LOCUS       sequence-98484-e         668 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Recombinant protein expression of YajL.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 668)
  AUTHORS   Wilson MA, Ringe D, Petsko GA
  TITLE     The atomic resolution crystal structure of the YajL (ThiJ) protein 
            from Escherichia coli: a close prokaryotic homologue of the 
            Parkinsonism-associated protein DJ-1.
  JOURNAL   J Mol Biol. 2005 Oct 28;353(3):678-91. Epub 2005 Sep 2.
  PUBMED    16181642
REFERENCE   2  (bases 1 to 668)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Mol 
            Biol."; date: "2005-10-28"; volume: "353(3)"; pages: "678-91. Epub 
            2005 Sep 2"
COMMENT     Sequence Label: EcYajL pET15b
FEATURES             Location/Qualifiers
     source          1..668
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(617..634)
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             complement(644..661)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 gttagcagcc ggtatcctcg agctactcgt aataattata aatccctgcc gccatcacca
       61 gttgtgatgc cacttcatgg gctttttcac gcccaaccaa caggtcgata attttcagac
      121 caaagtcgat agctgtaccc ggcccctggc tggtcagcaa ttttacccgt gcatcccaga
      181 cgacgcgctt gtccagccat tgttcggcgg gaattttgtc tttcagcgtc gggaagccgg
      241 tcatattacc aatcgggaag atatcgtgcg gcaccagcac ggtggctggc gcggcgcaaa
      301 tagccgcgac gatacgcccg gaacggtgga actgtttaac ggtttcaacc agcagagtgc
      361 tatcgcgaaa acactccgcg cctttaatgc caccaggcag cacgatcacg tcatattcgc
      421 catcagccac ttcgaccagc ggcgcatccg ccagcagctt cacgccgcgc gagcaggtaa
      481 tcgccaggtt accatcgctg gcgacgctgg cagtggtgac tttgataccg ccgcgaacca
      541 gcagatcgat agtggtgacg gcttcagtct cttcactacc aggggcgagg caaaccagtg
      601 ccgatgcgct catatggctg ccgcgcggca ccaggccact gctgtgatga tgatgatgat
      661 ggctgctg
//
