac aaggtgaaga tgcgcggcac caacttcccc cccgacggcc ccgtaatgca
      421 gaagaagacc ttgggctggg attatgccac cgagcgcctg taccccgaag acggcgtgct
      481 gaagggcgag cttctggggc gcctgaagct gaaggacggc ggcctcaacc tggtggagtt
      541 caagaccatc tacatggcca agaagcccgt gcaactgccc ggctactact tcgtggacac
      601 caagctgggc atcacctccc acaacgagga ctacaccatc gtggaacagt acgagcgctc
      661 cgagggccgc caccacctgg ggcatggcac cggcagcaga tctatggcgg acgaggagaa
      721 gctgccgccc ggctgggaga agcgcatgag ccgcagctca ggccgagtgt actacttcaa
      781 ccacatcact aacgccagcc agtgggagcg gcccagcggc aacagcagca gtggtggcaa
      841 aaacgggcag ggggagcctg ccaggggtac cagtgctggt ggtagtgctg gtggtagtgc
      901 tggtggtagt gctggtggta gtgctggtgg ttccggcagt gctggtggta gtgctggtgg
      961 tagtaccagt gctggtggta gtgctggtgg tagtgctggt ggtagtgctg gtggtagtgc
     1021 tggtggttcc ggcagtgctg gtggtagtgc tggtggtagt accagtgctg gtggtagtgc
     1081 tggtggtagt gctggtggta gtgctggtgg tagtgctggt ggttccggca gtgctggtgg
     1141 tagtgctggt ggtagtacca gtgctggtgg tagtgctggt ggtagtgctg gtggtagtgc
     1201 tggtggtagt gctggtggtt ccggaccaga tgtccctaga actccagtgg ataaagcaaa
     1261 gctgtcattc caatttccgg gtaccgcctc ctccgaggac aacaacatgg ccaccatcaa
     1321 agagttcatg cgcttcaagg tgcgcatgga gggctccatg aacggccacg agttcgagat
     1381 cgagggcgag ggcgagggcc gcccctacga gggcacccag accgccaagc tgaaggtgac
     1441 caagggcggc cccctgccct tcgcctggga catcctgtcc ccccagttca tgtacggctc
     1501 cgaggcgtac gtgaggcacc ccgccgacat ccccgattac aagaagctgc ccttccccga
     1561 gggcttcaag tgggagcgcg tgatgaactt cgaagacggc ggtctggtga ccgttaccca
     1621 ggactcctcc ctgcaggacg gcacgctgat ctgcaaggtg aagatgcgcg gcaccaactt
     1681 cccccccgac ggccccgtaa tgcagaagaa gaccatgggc tgggaggcct ccaccgagat
     1741 gctgtacccc gaagacggcg tgctgaaggg ccatagctat caggccctga agctgaagga
     1801 cggcggccac tacctggtgg agttcgagac catctacatg gccaagaagc ccgtgcaact
     1861 gcccggcgat tactgtgtgg acaccaagct ggacatcacc tcccacaacg aggactacac
     1921 catcgtggaa cagtacgggc gctccgaggg ccgccaccgt ctgggcatgg acgagcggta
     1981 caagtaagaa ttc
//
