LOCUS       sequence-98479-e         640 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Recombinant protein expression of DJ-1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 640)
  AUTHORS   Madzelan P, Labunska T, Wilson MA
  TITLE     Influence of peptide dipoles and hydrogen bonds on reactive cysteine
            pKa values in fission yeast DJ-1.
  JOURNAL   FEBS J. 2012 Nov;279(22):4111-20. doi: 10.1111/febs.12004. Epub 2012
            Oct 11.
  PUBMED    22971103
REFERENCE   2  (bases 1 to 640)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1111/febs.12004"; journalName: "FEBS J"; date: "2012-11"; 
            volume: "279"; issue: "22"; pages: "4111-20"
COMMENT     Sequence Label: SpDJ-1 pET15b
FEATURES             Location/Qualifiers
     source          1..640
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             10..32
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             78..95
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 tctgaatatt ttgtttaact ttaagaagga gatataccat gggcagcagc catcatattc
       61 atcatcacag cagcggcctg gtgccgcgcg gcagccatat ggttaaagtg tgcctgtttg
      121 ttgcagatgg caccgatgaa attgaattta gcgcaccgtg gggtattttt aaacgtgccg
      181 aaattccgat tgatagcgtg tatgtgggcg aaaataaaga tcgtctggtg aaaatgagcc
      241 gtgatgttga aatgtatgcc aatcgcagct ataaagaaat tccgagcgca gatgattttg
      301 ccaaacagta tgatattgcc attattccgg gtggtggtct gggtgcaaaa accctgagca
      361 ccaccccgtt tgttcagcag gttgtgaaag aattttataa aaaaccgaat aaatggattg
      421 gtatgatttg tgcaggcacc ctgaccgcaa aaaccagcgg tctgccgaat aaacaaatta
      481 ccggtcatcc gagcgttcgt ggtcagctgg aagaaggtgg ctataaatat ctggatcagc
      541 cggttgttct ggaagaaaat ctgattacca gccagggtcc gggtacagca atgctgtttg
      601 gtctgaaatg ctggaacagg tggcaagcta cgataaatat
//
