LOCUS       sequence-98473-e         827 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 827)
  TITLE     Johnson Lab Plasmids
REFERENCE   2  (bases 1 to 827)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pcDNA3-IGFBP5-V5
FEATURES             Location/Qualifiers
     source          1..827
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     37..56
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        37..55
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      85..94
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 actagagaac ccactgcctt actggcttat cgaaattaat acgactcact atagggagac
       61 ccaagctggc tagttaagct tgccgccacc atggtgttgc tcaccgcggt cctcctgctg
      121 ctggccgcct atgcggggcc ggcccagagc ctgggctcct tcgtgcactg cgagccctgc
      181 gacgagaaag ccctctccat gtgccccccc agccccctgg gctgcgagct ggtcaaggag
      241 ccgggctgcg gctgctgcat gacctgcgcc ctggccgagg ggcagtcgtg cggcgtctac
      301 accgagcgct gcgcccaggg gctgcgctgc ctcccccggc aggacgagga gaagccgctg
      361 cacgccctgc tgcacggccg cggggtttgc ctcaacgaaa agagctaccg cgagcaagtc
      421 aagatcgaga gagactcccg tgagcacgag gagcccacca cctctgagat ggccgaggag
      481 acctactccc ccaagatctt ccggcccaaa cacacccgca tctccgagct gaaggctgaa
      541 gcagtgaaga aggaccgcag aaagaagctg acccagtcca agtttgtcgg gggagccgag
      601 aacactgccc acccccggat catctctgca cctgagatga gacaggagtc tgagcagggc
      661 ccctgccgca gacacatgga ggcttccctg caggagctca aagccagccc acgcatggtg
      721 ccccgtgctg tgtacctgcc caattgtgac cgcaaaggat tctacaagag aaagcagtgc
      781 aaaccttccc gtggccgcaa gcgtggcatc tgctggtgcg tggacaa
//
